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971.
NIH3T3细胞以劳氏肉瘤病毒(RSV)经温度敏感突变株转染形成的细胞株tsLA90,细胞在允许温度(40℃)时为正常表型,在允许温度(33℃)下为转化表型。40℃时,微丝分布发达,从40℃移至33℃1h,微丝完全解聚,同时,其外基质成分纤粘蛋白(FN)亦相伴解聚。若在40℃以外源性FN或对蛋白激酶C(PKC)具有抑制作用的三氟拉嗪进行预处理后,再转入33℃时便能抑制微丝的解聚。结果提示,除RSV 相似文献
972.
LI Zesong CAI Shaoxi JIANG Yua GUO RuiJun ZHANG Wen 《武汉大学学报:自然科学英文版》2006,11(3):720-724
Cultured HSCs were treated colchicine with different concentrations for 12 h, respectively. The effects of eolehicine on HSCs growth were measured by MTT assay. Effects of eolchicine on gene expression of HSCs were analysed by using a self-made oligonucleotide mieroarray. Colehieine inhibited HSCs growth in a dose-dependent manner. After 12 h of treatment with 6.25 mg/L of colehicine, the expression of tissue inhibitor of metalloprotenase-1 (TIMP-1) and the expression of interleukin-6 (IL-6) in HSCs were downregulated by 2.3 folds and 2.1 folds, respectively. These results suggest that colchieine's beneficial effects may, at least in part, owe to the inhibitory to the proliferation of HSCs and down regulation of the expression of both TIMP1 and IL-6 in HSCs. 相似文献
973.
本文利用测量电池电动势的方法,求出了溶解在冰晶石熔体中的Al_2O_3(饱和)的平衡分解电压(E°T),并依此算出了其标准生成自由能变化(△G°T),将实测结果与理论计算做了对比,得出偏差为0.4—0.9%。 相似文献
974.
Summary Ciliation in endometrial fibroblasts and myometrial muscle cells of the rat was examined by transmission electron microscopy. Quantification of the number of ciliated cells during the estrus cycle did not show any firm relationship between cilation and ovarian hormonal activity. In the case of most cilia, there is a spatial relationship between their basal centrioles and the Golgi complex, so that a Golgi-cilium complex is created. A possible role of ciliation in uterine fibroblasts and smooth muscle cells is discussed. 相似文献
975.
V. Hoftiezer P. W. Bankston P.-O. Berggren 《Cellular and molecular life sciences : CMLS》1987,43(4):420-421
Summary The ultrastructural morphology of the clonal insulin-producing cell line, RINm5F, was investigated. Virus-like particles, probably C type viruses, were identified both intra- and extracellularly. Because these particles could not be found in the original transplantable tumor, it is probable that viruses were induced at some later stage in the development of the RINm5F cell line. All investigators using the RINm5F cells should be aware of the fact that these cells may contain one or several types of viruses, and of the possibility that these particles may interfere with a variety of cellular functions.This work was supported by grants from the Lake County Medical Center Developmental Agency (to VH and PWB) and the American Heart Association, Indiana Affiliate (to PWB,*USA) and by the Swedish Medical Research Council (12X-562), the Swedish Diabetes Association, the Nordic Insulin Foundation and Åke Wibergs Foundation (**Sweden). Per-Olof Berggren is a recipient of a postdoctoral fellowship from the Swedish Medical Research Council. 相似文献
976.
研究了双环戊二烯基铁作为燃料添加剂的应用、实验表明:双环戊二烯基铁加入煤和柴油等燃料后,可使燃料燃烧更加充分.燃烧热有大幅度地提高、同时燃烧速率加快、而且可以抑制煤里面硫的燃烧,使二氧化硫的生成量降低.当燃料与双环戊二烯基铁的质量之比为500:1时,上述效果最为明显. 相似文献
977.
张纯祥 《中山大学学报(自然科学版)》1995,(1)
细胞辐射损伤的径迹结构模型给出了V-79中国仓鼠细胞的重离子失活截面与实验相符合的结果,井明显地表现出“径迹宽度区”所特有的thindown现象,计算用的放射灵敏参数是由存活曲线得到的,径向剂量分布用数值积分方法计算。本文的结果与不考虑δ射线发射角的径向剂量分布公式相比,有较大改进,重离子失活戳面与实验符合得更好。 相似文献
978.
Principles of neural cell migration 总被引:19,自引:0,他引:19
P. Rakic 《Cellular and molecular life sciences : CMLS》1990,46(9):882-891
Summary A basic property of immature neurons is their ability to change position from the place of their final mitotic division in proliferative centers of the developing brain to the specific positions they will occupy in a given structure of the adult nervous system. Proper acquisition of neuron position, attained through the process of active migration, ultimately affects a cell's morphology, synaptic connectivity and function. Although various classes of neurons may use different molecular cues to guide their migration to distant structures, a surface-mediated interaction between neighboring cells is considered essential for all types of migration. Disturbance of this cell-cell interaction may be important in several congenital and/or acquired brain abnormalities. The present article considers the basic mechanisms and principles of neuronal cell migration in the mammalian central nervous system. 相似文献
979.
980.
Development of the CAS-LIBB single-particlemicrobeam for localizedirradiation of living cells 总被引:3,自引:0,他引:3
《科学通报(英文版)》2004,49(17)
The use of microirradiation techniques in radiationbiology dates back to the work of Zirkle and his col-leagues in 1950’s[1,2]. However, biological effects of arandom single particle traversal in living materials re-mained highly unknown since they could not be simulatedby conventional broad-field radiation exposures. In recentyears, there has been a resurgence of interest in sin-gle-particle/single-cell microbeams[3—12]. The biologicalinterest in the single-particle microbeam stems from the… 相似文献