Spontaneous Neurotransmitter Release Depends on Intracellular Rather than ER Calcium Stores in Cultured Xenopus NMJ

Introduction During development, many cell types exhibit sponta-neous neurotransmitter release, with synaptic transmis-sions crucial for normal nervous system activity. Syn-aptic transmissions are initiated when an action poten-tial triggers the neurotran…     

微波辅助磁记录技术研究

颗粒磁矩反转场的大小和反转速度是衡量磁记录写入效率的主要指标.利用LL方程描述在微波场下颗粒磁矩的动力学行为,考虑了颗粒磁矩各向异性场之间存在一定夹角,计算了颗粒磁矩之间相互作用对反转场大小以及反转速度的影响,对于单个颗粒磁矩提出了能够有效提高磁矩反转速度,同时降低磁矩反转场的方法.     

Amyloid precursor protein and its homologues: a family of proteolysis-dependent receptors

The Alzheimer’s amyloid precursor protein (APP) belongs to a conserved gene family that also includes the mammalian APLP1 and APLP2, the Drosophila APPL, and the C. elegans APL-1. The biological function of APP is still not fully clear. However, it is known that the APP family proteins have redundant and partly overlapping functions, which demonstrates the importance of studying all APP family members to gain a more complete picture. When APP was first cloned, it was speculated that it could function as a receptor. This theory has been further substantiated by studies showing that APP and its homologues bind both extracellular ligands and intracellular adaptor proteins. The APP family proteins undergo regulated intramembrane proteolysis (RIP), generating secreted and cytoplasmic fragments that have been ascribed different functions. In this review, we will discuss the APP family with focus on biological functions, binding partners, and regulated processing.     

Functions of reticulons in plants: What we can learn from animals and yeasts

Reticulons (RTNs) are membrane-spanning proteins sharing a typical domain named reticulon homology domain (RHD). RTN genes have been identified in all eukaryotic organisms examined so far, and the corresponding proteins have been found predominantly associated to the endoplasmic reticulum membranes. In animal and yeast, in which knowledge of the protein family is more advanced, RTNs are involved in numerous cellular processes such as apoptosis, cell division and intracellular trafficking. Up to now, a little attention has been paid to their plant counterparts, i.e., RTNLBs. In this review, we summarize the data available for RTNLB proteins and, using the data obtained with animal and yeast models, several functions for RTNLBs in plant cells are proposed and discussed. Received 01 July 2008; received after revision 08 September 2008; accepted 30 September 2008     

大鼠背根神经节分离细胞离子单通道记录技术

本文探讨了在大鼠背根神经节(DRG)细胞上记录离子单通道电流的方法,研究了膜片钳实验的各个环节,研究了提高封接电阻的方法。研究结果表明,单通道记录的背景噪声取决于电极与细胞的封接水平。文中还讨论了实验系统的组装,电极制备和细胞分离等技术。     

大型田径运动会计算机管理系统的探讨

为了对国际或全国性大型比赛进行科学管理,必须建立一个功能完善、自动化程度高的计算机管理系统。本文在总结第六届全运会田径比赛首次全面使用计算机管理系统的基础上,提出改进和完善其功能的一些设想和建议,并探讨了大型田径运动会使用计算机进行编排记录公告的工作方法。     

光盘系统的脉冲响应函数

以信息通道的写读脉冲响应概念为基础，建立了光盘数据存储系统的模型，得到了脉冲响应函数和频率传递函数，分析了光学头的伺服误差和像差对读出过程的影响．     

Fe含量对FePt磁性薄膜的微结构和磁特性的影响

用直流磁控溅射方法和原位退火工艺在玻璃基片上制备了FexPt100-x纳米颗粒膜.研究发现,Fe含量对FePt纳米颗粒膜的微结构和磁特性有很大的影响.矫顽力随Fe含量的增加而增大,当x=48时矫顽力Hc达到了1 040 kA/m,样品出现很好的有序化L10结构扫描探针显微镜(SPM)观察结果显示,所有样品具有横跨数个晶粒的粒状磁畴,Fe48Pt52的粗糙度Ra大约0.6 nm.     

Sb-Se系和Ge-Sb-Te系相变光盘记录介质微观结构及光学性能研究

利用X射线衍射仪 ,分光光度计对Sb Se系和Ge Sb Te系相变光盘记录介质材料非晶态薄膜相变前后结构的变化 ,光学性能进行了系统的研究 ,X射线衍射分析表明 :SbSe非晶态薄膜退火后有Sb的析晶峰 ,SbSe2 有Se的析晶峰 ,符合化学计量比的Sb2 Se3 全部是Sb2 Se3 的共晶峰 .GeSb2 Te4 非晶态薄膜在热退火过仇逐首先形成fcc亚稳相 ,升高退火温度 ,Fcc相转变为稳定的hex相 ,GeSb4 Te4 非晶态薄膜退火后在发生上述变化的同时 ,还有Sb的析晶峰 .分光光度计测试表明 :Sb Se系非晶态的光稳定性很不理想 ,随着波长的改变 ,反射率变化太快 .对于Ge Sb Te系合金 ,在各种波段处 ,两种合金都有较大的反衬度 ,其非晶态的光稳定性也较理想 ,随着波长的改变 ,反射率变化不大     

Research at channel level on the effect of LaCl3 on Radish (Raphanus satirus L.) vacuolar membrane

We recorded slow vacuolar (SV-type) channel currents of Radish vacuoles successfully for the first time by using the whole-vacuolar patch-clamp recording mode. SVtrye currents would increase and threshold potentials of activation would shift towards more negative values with the increase of concentrations of cytosolic Ca2+. When 2.5 mmol/L LaCl3 and 4 mmol/L EGTA were added to bath solutions,SV-type currents were suppressed remarkably. Then adding LaCl3 with different concentrations to pipette solutions, we found that LaCl3 with higher concentrations (＞ 4 × 10-7 mol/L)had a strong inhibitory effect on SV-type currents, while LaCl3 with lower concentrations (≤4 × 10-7 mol/L) promoted channel currents. This promoting effect provides an important basis at channel level for researching further the effects of rare earth on physiological activities of plants and the production-increase effects of rare earth fertilizers on crops.``