排序方式: 共有94条查询结果,搜索用时 46 毫秒
21.
从猪蛔虫提取总RNA,反转录成cDNA后分别设计引物对Cecropin 2、Cecropin 3和Cecropin 4基因进行 PCR 扩增,分别得到带有双酶切位点的目的基因片段.将目的基因片段与 PMD18 T 载体连接,转化Top10克隆菌,测序正确后克隆入 pET28a 表达载体,进行双酶切和测序鉴定.研究结果表明,克隆得到的Cecropin p2、 Cecropin p3和Cecropin p4基因均由225个碱基组成,一个编码由74个氨基酸残基组成的开放阅读框,其蛋白相对分子质量约为8 kD,等电点分别为9.86、10.16和9.16.同源性分析结果显示:克隆所得猪蛔虫Cecropin p2、 Cecropin p3和Cecropin p4与数据库NCBI中的猪蛔虫Cecropin p2、Cecropin p3和Cecropin p4基因同源性均为100;,与其它Cecropin p基因同源性也较高(>42;).分子进化树显示, Cecropin p4的分化出现最早,其次为Cecropin p2, Cecropin p1和Cecropin p3分化得最晚.成功克隆了猪蛔虫Cecropin 2、Cecropin 3和Cecropin 4基因,构建了它们的原核表达载体并进行了生物信息学分析,为其重组表达和抑菌活性鉴定等研究奠定了理论基础. 相似文献
22.
为了平衡测验中限制条件而提出最大优先级指标(MPI)一阶段选题方法可能出现越界,对该情况进行分析,并给出修正方案,修正后基本解决了限制条件越界的问题. 相似文献
23.
罗氏沼虾原肌球蛋白基因的克隆表达及变应原性鉴定 总被引:2,自引:1,他引:1
通过NCBI查找到罗氏沼虾原肌球蛋白的mRNA,根据其CDS区域设计特异引物,通过反转录聚合酶链反应(RT-PCR)克隆出目的基因片段,测序后将该片段克隆到原核表达载体 pET-28a 上,转化到 E. coli BL21(DE3)和E. coli Rosatta后,经异丙基-B-D-硫代乳糖苷(IPTG)诱导表达,用Ni2+亲和层析柱对重组变应原进行纯化.采用免疫印迹检测其与对虾过敏患者血清的 IgE 结合活性.对罗氏沼虾变应原进行了表达、鉴定及纯化,成功地获得了具有变应原活性的重组罗氏沼虾原肌球蛋白,为罗氏沼虾过敏性疾病的诊断、治疗奠定了基础. 相似文献
24.
Dong Mingkai male years old master of technology lecture. * Supported by the High Technology Research Deverlopment program of China 《西南师范大学学报(自然科学版)》1998,(5)
Extensionisthemostimportantconceptindefaultlogic.Reiterhadmadesomeresearchonthespecialkindofdefaultlogic,thatisnormaldefaultl... 相似文献
25.
He Ping male years old professor. * Supported by the Netherlands Organization for Scientific Research NWO 《西南师范大学学报(自然科学版)》1998,(5)
ThegenusFusaeawasfoundedbySaford[1]in1914.ThetypespeciesF.longifoliawasoriginalydescribedbyAublet[2]asAnnonalongifolia.Bailo... 相似文献
26.
DONG LingYi JIANG KeWei ZHANG YanBin ZHANG Hui ZHUO HongQing CUI ZhiRong YE YingJiang * & WANG Shan * Department of Gastroenterological Surgery Surgical Oncology Laboratory Peking University People’s Hospital Peking University Beijing China 《科学通报(英文版)》2011,56(23):2444-2449
We previously showed that B cell receptor associated protein 31(BAP31) was significantly upregulated in colorectal cancer compared with normal mucosa epithelia. However, its expression pattern and pathological role in colorectal cancer are not clearly understood. In this study, we investigated whether the expression of BAP31 was associated with the clinicopathological parameters of colorectal cancer. The expression pattern of BAP31 was detected by immunohistochemistry on a tissue microarray in both primary ... 相似文献
27.
由于多元复合函数求导重要,难度大。而现在大学里通用的高等数学教材讲解的求导方法不是非常得通俗易懂,本文给出一个简单易懂的方法———画图法,解决多元复合函数求导问题。 相似文献
28.
YAN ZhenLin XIE WenJun GENG DeLu & WEI BingBo * Department of Applied Physics Northwestern Polytechnical University Xi’an China 《科学通报(英文版)》2011,56(31):3284-3288
We report on the ninth-mode sectorial oscillation of acoustically levitated drops excited by actively modulating sound pressure. A numerical computation based on the level set method was performed to model drop shape evolution by solving an incompressible two-phase flow problem. The calculated shapes of the oscillating drop are in good agreement with experimental observations. The relationship between the oscillation frequency and parameters describing the flattened drops is studied both experimentally and numerically. The frequency of the ninth-mode sectorial oscillation decreases with increasing equatorial radius and can be well-described by a modified Rayleigh equation. 相似文献
29.
Li Yuanfang female years old instructor. * This program has received the supports of the National Natural Science Foundation of China 《西南师范大学学报(自然科学版)》1998,(5)
Thestudyofthesupramolecularrecognitionoforganicdyeswithbiologicalmacromoleculeshasbecomeanincreasinglyinterestedresearchfield... 相似文献
30.
Interactions of pyrogallol red with proteins and the determination of proteins by resonance light-scattering 总被引:2,自引:0,他引:2
Hu Xiaoli female years old instructor. * Supported by the National Natural Science Foundation of China 《西南师范大学学报(自然科学版)》1998,(5)
Itisveryimportantthatthedeterminationofproteinconcentrationsinclinicaltestsandlaboratorypractice.Uptonow,therehavebeenmanyme... 相似文献