| 银鲫人工杂交试验中雄核发育现象的RAPD实验证据 |
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| 引用本文： | 王俐,吴衡.银鲫人工杂交试验中雄核发育现象的RAPD实验证据[J].复旦学报(自然科学版),1997,36(5):565-570. |
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| 作者姓名： | 王俐 吴衡 |
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| 作者单位： | 复旦大学生物化学系!上海，200433，复旦大学，复旦大学，上海水产研究所，复旦大学 |
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| 基金项目： | 上海青年科技启明星计划资助 |
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| 摘 要： | 以随机扩增多态性ＤＮＡ（ＲＡＰＤ）技术为手段，对生野鲫，雄性异育银鲫及其杂交ＦＩ代（选择性状接近父本的５％Ｆ１代），父本回交Ｆ１你进行了遗传多态分析，从１５０个随机序列引物中筛选得到９种引物，并用它们可得到３６个用作遗传标记的ＲＡＰＤ多态性条带，其中的２２条父本显性条带均遗传子代，而１４条母本性条带则均未被遗传子代，对两亲本和子代中的ＲＡＰＤ标记条带的分布情况及相似系数分析，以及聚类分析结果均支持
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| 关 键 词： | 杂交试验 雄核发育 银卿 RAPD多态条带 育种 |
Involvement of androgenesis in reproduction of crucian carp,Carassiun auratus gibelio,as evidenced by RAPD |
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| Wang Li,Wu Heng,Lu Zhijing,Yu Haoxang,Huang Weida.Involvement of androgenesis in reproduction of crucian carp,Carassiun auratus gibelio,as evidenced by RAPD[J].Journal of Fudan University(Natural Science),1997,36(5):565-570. |
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| Authors: | Wang Li Wu Heng Lu Zhijing Yu Haoxang Huang Weida |
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| Abstract: | The Crucian carp, Carassius uuratus gibelio,is known for its gynogenesis. However, there are morphological evidences that androgenesis might also be involved in its reproductive pathway. In this study, random amplified polymorphic DNA (RAPD) technique was used to analyze the genetic deversity of L?arassius auratus auratus, Carassius auratus gibelio,their F1 progeny and paternal back cross. Nine out of 150 random primers were selected and 36 polymophic DNA bands obtained. Among these 22 bands were paternally dominant and the other 14 bands were maternally dominant. The RAPD profiles and the similarity index estimation results support the hypothesis that both gynogenesis and androgenesis might be involved in the reproduction of the crucian carp Carassius auratus gibelio. |
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| Keywords: | gynogenesis androgenesis allogynogenetic crucian carp RAPD |
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