Comparative proteomic analysis of the response in resistant and susceptible maize inbred lines to infection by Curvularia lunata

Proteins differentially expressed from maize leaves in response to the infection by Curvularia lunata strain CX-3 were identified through a high-resolution two-dimensional gel electrophoresis (2-DE) method. Two inbred lines, 78599-1 and E28, were used, respectively, as resistant and susceptible lines to CX-3 infection. Proteins were extracted from the fourth leaves of six- or seven-leaf stage plants sampled at 24, 36, 48, 60, and 72 h after inoculation with CX-3. Twenty-seven differentially expressed protein spots resolved on the 2-DE gels were identified by MALDI-TOF MS/MS. The results showed that these proteins are associated with photosynthesis, respiration,oxidative and drought stress tolerance as well as signal transduction in maize. Among stress-related proteins, the 22 kDa drought-inducible protein, putative glutathione peroxidase (GPX), and translation initiation factor (eIF-5A) were up-regulated in the resistant inbred line and were implicated in host defense response to C. lunata infection. It suggests that drought-inducible and oxidation stress-related proteins might directly contribute to maize resistance to C. lunata.     

东亚飞蝗不同龄期解毒酶和靶标酶活性研究

对东亚飞蝗(Locusta migratoria manilensis(Meyen))各龄期酯酶、乙酰胆碱酯酶、谷胱甘肤 S-转移酶活性进行了比较研究,同时对酯酶进行了非变性聚丙烯酰胺凝胶检测.酯酶活性测定结果表明,不同龄期酯酶的比活力不同,五龄若虫最高,一龄若虫最低.酯酶非变性聚丙烯酰胺凝胶电泳结果发现,各龄期酯酶同工酶酶谱主带比较一致,在谱带上存在有细微的差异;乙酰胆碱酯酶的比活力二龄若虫最高,四龄若虫最低;一龄若虫谷胱甘肽S-转移酶的比活力最高,三龄若虫最低.据此推测,东亚飞蝗不同龄期解毒酶和靶标酶的活性差异可能是由于各龄期生长发育代谢机能的不同所致.     

腹腔注射板蓝根多糖对中华鳖肝脏抗氧化酶活性和丙二醛含量的影响

研究了腹腔注射板蓝根多糖对中华鳖肝脏抗氧化性的影响。实验共分4组：每天注射1.5?mg/只组、3?mg/只组、6?mg/只组和注射生理盐水对照组,连续注射3?d。结果表明：3个板蓝根多糖处理组均能提高SOD、CAT、 GSH PX的活性,降低MDA的含量,但存在着差异。与对照组相比,3个处理组均能显著提高CAT的活性,而且随注射剂量的增多,活性增强。3?mg/只、6?mg/只剂量组能显著增加GSH PX活性,显著降低MDA含量,但3?mg/只?剂量组影响最大。而1.5、3?mg/只剂量组对SOD的影响不显著,6?mg/只剂量组影响最大而且差异极显著。提示板蓝根多糖能增强中华鳖肝脏的抗氧化性。     

肝癌细胞对三氧化二砷诱导凋亡的敏感性与细胞内谷胱甘肽含量的关系

目的:比较肝癌BEL-7402和SMMC-7721细胞对三氧化二砷(As2O3)诱导凋亡的敏感性差异,探讨细胞内谷胱甘肽(GSH)含量与其作用的关系。方法:不同浓度As2O3作用BEL-7402和SMMC-7721细胞24~96 h,流式细胞仪检测细胞DNA含量,计算细胞凋亡百分率;GSH检测试剂盒检测细胞内GSH含量。结果:0.25μmol/L As2O3作用24 h,有效地诱导BEL-7402细胞凋亡;对SMMC-7721细胞来说,需要用1.0μmol/L As2O3作用24 h才能达到相同的抑制效果。1.0μmol/LAs2O3作用72 h时,BEL-7402细胞的凋亡率为(43.8±0.2)%,SMMC-7721细胞的凋亡率为(12.8±0.2)%,检测BEL-7402细胞内GSH为(18.7±1.4)nmol/mg;SMMC-7721细胞内GSH为(50.8±5.2)nmol/mg,两者比较均有显著差异。结论:肝癌BEL-7402和SMMC-7721细胞对As2O3诱导细胞凋亡的敏感性不同,可能与细胞内GSH含量有关。     

GST酶的提取纯化及特性分析

通过硫酸铵分级盐析、Sephadex G-25 脱盐、A-25柱层析、丙酮沉淀、Sephadex G-75及Sephadex G-200等一系列分离纯化手段,从小麦幼穗抽提液中分离得到了电泳纯的谷胱甘肽转移酶.结果表明,1-氯-2,4-二硝基苯(CDNB)浓度变化的体系中,该酶表观Km=10.42 nmol/L;谷胱甘肽(GSH)浓度变化的体系中,表观Km=299 μmol/L.     

流动注射电致化学发光测定谷胱甘肽

发现氧化型谷胱甘肽能增强钌联吡啶的电致化学发光, 而还原型谷胱甘肽抑制钌联吡啶的电致化学发光． 根据增强或抑制程度的大小, 可定量测定谷胱甘肽．     

谷胱甘肽发酵生产及其在动物饲料工业中的应用

谷胱甘肽是由谷氨酸、半胱氨酸和甘氨酸缩合而成的活性三肽。用改造的酵母菌发酵制备是工业化生产谷胱甘肽的较好方法。在动物保健领域,谷胱甘肽作为一种高效且安全的新型饲料添加剂,具有增强免疫、节约饲料、促进生长、改善肉类品质的功能。且其广泛使用能显著减少动物养殖过程中抗生素的用量,保障动物食品安全。     

《JOURNAL OF WUHAN UNIVERSITY （Natural Science Edition） 》Vol.49 No.6，2003 Abstract

Polyaniline (PAn) film was synthesized onto electrode surface modified with a self-assembled monolayer (SAM) of Glutathione (GSH), while copper was deposited in defects of PAn film simultaneously.The resulted PAn films were characterized by means of cyclic voltammetry and electrochemical quartz crystal microbalance (EQCM). The results show that there could be the copper ions‘ route in PAn films, which make easier adulteration of copper ion into the PAn film phase. It was found that the redox peaks of PAn were relevant to the doping of anions and to the adulteration of copper ion.     

微量元素硒与人体健康关系研究的新进展

本文就近二十年来，对硒与人体健康关系的研究进展作一综述。内容包括硒与肿瘤、糖尿病、高血压、男性不育症、克山病等疾病的关系以及作用机理十二个方面的研究，共引用文献58篇。     

亚硒酸钠催化谷胱甘肽、过氧化氢产生超氧阴离子的研究

本文对亚硒酸钠、还原性谷胱甘肽、过氧化氢(或自然溶解氧)进行了研究.采用邻苯三酚自氧化法和电子自旋共振(ESR)技术等均证实了在此反应体系中通过亚硒酸钠的催化作用产生了超氧阴离子(O_2~-)等活性氧自由基.