（BN）n及SiB（n-1）Nn（n=2-11）稳定结构的研究

利用遗传算法及Gastreich提出的经验势函数,研究了（BN）n（n=2-11）和SiBn-1Nn（n=2-11）团簇的可能稳定结构,对能量较低的异构体在HF/STO-3G水平进行优化,得到了（BN）n（n=2-11）和SiBn-1Nn（n=2-11）团簇稳定结构单环及线状结构,并进一步分析了单环的结构特征及相对稳定性。     

Finding finer functions for partially characterized proteins by protein-protein interaction networks

Based on high-throughput data, numerous algorithms have been designed to find functions of novel proteins. However, the effectiveness of such algorithms is currently limited by some fundamental factors, including (1) the low a-priori probability of novel proteins participating in a detailed function; (2) the huge false data present in high-throughput datasets; (3) the incomplete data coverage of functional classes; (4) the abundant but heterogeneous negative samples for training the algorithms; and (5) the lack of detailed functional knowledge for training algorithms. Here, for partially characterized proteins, we suggest an approach to finding their finer functions based on protein interaction sub-networks or gene expression patterns, defined in function-specific subspaces. The proposed approach can lessen the above-mentioned problems by properly defining the prediction range and functionally filtering the noisy data, and thus can efficiently find proteins’ novel functions. For thousands of yeast and human proteins partially characterized, it is able to reliably find their finer functions (e.g., the translational functions) with more than 90% precision. The predicted finer functions are highly valuable both for guiding the follow-up wet-lab validation and for providing the necessary data for training algorithms to learn other proteins.     

核磁共振跟踪反应法研究5—O—乙酰基—2.3-双脱氧-D.L-呋喃戊糖基氯的合成

以乙酸四氢呋喃甲酯为原料,通过自由基氯化反应,合成双脱氧核苷类抗艾滋病药物的重要中间体化合物5—0—乙酰基—2.3—双脱氧—D.L—吹喃戊糖基氯.对于此种不稳定的中间体化合物,用~1HNMR跟踪监测反应物的方法,研究了改变反应时间、反应温度和反应物摩尔比对反应产物的影响,获得了最佳的反应条件,使其收率从10％提高到50％左右.     

具有复O－正则拟单调系数的Fourier级数L~1－收敛

本文把文［１］的一个具有复拟单调系数的Ｆｏｕｒｉｅｒ级数Ｌ１－收敛的充要条件推广到具有复Ｏ－正则拟单调系数的Ｆｏｕｒｉｅｒ级数上，所得的结果推广了文［１］和［５］的主要结论．     

钙调神经磷酸酶B亚基高表达条件及产业化研究

重组人CNB高表达大肠杆菌已由本室构建成功,摇瓶发酵液表达量可达100mg·L-1. 通过改进培养基配方及摇瓶表达工艺,使CNB在摇瓶发酵液中表达量达到500mg*L-1以上.在高表达量的基础上,又进行了产业化改进,以蒸馏水添加无机盐取代了原配方中的自来水,并提高了表达量.     

基因工程菌株EscherichiacoliC600（pRLK14）的流加培养

在１Ｌ搅拌发酵罐中对含有重组质粒ｐＲＬＫ１４的大肠杆菌进行了培养。结果表明，采用二段培养法，于３４℃增殖细菌，在对数增殖后期升温到４２℃进行诱导，可以高效表达基因产品半乳糖激酶。诱导期，醋酸浓度增长较快，模拟实验表明，控制醋酸浓度可进一步提高半乳糖缴酶产率。     

中国邮路问题的0-1规划解法

在用“奇偶点图上作业法”求解“中国邮路问题”时,需检查图中的每一个回路.当图中回路较多时,检查不便且易出错.针对此,本文建立了求解“中国邮路问题”的0-1规划模型,并给出了算例。     

船舶二维图形交换研究

船舶图形在不同的CAD系统中交换，共享是现代船舶工业发展的需求，主要论述了如何在2个船用CAD软件（CADDS5和TRIBON M1）之间进行二维图形信息的交换。