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61.
木质素降解菌L1原生质体的形成和再生 总被引:4,自引:1,他引:4
从自然界筛选出一株降解木质素高的白腐真菌 L1,对其原生质体的形成和再生进行了研究。在 OS培养基中生长的菌丝体 ,原生质体数量较高。在液体培养条件下 ,于 OS培养基中培养 60 h的菌丝体 ,用 0 .3% β-巯基乙醇与酶液同时处理菌丝体 ,采用 p H5 .0的混合酶 (蜗牛酶∶纤维素酶∶溶菌酶的最佳浓度比为 5∶ 4∶ 1 ) ;在 30℃酶解 4h;用 0 .4mol/L NH4Cl,1 0 mmol/L Mg SO4作渗透压稳定剂时 ,原生质体数量达到 4.32× 1 0 5个 /mg。 OS双层再生培养基最适于原生质体再生 相似文献
62.
真核原核两界细胞融合子SEM形态 总被引:2,自引:0,他引:2
报道应用扫描电子显微镜(SEM)、测定跨界融合Fhhh细胞形态的结果。Fhhh由2株真菌真核细胞与1株细菌原核细胞的原生质体融合而成。2株真菌是黄孢原毛平革菌和酿酒酵母,1株细菌是从对苯二甲酸废水活性污泥中筛选获得的土细菌。3个亲株菌体细胞SEM的形态差异极为显,既不同于黄孢原毛平革菌与土细胞的首次跨界融合获得的Fhh,也不同于Fhh与酿酒酵母的2次跨界融合获得的Fhhh。经过350多代的繁殖传代。Fhhh仍然同时含有来自3亲株的基因DNA,具有分子遗传稳定性。研究结果表明,跨界原生质体融合是一种快速有效的构建基因工程菌的生物技术。 相似文献
63.
We have studied the effects of two modulations — streptozotocin-induced diabetes in vivo, and the presence of the carboxylic proton ionophore monensin in vitro — on the degradation of3H-asialoorosomucoid ligand in isolated rat hepatocytes.The ligand was internalized by means of a synchronous wave procedure. Diabetes was associated with a marked decrease in the amount of total degraded radioactive ligand compared to that in normal cells (3.6% and 37.3% of internalized ligand respectively, at 60 min), together with increased secretion of degradation products into the incubation medium (87% and 46.3% of the total degraded ligand was secreted by diabetic and normal cells, respectively). Monensin induced similar effects in normal cells, but had no apparent effect in diabetic cells. 相似文献
64.
以四季樱草(Primula obconica),矮牵牛(Petunia hybrida)及黄花烟草(Nicotiana rustical)叶片为材料,用纤维素酶液分离原生质体,分离结果:三种植物叶肉原生质体数量,随酶液浸泡时间增长和浓度升高而增多;在同一浓度,同一时间酶解下,三种植物原生质体数量,以矮牵牛酶解数量最多,质量最好,四季樱草次之,黄花烟草较差。 相似文献
65.
血小板生成素(throm bopoietin, TPO)是调节血小板生成最主要的细胞因子,其生物学效应由其受体c-Mpl介导.利用酵母双杂合系统(tw o-hybrid system )筛选与c-Mpl相互作用的蛋白质因子,以Gal4 BD融合c-Mpl膜内部分cDNA 的pASMM 为靶蛋白质粒,筛选了人胎盘cDNA 文库,分离到人波形纤维蛋白(vim entin)的部分编码序列,首次检测到波形纤维蛋白与TPO 受体之间的相互作用,这提示细胞骨架蛋白可能在TPO 的信号转导过程中起着重要的作用 相似文献
66.
Apolipoprotein E (apoE) ɛ4 allele is a genetic risk factor for late-onset familial and sporadic Alzheimer’s disease (AD).
In the central nervous system, apoE is secreted mainly by astrocytes as a constituent of high-density lipoproteins. A recent
study using apoE knockout mice provided strong evidence that apoE promotes cerebral deposition of amyloid β protein (Aβ).
However, no clear explanation of the pathogenesis of apoE-induced AD has been provided. Here we discuss two possible mechanisms
by which apoE might enhance Aβ deposition. One is the intracellular pathway in which apoE is internalized by neurons and induces
lysosomal accumulation of Aβ and amyloidogenic APP (amyloid precursor protein) fragments, leading to neuronal death. The other
is the extracellular pathway in which apoE-containing lipoproteins are trapped by Aβ1–42 deposits mobilizing soluble Aβ peptides
and consequently enlarge amyloid plaques. These two mechanisms may operate at different stages of AD pathogenesis and suggest
a chaperone-like function for the apoE molecule.
Received 4 February 1999; received after revision 9 April 1999; accepted 23 April 1999 相似文献
67.
Our understanding of the mode of action of parathyroid hormone-related protein (PTHrP) has changed profoundly during the last decade. Most PTHrP activities are mediated by membrane receptors through autocrine/paracrine pathways. However, both endogenous and exogenous PTHrP also appear to have intracrine effects through translocation into the nucleus. The present review proposes unconventional PTHrP signalling, based on novel clues. First, PTHrP binding to its membrane receptor triggers internalization of the whole complex, mediated by beta-arrestin. There is growing evidence that the receptor and arrestin are the effectors of biological responses, rather than the ligand (or in addition to the ligand). Second, the existence of putative PTHrP targets within the cytoplasm is beginning to be supported. Recent findings of interactions between a COOH-terminus of PTHrP and beta-arrestin and between the PTHrP receptor and 14-3-3 proteins represent the starting point for identification of intracellular partners of both the hormone and its receptor.Received 19 June 2003; received after revision 10 July 2003; accepted 21 July 2003 相似文献
68.
Davoli C Marconi A Serafino A Iannoni C Marcheggiano A Ravagnan G 《Cellular and molecular life sciences : CMLS》2002,59(3):527-539
Nerve growth factor (NGF) belongs by sequence homology to the neurotrophins, a family of proteins binding the same p75 receptor
and closely related members of the Trk family of receptor tyrosine kinases. Fundamental in the vertebrate nervous system,
neurotrophin signals have also been suggested as essential for relatively complex nervous systems occurring in invertebrate
species that live longer than Caenorhabditis elegans and Drosophila melanogaster. Mammalian neurotrophins have been found to influence invertebrate neuronal growth. However, there are only a few data on
the presence of molecules related to neurotrophin signalling components in invertebrates. Our studies provide evidence that
analogues of neurotrophins and neurotrophin receptors are expressed in Eisenia foetida earthworms. In particular, NGF-like and Trk-like immunoreactive proteins are both expressed in the nervous system, whereas
p75-like positivity identifies tubular structures associated with dorsal pores that are involved in the earthworm response
to mechanical irritation or stress.
Received 12 November 2001; received after revision 8 January 2002; accepted 8 January 2002 相似文献
69.
Enzymes and receptors in the leukotriene cascade 总被引:7,自引:0,他引:7
Leukotrienes are a family of paracrine hormones derived from the oxidative metabolism of arachidonic acid. These lipid mediators
are recognized as important signal molecules in a variety of inflammatory and allergic conditions affecting the skin, joints,
gastrointestinal and respiratory systems, in particular asthma. Such conditions are typified by local pain, tissue edema,
hyperemia and functional losses. In the tissues, immunocompetent cells accumulate at the site of injury which contribute to
tissue damage and perpetuation of the disease process. Leukotrienes can elicit most, if not all, of these signs and symptoms.
Thus, leukotriene B4 is one of the most powerful chemotactic agents known to date and participates in the recruitment of leukocytes. The cysteinyl
leukotrienes, on the other hand, contract smooth muscles, particularly in the peripheral airways and microcirculation. Recently,
drugs which block the formation and action of leukotrienes have been introduced as novel antiasthmatic medications. This chapter
reviews the biochemistry, molecular biology and cell biology of the key enzymes and cognate receptors in the leukotriene cascade. 相似文献
70.
Clustering of neurotransmitter receptors in the postsynaptic membrane is critical for efficient synaptic transmission. During
neuromuscular synaptogenesis, clustering of acetylcholine receptors (AChRs) is an early sign of postsynaptic differentiation.
Recent studies have revealed that the earliest AChR clusters can form in the muscle independent of motorneurons. Neurally
released agrin, acting through the muscle-specific kinase MuSK and rapsyn, then causes further clustering and localization
of clusters underneath the nerve terminal. AChRs themselves are required for agrin-induced clustering of several postsynaptic
proteins, most notably rapsyn. Once formed, AChR clusters are stabilized by several tyrosine kinases and by components of
the dystrophin/utrophin glycoprotein complex, some of which also direct postnatal synaptic maturation such as formation of
postjunctional folds. This review summarizes these recent results about AChR clustering, which indicate that early clustering
can occur in the absence of nerves, that AChRs play an active role in the clustering process and that partly different mechanisms
direct formation versus stabilization of AChR clusters.
Received 10 April 2002; received after revision 4 June 2002; accepted 10 June 2002 相似文献