| Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells |
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| 作者姓名： | MAOJinshu WANGXianghui CUIYuanlu YAOKanade |
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| 作者单位： | ResearchInstituteofPolymericMaterials,TianjinUniversity,Tianjin300072,China |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，国家自然科学基金，教育部资助项目 |
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| 摘 要： | With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.
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| 关 键 词： | L929细胞 细胞凋亡 细胞周期 透明质酸 壳聚糖 凝胶 生物材料 组织工程学 |
| 收稿时间： | 2002-12-19 |
| 修稿时间： | 2003-04-17 |
Effects of hyaluronic acid-chitosan-gelatin complex on the apoptosis and cell cycle of L929 cells |
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| MAOJinshu WANGXianghui CUIYuanlu YAOKanade.Effects of hyaluronic acid-chitosan-gelatin complex on the apoptosis and cell cycle of L929 cells[J].Chinese Science Bulletin,2003,48(17):1807-1810. |
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| Authors: | Mao Jinshu Wang Xianghui Cui Yuanlu Yao Kangde |
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| Institution: | MAO Jinshu, WANG Xianghui, CUI Yuanlu & YAO Kangde Research Institute of Polymeric Materials, Tianjin University, Tianjin300072, China |
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| Abstract: | With the development in the field of tissue en-gineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on bioma-terials, while cell proliferation and differentiation are stud-ied at the same time. In this paper, hyaluronic acid is incor-porated into the chitosan-gelatin system. Propidium iodide(PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosisanalysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material sur-face, and then cells enter the normal cell cycle quickly. Inaddition, added hyaluronic acid inhibits cell apoptosis trig-gered by the membranes. Therefore, hyaluronic acid im-proves the cell compatibility of chitosan-gelatin system andbenefits the design of biomimetic materials. |
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| Keywords: | hyaluronic acid chitosan gelatin cell compatibility cell cycle apoptosis |
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