培养条件对康宁木霉产酶的影响

通过对康宁木霉纤维素酶（C1酶和Cx酶）、酸性蛋白酶活力的比较,筛选出了康宁木霉产酶的最适培养条件.康宁木霉在m（麸皮）∶m（秸秆）=2∶8,氮源为w=1%的NH4NO3,含水量为65%,起始pH值为6.5的条件下培养72h,C1酶活力最高,为191.3U/g;在m（麸皮）∶m（秸秆）=6∶4,氮源为w=1%的（NH4）2SO4,含水量为65%,起始pH值为7.5的条件下培养48h,Cx酶活力最高,为2006.5U/g;在m（麸皮）∶m（秸秆）=8∶2,氮源为w=1%的（NH4）2CO3,含水量为65%,起始pH值为4.5的条件下培养96h,酸性蛋白酶活力最高,为1859.6U/g.     

锇聚合物修饰丝网印刷电极的辣根过氧化物酶传感器

酶生物传感器检测环境污染应用前景良好。该文采用戊二醛-牛血清白蛋白交联法将锇-聚乙烯基吡啶(Os(bpy)2(PVP)10Cl2,Os-PVP)与辣根过氧化物酶(HRP)依次固定在丝网印刷电极上,制备电流型过氧化氢生物传感器。通过循环伏安法对修饰电极的氧化还原性质进行研究,并采用计时-电流法对传感器的固定化和工作参数进行了研究,以便提高信号响应的灵敏度和稳定性。在最适合条件下,底物在修饰电极表面进行催化反应的表观Michaelis-Menten常数kampp为1.79mmol/L,并确定以HRP酶为标记物的免疫传感器所采用的底物浓度宜大于20倍的kmapp。     

芹菜根对模拟复合污染湿地中芘的降解作用

以吴淞口复合污染湿地土壤为研究背景, 在含有芘、柴油、Pb 的模拟复合污染湿地土壤环境中, 选取对芘具有高效降解作用的芹菜根, 研究芹菜根对复合污染湿地土壤进行生态修复的作用效果及特征. 检测分析了单一与复合污染土壤中多酚氧化酶(polyphenol oxidase, PPO)、过氧化氢酶(catalase, CAT)、脲酶(urease, URE)及不同实验组中芘的降解效果随时间的变化规律. 研究表明, 在芘、Pb、柴油复合污染胁迫下, 芹菜根可以通过根系分泌酶等一些化学物质或者通过刺激其根际微生物细胞分泌各种酶类物质, 来提高其根际微生物的活性, 促进芹菜根与其根际微生物的协同作用, 增强对复合污染土壤中芘的降解效果.     

肥胖以及有氧耐力运动对大鼠血清抗氧化酶和一氧化氮的影响

研究了肥胖以及耐力性运动对大鼠血清抗氧化酶和一氧化氮水平的影响,结果表明,高脂饮食组实验大鼠血清中SOD和CAT浓度显著性下降,MDA明显增加,NO活性下降;高脂饮食结合有氧耐力运动组大鼠血清中SOD和CAT活性以及MDA明显上升,血清中NO水平恢复明显.肥胖大鼠体重增长被明显抑制.结合生化试验结果,确定高脂饮食诱发肥胖后可造成实验动物血清中抗氧化酶活性下降,自由基生成增加,血管舒张功能下降,系统的有氧耐力运动可以有效的逆转高脂饮食造成的自由基增加,NO恢复至正常水平.     

采用蛋白酶组合漂白技术对羊毛增白的探讨

根据厂家对羊毛织物白度要求，需要开发一种快速，安全，价廉的羊毛漂白生产技术以满足于生产研究，研究了蛋白关正常氧漂工艺中对织物白度的促进作用及采取正确处理方法是获得良好的必要条件。     

Knitting and Finishing of Ramie Knitted Fabrics Blended with Fine Polyester

Ramie fibre is harsh and rigid. Ramie yarns have a hairy feel and reduced cohesion. A considerable amount of research has been done to ease the undesirable properties of ramie products through modifying conditions of knitting and finishing according to the special properties of the ramie yarn. From the comparison results, burning the hairiness is shown as one of the effective methods to bnprove the properties of the yarn. A typical finishing condition with enzyme treatment is also recommended.     

Sugar-mimicking glycosidase inhibitors: bioactivity and application

A large number of compounds mimicking the structures of monosaccharides or oligosaccharides have been discovered from natural sources. Such sugar mimics inhibit carbohydrate-degrading enzymes because of a structural resemblance to the sugar moiety of the natural substrate. Carbohydrate-degrading enzymes are involved in a wide range of important biological processes, such as intestinal digestion, posttranslational processing of the sugar chain of glycoproteins, their quality control mechanisms, lysosomal catabolism of glycoconjugates, and some viral infections. It has now been realized that inhibitors of the enzymes have enormous therapeutic potential in diabetes and lysosomal storage disorders. In this review, the general bioactivity, current applications, and the prospects for new therapeutic applications are described. Received 27 August 2008; received after revision 08 November 2008; accepted 03 December 2008     

碱性蛋白酶催化的Henry反应

Henry反应是一类重要的碳碳键形成反应，广泛用于各类天然产物和医药中间体合成中．使用碱性蛋白酶催化芳香醛和硝基甲烷之间的Henry反应，在室温的条件下，得到对应的产物，产率高达88％．     

Systematics and evolution of holarctic Pierinae (Lepidoptera). An enzyme electrophoretic approach

Summary Enzyme electrophoretic data show a remarkably high degree of genetic similarity within the European group ofnapi s.l. whereas genetic differences exist at several loci between the European and the North American taxa ofnapi s.l. It is concluded that the European taxa did not differentiate to the species level and form a phylogenetically young group. The North American taxa included in this study are specifically distinct from Europeannapi and separated much earlier. Within these North American taxamarginalis, oleracea andvirginiensis did undergo speciation. The data show a splitting of the genusPieris into three species groups, each genetically differentiated to the same level. The splitting ofPieris into two genera, as suggested by earlier investigators, is not supported here.