| Colchicine Inhibited the Expression of Tissue Inhibitor of Metalloprotenase-1 and Interleukin-6 in Cultured Activated Hepatic Stellate Cells |
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| 作者姓名： | LI Zesong CAI Shaoxi JIANG Yua GUO RuiJun ZHANG Wen |
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| 作者单位： | [1]College of Bioengineering, Chongqing University, Chongqing 400044,China [2]不详, Chongqing University, Chongqing 400044,China [3]Shenzhen Yishengtang Biological Products Co. lad, Shenzhen 518054,Guangdong, China [4]Shenzhen Shajing People＇s Hospital, Shenzhen 518104, Guangdong,China |
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| 基金项目： | Supported by a Grant from the Seience and Technology Committee of Guangdong Province （2003B31801） |
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| 摘 要： | Cultured HSCs were treated colchicine with different concentrations for 12 h, respectively. The effects of eolehicine on HSCs growth were measured by MTT assay. Effects of eolchicine on gene expression of HSCs were analysed by using a self-made oligonucleotide mieroarray. Colehieine inhibited HSCs growth in a dose-dependent manner. After 12 h of treatment with 6.25 mg/L of colehicine, the expression of tissue inhibitor of metalloprotenase-1 （TIMP-1） and the expression of interleukin-6 （IL-6） in HSCs were downregulated by 2.3 folds and 2.1 folds, respectively. These results suggest that colchieine＇s beneficial effects may, at least in part, owe to the inhibitory to the proliferation of HSCs and down regulation of the expression of both TIMP1 and IL-6 in HSCs.
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| 关 键 词： | 秋水仙碱 组织抑制剂 肝纤维症 肝组织 白细胞间素6 |
| 文章编号： | 1007-1202（2006）03-0720-05 |
| 收稿时间： | 2005-06-22 |
Colchicine inhibited the expression of tissue inhibitor of metalloprotenase-1 and interleukin-6 in cultured activated hepatic stellate cells |
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| LI Zesong CAI Shaoxi JIANG Yua GUO RuiJun ZHANG Wen.Colchicine Inhibited the Expression of Tissue Inhibitor of Metalloprotenase-1 and Interleukin-6 in Cultured Activated Hepatic Stellate Cells[J].Wuhan University Journal of Natural Sciences,2006,11(3):720-724. |
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| Authors: | Li Zesong Cai Shaoxi Jiang Yuan Guo RuiJun Zhang Wen |
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| Institution: | (1) College of Bioengineering, Chongqing University, 400044 Chongqing, China;(2) Shenzhen Yishengtang Biological Products Co. Ltd, 518054 Shenzhen, Guangdong, China;(3) Shenzhen Shajing People's Hospital, 518104 Shenzhen, Guangdong, China |
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| Abstract: | Cultured HSCs were treated colchicine with different concentrations for 12 h, respectively. The effects of colchicine on HSCs
growth were measured by MTT assay. Effects of colchicine on gene expression of HSCs were analysed by using a self-made oligonucleotide
microarray. Colchicine inhibited HSCs growth in a dose-dependent manner. After 12 h of treatment with 6.25 mg/L of colchicine,
the expression of tissue inhibitor of metalloprotenase-1 (TIMP-1) and the expression of interleukin-6 (IL-6) in HSCs were
down-regulated by 2.3 folds and 2.1 folds, respectively. These results suggest that colchicine's beneficial effects may, at
least in part, owe to the inhibitory to the proliferation of HSCs and down-regulation of the expression of both TIMP1 and
IL-6 in HSCs.
Foundation item: Supported by a Grant from the Science and Technology Committee of Guangdong Province (2003B31801)
Biography: LI Zesong (1968-), male, Associate Professor. Research direction: microarray, biochemical pharmacology, molecular diagnosis
of disease. |
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| Keywords: | microarray colchicines tissue inhibitor of metalloprotenase-1 liver fibrosis hepatic stellate cells interleukin-6 |
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