人甲状旁腺素的基因表达及活性的初步研究

从人甲状旁腺瘤组织中分离到ｍＲＮＡ,逆转成ｃＤＮＡ后,设计带有突变碱基的引物,通过聚合酶链式反应,扩增ｈＰＴＨ基因,使Ｎ端前５氨基酸的碱基富含腺嘌呤核苷（Ａ）．将该基因克隆到表达载体ＰＥＴＩＣ上,转化大肠杆菌ＢＬ２１（ＤＥ３）表达,实验证实ｈＰＴＨ的表达量占菌体蛋白总量的１１％;细胞粗步处理产物经腺苷环化酶的生物鉴定实验证实,重组ｈＰＴＨ具有生物活性,粗产物５００倍稀释后,ｈＰＴＨ的放免活性为２０６．５ｎｇ／Ｌ,为对照的４１５倍     

草鱼生长激素基因在COS7细胞中转染表达的研究

应用RT-PCR技术克隆草鱼生长激素(gcGH)的cDNA,将此cDNA定向插入真核表达载体VR1020中,构建成重组真核表达质粒VgcGH.利用脂质体法使质粒VgcGH转染哺乳动物细胞COS7,对转染后的COS7细胞进行RT-PCR、ELISA和免疫荧光检测,分别在转录和翻译水平证实gcGH基因在COS7细胞中得到了持续和正确的转染表达.     

Alzheimer’s disease: the impact of age-related changes in reproductive hormones

Differences in the prevalence and age of onset of Alzheimer disease (AD) in men and women, and observations that hormone replacement therapy (HRT) may prevent the development of AD, caused many to hypothesize that estrogen deficiency contributes to AD. However, recent trials using estrogen failed to show any benefit in preventing or alleviating the disease. To address this and other inconsistencies in the estrogen hypothesis, we suspect that another hormone of the hypothalamic-pituitary-gonadal axis, luteinizing hormone (LH), as a major factor in AD pathogenesis. Individuals with AD have elevated levels of LH when compared with controls, and both LH and its receptor are present in increased quantities in brain regions susceptible to degeneration in AD. LH is also known to be mitogenic, and could therefore initiate the cell cycle abnormalities known to be present in AD-affected neurons. In cell culture, LH increases amyloidogenic processing of amyloid- protein precursor, and in animal models of AD, pharmacologic suppression of LH and FSH reduces plaque formation. Given the evidence supporting a pathogenic role for LH in AD, a trial of leuprolide acetate, which suppresses LH release, has been initiated in patients.     

Why do hormone receptors arise?

Conclusions In view of the foregoing considerations, it appears that the receptor-hormone relationship is, by origin, essentially a cell-environment (chemical) relationship which influences cell behavior. With the development of multicellularity, the interests of the single (individual) cell became subordinated to those of the cell population (community), and the cell-environment relationship became modified inasmuch as receptor activity became integrated into the functional program of the entire organism. Accordingly, the open program of the individual cell, which involved continuous dynamic changes of the membrane receptors under the influence of the signal molecules, was superseded by a closed program for the given receptor, which gave rise to a chemical memory of the cell. With multicellularity the cellular functions have become integrated into an almost entirely predetermined program in which the quality and operation of the receptors are encoded to maintain the system of regulation, and impart differentiating features to given types of target cells which distinguish them from others, and delimit the response potentials of the species. A limited openness of the pre-programed system exists in the early stage of ontogenesis, and accounts for certain individual variations within the limited potentials of the species.The answer to the question posed in the title of this paper is therefore the following: the hormone receptors arise because the external environment of the individual cell is transformed at the multicellular level to an internal environment, in which the random variety of environmental molecules is replaced by a predetermined set of ligands (signal molecules). Under these conditions the randomlypresented membrane patterns capable of signal reception are transformed to encoded receptor structures which execute a programed function of the closed system, but nevertheless preserve some primordial traits, which can explain many surprising observations in the field of receptor physiology.The Editors wish to thank Professor G. Csaba for having designed and coordinated this review.     

牡丹切花衰老过程中内源激素水平变化的研究

用气相色谱(GC)和高压液相色谱(HPLC)法测定了常温(18± 2℃)和低温冷藏(4± 2℃)条件下牡丹切花衰老过程中内源激素(乙烯、IAA、ABA、CTK)含量的变化,它们对牡丹切花衰老过程的影响以及相互之间的关系进行了初步的分析和讨论。     

基于数字荷尔蒙模型的分布式交通信号控制

在基于荷尔蒙信息的基础上,结合数字荷尔蒙模型,建立了分布式道路交通信号协调控制,并对一个由8个路口组成的交通网进行实验,实验结果表明这种新控制方法的控制效果明显优于定时控制、感应控制和实时控制。     

影响骨形态发育因素研究

骨骼的发育经历了极复杂的过程.众多因素调节了此过程,这些因素包括TGF-β、骨形态发生蛋白、核心结合因子A1、甲状旁腺激素、基质金属蛋白酶、FGF及其受体等.研究了这些因素在骨形成过程中的作用.     

性激素替代治疗对绝经后妇女乳腺的影响

目的探讨性激素替代治疗对绝经后妇女乳腺的影响.方法选择有子宫的、绝经1 a以上的绝经后妇女101人作为研究对象.随机分为两组:A组:46例使用倍美力加安宫黄体酮;B组:55例使用利维爱.连续使用1 a,观察乳房胀痛发生概况、X线钼靶乳腺密度改变情况.并选择同期未用性激素替代治疗的绝经后妇女36例作为对照组.结果组A和组B的乳腺密度增加分别为32.6%和10.9%,与对照组比较组A有显著差异(P<0.05),组B与对照组比较没有显著差异(P>0.05),组A和组B比较有显著差异(P<0.05).结论 HRT治疗中使用利维爱乳腺胀痛发生率较低,对乳腺密度影响轻.     

心脏内分泌研究的进展

心脏除具有“动力”功能外,近年来,科学家证实心脏亦有内分泌功能——分泌心房肽等。心房肽具有很强的利钠、利尿作用;血管扩张作用;参与免疫调节和调节脑屏障作用等。心脏内分泌功能的发现,对心脏功能的研究开辟了崭新领域,对传统的内分泌学说提出了新观念。