| 水稻16kDa醇溶蛋白启动子克隆及载体构建 |
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| 引用本文： | 王景雪,杨翠平,梁超,刘莉.水稻16kDa醇溶蛋白启动子克隆及载体构建[J].山西大学学报(自然科学版),2007,30(2):265-269. |
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| 作者姓名： | 王景雪 杨翠平 梁超 刘莉 |
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| 作者单位： | 1. 山西大学生命科学与技术学院,山西,太原,030006
2. 中山大学生命科学学院,有害生物控制及资源利用国家重点实验室,广州,510275 |
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| 基金项目： | 山西省太原市科技启明星计划 |
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| 摘 要： | 水稻种子16 kDa醇溶蛋白是水稻种子成熟过程中,由16 kDa基因编码,16 kDa醇溶蛋白启动子调控,在胚乳中特异表达的蛋白质.以水稻基因组DNA为模板,通过PCR扩增技术得到16 kDa启动子片段,序列分析结果表明:获得的启动子片段的大小为931 bp,与已报道的该启动子序列相比较,其核苷酸序列同源性为99.9%.该启动子区域含有TATA-box,CAAT-box,GCN4基序,Prolamin-box等胚乳特异表达启动子所必需的正调控元件.利用该启动子构建了植物种子特异表达载体pC16 kDP.
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| 关 键 词： | 水稻 16kDa基因 启动子 醇溶蛋白 |
| 文章编号： | 0253-2395（2007）02-0265-05 |
| 修稿时间： | 2007-02-25 |
Cloning and Constructing of the 16kDa Promoter from Rice |
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| WANG Jing-xue,YANG Cui-pin,LIANG Chao,LIU Li.Cloning and Constructing of the 16kDa Promoter from Rice[J].Journal of Shanxi University (Natural Science Edition),2007,30(2):265-269. |
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| Authors: | WANG Jing-xue YANG Cui-pin LIANG Chao LIU Li |
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| Institution: | 1. School of Life Science and Technology,Shanxi University,Taiyuan 030006 ,China; State Key Laboratory for Biocontrol, Sun Yat-sen University ,Guangzhou 510275 ,China |
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| Abstract: | The 16kDa promoter was amplified from Oryza sativa genome by polymerase chain reaction and cloned into pGEM-T Easy vector.The sequence analysis showed that the obtained promoter fragment 931 nucleotides and shared homology of 99.9 % with the reported 16 kDa promoter.Moreover,the obtained promoter fragment included TATA-box,CAAT-box and the two cis-regulatory elements,GCN4 motif and prolamin-box,which were necessary to mediate endosperm expression of the 16kDa prolamin gene.The plant expression vector pC16 kDP was constructed by the 16kDa promoter. |
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| Keywords: | Oryza sativa 16kDa gene promoter prolamin |
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