银鲫人工杂交试验中雄核发育现象的RAPD实验证据

以随机扩增多态性ＤＮＡ（ＲＡＰＤ）技术为手段，对生野鲫，雄性异育银鲫及其杂交ＦＩ代（选择性状接近父本的５％Ｆ１代），父本回交Ｆ１你进行了遗传多态分析，从１５０个随机序列引物中筛选得到９种引物，并用它们可得到３６个用作遗传标记的ＲＡＰＤ多态性条带，其中的２２条父本显性条带均遗传子代，而１４条母本性条带则均未被遗传子代，对两亲本和子代中的ＲＡＰＤ标记条带的分布情况及相似系数分析，以及聚类分析结果均支持     

Toward interoperable bioscience data

To make full use of research data, the bioscience community needs to adopt technologies and reward mechanisms that support interoperability and promote the growth of an open 'data commoning' culture. Here we describe the prerequisites for data commoning and present an established and growing ecosystem of solutions using the shared 'Investigation-Study-Assay' framework to support that vision.     

工艺路线可变车间作业调度的杂合遗传算法

针对工艺路线可变的车间作业调度，提出一种将遗传算法与启发式规则、模拟退火法等搜索方法结合在一起的杂合遗传算法。通过对某双极型集成电路封装企业的车间作业调度仿真，结果表明它是有效可行的。     

再制造系统能力约束下拆卸批量计划优化方法

针对再制造系统中能力约束下的拆卸批量计划问题,应用两阶段启发式遗传算法进行了优化求解.首先对再制造产品结构进行了描述,建立了再制造系统中能力约束下的拆卸批量计划优化模型;其次在不考虑能力约束情况下应用遗传算法求解出初始的拆卸批量计划,其中,染色体编码采用拆卸决策变量来表示,同时对适应度函数进行了线性变换,设计了具有自适应的交叉概率和变异概率;然后应用转移算法对初始得到的批量计划进行了修正,使其符合拆卸能力的约束.大量随机算例的仿真实验说明所提出的算法不论在寻找最优解方面还是在求解速度和稳定性方面,都要大大优于精确算法,能够较好地解决实际生产中面临的拆卸批量计划问题.     

基于准时交货的Job Shop多机调度

提出了一个基于准时交货的Ｊｏｂ－Ｓｈｏｐ多机调度模型，应用模拟退火方法并结合启发式算法，给出求解该模型的一种新方法，通过仿真算例说明了方法的有效性。     

Phenylalanine biosynthesis in Brevibacterium lactofermentum using Escherichia coli genes pheA, aroG and tyrB

Genetic engineering technology to increase the production of L-phenylalanine was used in the study.Three genes encoding the key enzymes involved in the biosynthesis of L-phenylalanine were utilized, in which the gene aroG encodes 3-deoxy-D-arabino-heptulosonate-7-phosphate synthetase (DS); the gene pheA encodes bifunctional enzyme of chorisate mutase (CM) and prephenate dehydratase (PD); and the gene tyrb encodes aminotransferase (AT).The three genes were amplified by polymerase chain reaction (PCR) from the genome of the E. coli mutant strains resistant to fluro-DL-phenylalanine and inserted into the cloning vectors. Then, they were expressed in E. coli and Brevibacterium lactofermentum in a tandem arrangement. The expressed enzymes had high activities in the host cells.