排序方式: 共有53条查询结果,搜索用时 109 毫秒
11.
大肠杆菌甜菜碱醛脱氢酶基因的测序及对烟草的转化 总被引:2,自引:0,他引:2
对利用聚合酶链式反应所克隆的大肠杆菌betB基因进行了序列测定,结果证实获得了该基因的克隆pXY01,pXY05以及亚克隆pXY11。将该基因置于CaMV35S启动子调控下,导入根癌农杆菌LBA4404中,叶盘法转化烟草,在含卡那霉素的选择培养基上筛选抗性芽,并进一步诱导小植株的再生。利用PCR技术检测基因整合到烟草基因组中。 相似文献
12.
测定了四种不同土壤的基本理化性质,并分别用其培育心叶烟和三生烟种子,统计两种烟草种子在四种土壤中的萌发率.结果表明:两品种烟草种子萌发率在育烟基质与其他3种土壤间有极显著差异(p〈0.01),而在两烟草品种间差异不显著. 相似文献
13.
14.
s Nicotiana tabaccum ovule extracts induced nuclear reconstitution of demembranated Xenopus leavis sperm in a ceil-free system. Demembranated Xenopus sperm began to swell after 15 rmin of incubation with Nicotiana ovulde extracts. Accompanying the process of incubation,Xenopus sperm decondensed and their shapes changed gradually from long and ellipse to round. The completely decondensed chromatin was surrounded with membrane structure, which was a mixture envelope of a double membrane and a single membrane. Nucleosome assembly was verified by means of micrococcal nuclease digestion to reconstituted nuclei and DNA electrophoresis. Nicotiana ovule extracts supplied one more experimental model and system.The new system could promote powerfully the research on mechanisms of cell division and cell cycle regulation. 相似文献
15.
Nicotiana tabaccum ovule extracts induced nuclear reconstitution of demembranated Xenopus leavis sperm in a cell-free system. Demembranated Xenopus sperm began to swell after 15 min of incubation with Nicotiana ovule extracts. Accompanying the process of incubation, Xenopus sperm decondensed and their shapes changed gradually from long and ellipse to round. The completely decondensed chromatin
was surrounded with membrane structure, which was a mixture envelope of a double membrane and a single membrane. Nucleosome
assembly was verified by means of micrococcal nuclease digestion to reconstituted nuclei and DNA electrophoresis. Nicotiana ovule extracts supplied one more experimental model and system. The new system could promote powerfully the research on mechanisms
of cell division and cell cycle regulation. 相似文献
16.
The camellia isolate of tomato mosaic virus (ToMV-TL) can induce local necrotic lesions on the inoculated leaves in Nicotiana tabacum, whereas the broad bean isolate of tobacco mosaic virus (TMV-B) produces the mosaic symptom on systemic leaves. To examine viral determinant for differential infection phenotype in N. tabacum, the coat protein gene and the 3′ non-coding region of TMV was replaced with that of ToMV, the chimeric virus induced similar local necrotic lesions to that induced by ToMV. The results indicate that the coat protein gene and the 3′ non-coding region of TMV and ToMV influence the virus-induced pathogenesis in N. tabacum. 相似文献
17.
18.
测定了四种不同土壤的基本理化性质,并分别用其培育心叶烟和三生烟种子,统计两种烟草种子在四种土壤中的萌发率.结果表明:两品种烟草种子萌发率在育烟基质与其他3种土壤间有极显著差异(p<0.01),而在两烟草品种间差异不显著. 相似文献
19.
20.
为探明砷胁迫对烟草生理特性的影响,选用本生烟草(Nicotiana benthamiana)为材料,采用盆栽试验,挑选了生长4周后健壮的烟草苗进行砷(As2O3)胁迫处理(50μg·L-1),并于处理后0、24、48、72和96h分别取样并进行相关生理指标(叶绿素含量、丙二醛含量、超氧化物歧化酶、过氧化物酶和过氧化氢酶活性)的测定.结果显示:随着砷处理时间的延长,本生烟草叶片叶绿素含量、超氧化物歧化酶和过氧化物酶活性呈先升后降趋势,过氧化氢酶活性呈下降趋势,丙二醛含量呈上升趋势.试验数据表明:短时间的砷胁迫烟草会出现自我保护现象,但胁迫超出一定时间(24h)后对烟草造成一定的伤害. 相似文献