Patch clamp technique and biophysical study of membrane channels

Summary The present work describes the patch clamp technique, which first allowed the recording of single channel currents in biological membranes. In particular, it describes procedures for preparation and applications of the four different patch clamp configurations. Briefly, the cell-attached configuration is widely used for investigating channel modulation by transmitters acting via second messengers. The cell-free configurations (inside-out and outside-out), complementary to one another with respect to the orientation of the membrane surface, are particularly indicated for the study of the biophysics (kinetics, conductivity, selectivity, mechanism of permeation and block) of ionic channels. Finally, the whole-cell configuration which, because of the remarkable feature that it allows voltage clamp of very small cells, has given access to a number of physiologically important preparations never studied before.     

碰撞的另一种形式

探讨了碰撞的另一种形式——连接体运动，对连接体运动进行了实验研究，所得出的结论应用于连接体，对两本教科书中提出的连接体问题进行了讨论，结论与教科书中，对连接体运动问题的讨论所得结论截然相反。     

重载钢轨磨耗预测模型及接触斑网格密度研究

基于车辆-轨道耦合动力学及轮轨滚动接触分析,结合材料磨损理论建立了钢轨磨耗预测模型,编制了计算程序,可实现钢轨磨耗具体分布及发展的定量预测分析.鉴于轮轨接触斑离散化网格密度在预测模型中的显著影响作用,从接触力、磨耗分布等方面对这一因素的影响机理进行分析,探讨合理的接触斑网格密度.研究结果表明:网格密度不影响计算结果的正确性,但是稀疏的网格密度得到的蠕滑力及磨耗分布存在较多尖锐形状突变,增大密度可提高精度及磨耗分布平滑性,但会成倍增加计算代价;网格密度20×20时,钢轨磨耗速率变化由剧烈趋于稳定,继续增大密度改善效果已不明显,建议预测模型中接触斑网格密度取20×20,在确保精度的同时尽可能拥有较高计算效率.     

大鼠肾上腺嗜铬细胞原代培养的一种实用方法

以胶原酶Ⅰ、透明质酸酶和ＤＮａｓｅ联用消化肾上腺髓质，再经两次离心收集嗜铬细胞，建立了大鼠肾上腺嗜铬细胞的分离制备及原代培养的实用方法．经反复实验（ｎ＝１２）证明，所获得的细胞形态良好，功能正常．细胞表面光洁、完整，呈球形，直径在８～１２μｍ范围．用细胞贴附式和全细胞方式膜片钳技术在培养２～７ｄ的嗜铬细胞上记录到多种电压敏感性跨膜离子电流，说明培养细胞的膜离子通道完整，适于细胞纯度要求不很高（＜９５％）的单个细胞实验．     

碳化硅晶须增强铝复合材料扩散焊的研究──焊接表面状态对接头性能的影响

By using a Gleeble-1500 thermal and mechanical material testing machine, the authors have welded the Al-Matrix composites material by the diffusion welding method, and studied the effects of the surface treatment conditions of the joint before welding on the joint performance and painted out there are a lot of the different surface treatment conditions. These influences and the influence excessively by high grinding of the surface of joints before welding are helpful to raising the strength of the joint in vacuum diffusion welding. The suitable grinding with suitable welding parameters, the joint strength can reach 90% of that of the base material, and also studied the reason thoroughly by using SEM. XRD. etc.     

一个三维食饵,捕食者,小区域模型

本文探讨一个食饵、捕食者、空小区域之间关系的模型.研究一个三维非线性微分方程组论证它的平衡点的稳定性和周期解的存在性.     

等参双线性元的ZZ超收敛理论分析

分析了ＺＺ超收敛方法，从理论上证明了用等参双线性元求解两阶偏微分方程时，有限元解具有ＺＺ超收敛性。     

Integrating Automatic Patch Snatching System by the Concept of Generalized Mechanisms

An automatic patch snatching system is described which has three machine cells including dividing equipment, tackling equipment and patch removing equipment. The electromechanical integration design of in-piece tackling system based on the concept of generalized mechanisms is introduced. The design of the dividing equipment, the design of micro.feeding machine equipped with micro-level pressure sensor, and the realizing of computer path for patch snatching are investigated.     

竹＋草植被恢复模式斑块特点及其干扰分析

苦竹＋牛鞭草退耕种植5年后，苦竹与牛鞭草呈斑块分布状态．调查分析表明：牛鞭草中的苦竹斑块受人为影响较大，表现出斑块数量、斑块密度和边界密度均大于苦竹林中的牛鞭草斑块，斑块分维数则小于苦竹林中的牛鞭草斑块；而苦竹林中牛鞭草的生长受苦竹林所围成的斑块分维数、边界密度的增大和面积的减小影响逐渐消失；苦竹林中有牛鞭草分布的最小斑块面积在2．4～6．2m2之间，具体需视斑块分维数和边界密度大小而定．本调查分析在一定程度上可为现实的竹斑块、草斑块的经营管理布局提供参考．     

大学生参军报国意识培养机制的构建

大学生参军报国意识培养机制的构建是我国现代化、信息化国防建设、高校征兵工作开展、大学生综合素质提高的必然要求。目前，高校国防教育存在缺乏健全的组织机构、专业的师资队伍、系统的课程体系、创新的教育形式等一系列问题。构建大学生参军报国意识培养机制，可采取健全各级组织机构、加大师资队伍建设、创建合理课程体系、积极创新教育形式等多种途径。