Screening and optimization of EPA-producing antarctic psychrophilic bacterium Shewanella sp. NJ136

Two hundred strains of bacteria from Antarctic sea ice were collected and screened for their ability of producing eieosapentaenoic acid （EPA, 20： 5ω3） by means of Gas Chromatography/Mass Spetmmetry （GC/MS）. Eight strains of bacteria containing EPA were investigated, among which the outstanding one was recorded as NJ136. This bacterium was identified as Shewanella by the biochemical characteristics and 16S rRNA sequence. Response surface methodology （RSM） was applied to optimize the medium ingredients. A 24 full factorial central composite design （FFCCD） was employed to determine the naximum EPA production at optimum levels of pH, NaC1, glucose and yeast extract. The predicted optimal combination of media constituents for maximum 14.02 mg/g （ about 1.7-fold increase） EPA production were determined as 30.15%e （m/v） NaC1, 9.98 g/L glucose, 4.42 g/L yeast extract and pH 6.08. The actual experimental results were in agreement with the prediction.     

聚乙烯醇-海藻酸钙作为德氏乳酸杆菌包埋剂的研究

以德氏乳酸杆菌为考察对象,应用响应面分析法(RSM)对聚乙烯醇海藻酸钙凝胶配比进行优化研究.结果表明,制备PVA海藻酸钙复合载体的最佳条件是PVA、海藻酸钠和氯化钙的质量分数分别为6.64%、1.29%和1.97%,固化交联时间为6.48h,预测乳酸发酵72h的产量为112.79g/L.经过120d,40批次的发酵验证,结果与预测值相符,且乳酸发酵72h产量比游离细胞发酵平均提高了56%.     

基于响应面方法的乘员坐姿优化

 随着人机工程学的发展以及消费者对商品的舒适性要求的提高,汽车人机工程学越来越被重视.为了在设计最初阶段确定舒适的乘员乘坐姿态,首先利用RAMSIS软件建立人体模型,根据最初的设计参数模拟乘员乘坐姿态,分析当前姿态下的舒适性;然后利用全因子试验设计方法设计2因素6水平试验36次,根据试验数据,运用响应面方法(RSM)拟合出回归模型;最后将回归模型进行显著性分析,应用回归模型得到优化变量及优化目标的最优值.研究结果表明,优化后不舒适度减少13.3%,舒适性明显提高.     

板坯连铸电磁制动下结晶器内金属流场的数学物理模拟

采用雷诺时均湍流模型并结合水银物理模拟实验, 研究不同磁场配置下板坯结晶器内的金属流动规律, 并以此分析不同磁场配置对工艺过程的影响. 结果表明: 雷诺应力模型(Reynolds stress model, RSM)能够较准确地反映电磁制动(electromagnetic braking, EMBr)下金属流场的变化, 该变化引起水口射流及其对结晶器窄边冲刷强度的改变; 在全幅二段电磁制动下, 上部磁场对液面水平速度和湍动性的抑制作用明显, 但从化渣和降低冲击深度的工艺要求来看, 上部磁场强度不宜过大.     

液-液旋流分离管湍流流场的数值模拟

采用雷诺应力模型(RSM)对一种旋流管内部的单相流动进行了湍流数值模拟。通过数值计算得到了旋流管内部流动的速度矢量分布情况,计算结果与经典文献资料吻合,证明了模型的正确,为进一步数值模拟打下了基础。     

响应面方法在可靠性灵敏度计算中的应用

简要叙述了应用响应面方法获取极限状态函数(该极限状态函数具有二次多项式响应面函数的特点)的过程.提出了利用这种极限状态函数进行可靠性灵敏度分析的方法,举例证明该方法可用于极限状态函数未知的情况,并推导了相关计算公式.用响应面方法获取的极限状态函数天然地包括一、二次项和交叉项的信息,不仅使可靠性灵敏度的计算简单易行,而且使计算精度大大提高.最后以热疲劳裂纹为例,计算了其不发生失稳扩展的可靠度及各个随机变量的可靠性灵敏度.     

紫苏籽抗氧化肽的制备工艺研究

采用碱提酸沉法从紫苏籽粕中提取蛋白质,进行蛋白酶筛选,发现紫苏籽蛋白经碱性蛋白酶酶解后有最好的抗氧化活性和水解度(DH).在单因素试验的基础上,采取响应面分析方法,并建立数学模型,确定最佳酶解工艺.试验结果表明:各因素影响紫苏籽粗蛋白酶解液DPPH自由基清除率的主次顺序是:加酶量酶解时间pH值,最佳条件是:加酶量3 000 U·g~(-1),酶解时间5.00 h,pH值9.80,温度50℃,此时DPPH自由基清除率为73.64%,说明紫苏籽蛋白质酶解多肽拥有很高的抗氧化活性.     

Effects of Influencing Factors on Fenton Oxidation of Antibiotic Pharmaceutical Wastewater by a Statistical Design Approach

A series of batch-scale experiments were completed to investigate the effects of operational parameters on chemical oxygen demand (COD) removal by Fenton reagent for antibiotic pharmaceutical wastewater (APW). The significance of five operational factors including the mass ratio of H2O2/COD (g/g), the mole ratio of H2O2/Fe2+ (mol/mol), initial pH, oxidation temperature T, and reaction time t were evaluated statistically by Box-Behnken design (BBD). It was found that the five parameters were all significant to the COD removal efficiency by t-test, as well as the interactions between mass ratio/reaction time and oxidation temperature/reaction. The optimal COD removal efficiency (89.50%) was achieved when the mass ratio of H2O2/COD and the mole ratio of H2O2/Fe2+ were 3.00 and 5.00 respectively, with pH value of 3.68 at 298K for 72min reaction. A quadratic regression model with 0.9907 regression coefficient (R2) was developed which had good agreement to the experimental data.     

响应面优化多刺绿绒蒿总生物碱提取工艺

利用响应面法优化多刺绿绒蒿中总生物碱的提取工艺。在单因素试验的基础上,选择盐酸质量分数、液料比、提取温度为自变量,以总生物碱吸光度为响应值,进行Box-Behnken中心组合实验设计,采用响应面法(RSM)分析这些因素对总生物碱提取的影响。响应面优化设计得出的最佳工艺条件为盐酸质量分数3.7%,液料比38∶1,提取温度56℃,总生物碱提取率达1.18%。