双向运输带式输送机的动力学设计

矿山掘进工作面,需要同时向工作面运送支护材料和向外运输采掘下来的岩石（或煤炭）,两者运行方向相反,同时使用输送带的上、下分支恰好解决这一问题。以这一上、下分支同时运输物料的双向运输带式输送机为例,对其建立了动力学计算模型。依据作用于滚筒上力的平衡方程式,给出了双向运输带式输送机启动加速度和制动减速度的计算公式;按照匀加减速转动,给出了加速和减速时间的计算公式;依据输送带作用于滚筒上的静张力和动张力,给出了输送带在滚筒上不打滑的条件;按照不打滑条件和垂度条件分别给出了输送带最小张力的计算公式;依据托辊不圆造成对输送带的扰动,给出了激励频率的计算公式;依据弹性体动力学,给出了输送机的固有振动频率和输送带最大弹性变形量的计算公式,并附有设计计算实例。     

GCC box in<Emphasis Type="Italic">Arabidopsis PDF1.2</Emphasis> promoter is an essential and sufficient cis-acting element in response to MeJA treatment

The expression of Arabidopsis PDF1.2 gene isregulated by jasmonic acid (JA) and ethylene (ET). It also has been well documented that GCC box is an element responsive to ET, however, the responsive mechanism of JA in such plant defense gene expression is unclear. In this paper, the authors define the essential cis-acting element in PDF1.2 promoter responsive to methyl jasmonate (MeJA) through fragment deletions and site-directed mutageneses combiningAgrobacterium-mediated transient reporter gene expression in tobacco leaves. Firstly, the MeJA inducible expression o fPDF1.2 was confirmed by using the upstream -1.86 kb fragment of PDFI.2 gene. Secondly, the upstream -300— -243 bp fragment of the promoter was evidenced to respond to MeJA. To further characterize this promoter region, three point mutations were introduced into the -300— -243 bp fragment of the promoter. This result showed that the mutation of GCC box abolished MeJA induction, whereas the mutations of the G box-like and the imperfect palindrome sequence did not significantly decrease MeJA inducible effect, indicating that GCC box in PDFI.2 is essential for MeJA induction. The sufficient responsiveness to MeJA of this GCC box was further investigated by 4xGCC fused upstream to the CaMV 35S minimal promoter. This result suggested that the fused promoter was able to activate reporter gene expression in response to MeJA. Thus these results indicate that the GCC box in PDFI.2 is an essential and sufficient element to confer MeJA induction.     

Stability analysis of BAM neural networks with time-varying delays

Some new criteria for the global asymptotic stability of the equilibrium point for the bi-directional associative memory neural networks with time varying delays are presented. The obtained results present the structure of linear matrix inequality which can be solved efficiently. The comparison with some previously reported results in the literature demonstrates that the results in this paper provide one more set of criteria for determining the stability of the bi-directional associative memory neural networks with delays.     

Structural analysis of the promoter of tomato 1-aminocyclopropane-1-carboxylate synthase 6 gene(Le-ACS6)

Ethylene plays an important role in the regulation of many growth and developmental processes of higher plants. In tomato,Le-ACS6,a member of the ACC synthase multigene family involved in system 1 ethylene biosynthesis during fruit ripening,is subject to negative feedback regulation by ethylene. To identify the cis-elements that are responsible for the negative feedback control,we established an in vitro transient assay system employing particle bombardment on mature-green tomato fruit pericarp to examine the expression of a luciferase(LUC) reporter gene driven by a 5′-serially deleted Le-ACS6 promoter. The results localized putative cis-elements required for negative ethylene-response between -347 and -266 upstream from the translational start site ATG. Several lines of stable transformation of the Le-ACS6 promoter and GUS reporter fusion gene containing internal deletion from -347 to -266 were generated. The expression pattern of the GUS reporter showed that removal of the nucleotides from -347 to -266 completely eliminated the response of the Le-ACS6 promoter to exogenous ethylene.     

水稻16kDa醇溶蛋白启动子克隆及载体构建

水稻种子16 kDa醇溶蛋白是水稻种子成熟过程中,由16 kDa基因编码,16 kDa醇溶蛋白启动子调控,在胚乳中特异表达的蛋白质.以水稻基因组DNA为模板,通过PCR扩增技术得到16 kDa启动子片段,序列分析结果表明:获得的启动子片段的大小为931 bp,与已报道的该启动子序列相比较,其核苷酸序列同源性为99.9%.该启动子区域含有TATA-box,CAAT-box,GCN4基序,Prolamin-box等胚乳特异表达启动子所必需的正调控元件.利用该启动子构建了植物种子特异表达载体pC16 kDP.     

Stability analysis of BAM neural networks with time-varying delays

Some new criteria for the global asymptotic stability of the equilibrium point for the bi-directional associative memory neural networks with time varying delays are presented. The obtained results present the structure of linear matrix inequality which can be solved efficiently. The comparison with some previously reported results in the literature demonstrates that the results in this paper provide one more set of criteria for determining the stability of the bi-directional associative memory neural networks with delays.     

IL-1β regulates the mouse Fas ligand expression in corneal endothelial cells

Constitutively expressed Fas ligand (FasL) in several distinct epithelial cell types appears to protect tissues by inducing apoptosis of Fas immune cells during inflammatory reactions. To study the rela-tionship of FasL and inflammation process in cornea,we examined the effects of inflammatory cytokine IL-1β on the FasL production,expression and cytotoxic function in corneal endothelial cells. In this paper,we demonstrate that IL-1β inhibits the FasL production and expression in corneal endothelial cells. The promoter activities of FasL in these cells are reduced by IL-1β in a dose-dependent manner. Finally,we also find that IL-1β block the cytotoxic effects of FasL derived from corneal endothelial cells to the Fas target cells. These data support the view that FasL derived from corneal endothelial cells modulate inflammation within cornea.     

Cloning of aminopeptidase N promoter and its activity in hematopoietic cell and different tumor cell lines

Aminopeptidase N (APN) promoter region was cloned and sequenced from peripheral blood mononuclear cells. The recombinant reporter construct containing the promoter and luciferase gene, designated pXP1-APNLuc, was introduced into myeloblastic cell line, T lymphocyte cell line and various tumor cell lines. Luciferase assay showed that APN upstream promoter is myeloid-specific for high expression in myeloblastic cell line and much lower expression in T lymphocyte cell line. The promoter activity was relatively high in lung adenoma cell line compared with other tumor cell lines including hepatoma cell line, tong cancer cell line and esophageal cancer cell line in which the promoter activity significantly diminished or was almost undetectable. The characteristics of APN promoter may provide a new strategy for specific myeloprotection while tumor patients are being treated with chemotherapy and/or radiotherapy.     

基于BiTCNSA的恶意代码分类方法

当前恶意代码的对抗技术不断变化，恶意代码变种层出不穷，使恶意代码分类问题面临严峻挑战。针对目前基于深度学习的恶意代码分类方法提取特征不足和准确率低的问题，提出了基于双向时域卷积网络(BiTCN)和自注意力机制(Self-Attention)的恶意代码分类方法(BiTCNSA)。该方法融合恶意代码操作码特征和图像特征以展现不同的特征细节，增加特征多样性。构建BiTCN对融合特征进行处理，充分利用特征的前后依赖关系。引入自注意力机制对数据权值进行动态调整，进一步挖掘恶意代码内部数据间的关联性。在Kaggle数据集上对模型进行验证，实验结果表明:该方法准确率可达99.75%，具有较快的收敛速度和较低的误差。