低温度系数的高磁导率软磁Mn-Zn铁氧体的研制

利用溶胶一凝胶柠檬酸盐自燃烧法(sol-gel auto-combustion)制备出了低温度系数(αμ=O.2×10-6/℃)的高磁导率(μ=6500)纳米软磁Mn-Zn铁氧体材料。探究了该方法及工艺条件对软磁铁氧体性能的影响,并分析了掺杂少量co2+有利于提高温度稳定性的原因。     

百色盆地百49块百岗组三段高分辨率层序地层学分析

以高分辨率层序地层学理论为指导，通过地表露头、岩芯、测井资料的综合研究，并结合地震剖面资料，分析了百色盆地塘寨油田百49块古近系百岗组三段不同级别的层序界面和湖泛面特征及识别标志，划分出1个长期、3个中期、14个短期基准面旋回层序。在此基础上，详细讨论了各级次基准面旋回层序的垂向序列、岩性岩相组合、结构类型及更加样式等特征，并探讨了高分辨率层序地层学的研究成果在区块油气开发工程中的应用前景。     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system

The human adenovirus type 5 E1A, a tumor- suppressor gene[1], codes for two major related proteins of 243 amino acids (12S) and 289 amino acids (13S) by al-ternative splicing in two exons[2]. Studies have been shown that E1A can regulate expression of many genes and cell cycle[3]. Both in vitro and in vivo experiments indicated that E1A could induce tumor cells differentia-tion, convert tumor cells into an epithelial phenotype, in-hibit tumor cell growth and metastasis and strongly en-ha…     

梯级水库联合理想补偿调节研究

针对梯级水库群防洪调度问题，建立了一个梯级联合优化调度模型，并对此模型提出了一种联合理想补偿调节方式，通过对白山和丰满水库联合调度计算可得到良好的效果。     

东营凹陷沙三段-沙二下亚段T-R层序特征及成因

将东营凹陷古近系沙三段沙二下亚段划分为 3个三级层序 ,即沙三下层序 (SSⅠ )、沙三中层序 (SSⅡ )和沙三上沙二下层序 (SSⅢ )。SSⅠ和SSⅡ层序由湖进体系域和湖退体系域组成T R旋回 ,其特征是代表层序边界的不整合分布局限 ,湖进体系域厚度小 ,主要为细粒沉积物 ,退积式叠加 ;湖退体系域较厚 ,由进积式叠加的砂岩、砾岩和泥岩组成。T R层序的形成主要是由于层序形成早期的构造沉降和气候起主导作用 ,其基底快速沉降 ,湖水供应充分 ,湖平面快速上升。在层序形成晚期 ,沉积物供给和气候起主导作用 ,沉积物供给多 ,快速向前推进 ,湖平面下降。构造沉降和沉积物供给因素对层序控制作用的转换促使其形成层序和体系域的边界。T R层序与三分层序之间的转换是由于层序形成的不同阶段的主控因素不同而引起的     

Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

沥青路面罩面的设计方法

针对国内外罩面或改建设计方法的不足 ,借助于同济大学对新建和罩面改建后的沥青路面使用性能的研究成果 ,将寿命周期费用分析引入罩面设计 ,提出了沥青路面罩面设计方法———基于使用性能和技术经济分析的最佳罩面时机和最佳罩面厚度选择方法 ,并通过实例说明了该方法的应用     

极小展弦比后加载叶栅气动特性三维数值分析和实验

以数值手段分析了极小展弦比下后加载透平叶栅内的定常3D粘性流动及损失，并对该透平叶栅在不同出口Ma和进气角下进行平面叶栅吹风实验．数值方法采用3D可压缩粘性流动压力修正算法求解N-S方程及Baldwing-Lomax代数湍流模型．通过数值计算分析和平面叶栅吹风实验研究表明，后加载技术应用于极小展弦比叶栅中，可以起到控制3D叶栅损失与二次流生成和发展的作用．     

流行装定价策略与价格模型的建立

利用产品生命周期理论对流行装定价策略进行研究,并以流行周期及其所处流行区段为基础构建流行装基准价格模型。通过考察品牌、销售场所、售后服务和市场竞争等因素对流行装价格的影响并探讨各因素权重取值方法,建立流行装市价模型。