Human endothelial senescence induced by IL-1α in vitro

Interleukin 1(IL-1) is an important proinflammatory cytokine that causes pleiotropic effects. Vascular endothelial cells stimulated by IL-1α can lead to the inflammatory response. Reactive oxygen species (ROS) are also generated at the site of inflammation and serve as an important factor against foreign invader. Here we report that long-term stimulation of human vein endothelial cells with IL-1α can accelerate their senescence associated with β-galactosidase activity. The flow cytometric analyses showed that most of the induced cells entered G0-G1 phase. DNA damage was more severe in senescent cells by comet assay. The induced cells by IL-1α had higher levels of ROS and malonyldialdehyde (MDA), lower activity of antioxidant enzymes and lower capacity of total antioxidant systems than control, which led to cell damage and cell degeneration, that is to say, which contributed to cellular senescence. Our results gave a direct proof to a new hypothesis-"the inflammation hypothesis of aging" on cellular level, and also provided a basis for the study on anti-aging and aging-related diseases.     

复杂点阵对单晶体多波长衍射的影响

本文根据单晶体简单点阵劳厄衍射理论,利用衍射指数变换的方法,论证了复杂点阵对单晶体多波长衍射的影响导致某些衍射指数在衍射中消失,结果表明各种复杂点阵衍射指数消失的条件是C面底心点阵H与K的和为奇数;B面底心点阵L与H的和为奇数;A面底心点阵K与L的和为奇数;体心点阵H,K和L三者的和为奇数;面心点阵H,K和L奇偶混杂。     

银杏叶细胞的亚显微结构简报

用透射式电子显微镜对银杏叶片和叶柄细胞进行了观察,这些细胞的叶绿体大而多,片层结构发达;细胞核小而扁平,核质松散,内质网,高尔基体和微体则很少发现,并对此进行了分析。     

枯草杆菌原生质体再生前后细胞壁的变化及其对酶渗透性的影响

报道了枯草芽孢杆菌Bacillus subtilis BF—7658原生质体的制备、再生及其传代后的细胞的电镜观察;酶产量的变化;初步证明经原生质体再生所得到的α——淀粉酶高产菌株,同其细胞壁再生过程及细胞壁的渗透性有着密切关系.     

实际面间距的简捷计算方法

通过分析结晶学晶胞和固体物理原胞之间的异同点和相互联系，导出了一种计算面间距的简捷方法，该方法有效地综合了二者的优点，即可免去插入平面的麻烦，双可方便直接地求出实际面间距。     

圆环的有限剖分及其数据结构

对作为常用基本体素的圆环进行了空间单元及三角剖分的研究，给出了用空间单元法及三角剖分法表示圆环的原理和方法，同时也给出了该三维模型的数据结构，简化了算法，并从根本上为解决在圆环面上定义四维曲面及用离散法进行曲面求交等问题提供了保证     

再生障碍性贫血病人红细胞膜骨架蛋白的研究

十二烷基磺酸钠—聚丙烯酰胺凝胶电泳(简称为SDS-PAGE)方法分离12例再生障碍性贫血(简称再障)病人红细胞膜蛋白,发现其中有6例红细胞骨架蛋白带1、带2和带4有不同程度减少或缺损。同时用扫描电子显微镜观察其红细胞形态,发现亦有不同程度异常,表现为棘状、刺状及波浪状。提示红细胞骨架蛋白的含量与红细胞的形态有密切关系。     

烟草DH群体的构建及其性状表现

应用秋水仙碱混合二甲基亚砜的方法，对烟草单倍体进行加倍，采用气孔保卫细胞叶绿体计数法筛选加倍单倍体，成功构建烟草DH系；并对分别来自两个杂交组合的40个DH系的主要农艺性状进行了考察。