硝基烷烃互变异构的量子化学研究

用ＭＮＤＯ方法研究了硝基烷烃分子中α－Ｈ原子的［１，３］迁移反应，用Ｐｕｌａｙ方法优化了反应物和产物的平衡几何构型，算得了反应途径，获得了过渡态，由虚振动模式对过渡态进行了确证。计算了该反应的热力学参量，进一步谁了该反应发生的可能性及和反应历程。     

不依赖特异性抗体激活补体经典途径的机理探讨

实验证实了Ｅ．ｃｏｌｉＢ和Ｅ．ｃｏｌｉｋ１２ ｇａｌ＾＋的细胞壁可直接激活补本，去壁扣的细菌球形体或原生质体加入补体后可裂解，说明在不依赖特异抗体激活补体经典途径中，补体的激活部位是细胞壁，作用靶子似乎是细胞膜。     

Br+C2H4→CH2CHBr+H的从头计算研究

用MP2方法，6-311＋G（d，p）基组，研究了反应Br＋GH4→CH2CHBr＋H的可能反应路径和过渡态以及中间体CH2BrCH2的分解过程．给出了最可能的反应路径及相应的反应物、过渡态和产物零点能修正后的能量．     

肝再生

肝脏在受到损害或切除之后,可以显示出强大的再生能力。肝再生这一现象早已被人们发现并研究。肝脏再生的启动因素有哪些,又是如何适时终止的?在肝再生过程中,哪些基因的表达发生了变化?哪些信号通路参与了肝再生过程?这些问题一直是研究者关注的焦点。本文将对肝再生研究的这些热点问题进行综述。     

高山红景天酪醇生物合成途径的探讨

酪醇作为高山红景天的主要活性成分之一,其生物合成途径尚不清楚.笔者对其生物合成途径作了分析和讨论.     

Effects of ubiquitin-protea-some pathway on mouse sperm capacitation, acrosome reaction and in vitro fertilization

Chlortetracycline (CTC) fluorescence patterns were used to study changes in the patterns B and AR of mouse sperm after incubation with reagents that would block the UPP. They were the monoclonal antibody against ubiquitinated proteins-UCP1; the polyclonal antibody against ubiquitin anti-Ub, and a special inhibitor against proteasome ALLN. Furthermore, we treated the capacitated sperm or the eggs with these reagents separately and tested whether the normal in vitro fertilization was blocked or not. Results illustrate that UCP1, anti-Ub, and ALLN have little effects on sperm capacitation and acrosome reaction,but they do inhibit fusion of mouse sperm with eggs, which suggests that UPP play an important role in mouse in vitro fertilization.``     

代谢工程和作物改良

基因组学和植物生理生化是通过基因工程改良作物的基础 .借助于这些基础研究 ,通过基因工程调控代谢途径 ,提高了油菜籽粒中的油脂含量和拟南芥种子中的维生素 E活性 ,使水稻的胚乳中能合成维生素A原 ,增加了水稻在碱性土壤中吸收铁的能力 ,展示了代谢工程在定向改良作物中的应用前景     

Isoprenylated proteins

Isoprenoids are synthesized in all living organisms and are incorporated into diverse classes of end-products that participate in a multitude of cellular processes relating to cell growth, differentiation, cytoskeletal function and vesicle trafficking. In humans, the non-sterol isoprenoids, farnesyl pyrophosphate and geranylgeranyl-pyrophosphate, are synthesized via the mevalonate pathway and are covalently added to members of the small G protein superfamily. Isoprenylated proteins have key roles in membrane attachment and protein functionality, have been shown to have a central role in some cancers and are likely also to be involved in the pathogenesis and progression of atherosclerosis and Alzheimer disease. This review details current knowledge on the biosynthesis of isoprenoids, their incorporation into proteins by the process known as prenylation and the complex regulatory network that controls these proteins. An improved understanding of these processe is likely to lead to the development of novel therapies that will have important implications for human health and disease. Received 5 July 2005; received after revision 17 October 2005; accepted 22 October 2005     

Role of FGF-2／FGFR signaling pathway in cancer and its signification in breast cancer

Fibroblast growth factor-2 (FGF-2) is a member of a large family of proteins that bind heparin and heparan sulfate and modulate the function of a wide range of cell types. It has been proved that FGF-2 stimulates the growth and development of new blood vessels (angiogenesis) that contribute to the pathogenesis of several diseases (i.e. cancer, atherosclerosis). However, many of the biological activities of FGF-2 have been found to depend on its receptor抯 intrinsic tyrosine kinase activity and second messengers such as the mitogen activated protein kinases. This review will focus on the mechanism of FGF-2/FGFR induced signaling pathway in tumor and human breast cancer.     

花粉管通道法转基因技术在甜瓜品种河套蜜瓜上的应用

为建立甜瓜(Cucumis melo L.)简便易行转化频率高的转基因技术,以甜瓜品种河套蜜瓜为受体材料,以含gus基因的植物双元表达载体pPZP221为外源基因供体,进行了花粉管通道法转基因研究.自交授粉后分别于1、2、3、4、5、6、7、8、9、10 h,切去柱头上端1/3部分,立即滴加质粒DNA溶液,果实成熟后收获转化种子.对T0代植株进行PCR检测,结果表明不同时间处理所得到的T0代植株均具有较高的转化频率,其中授粉后7 h滴加DNA溶液所获得的转化率为28.3%.对一部分PCR阳性的T0代植株基因组DNA进行Southern杂交分析,结果表明所有样品均出现特异性杂交条带,证明外源基因已整合到受体植物基因组中.