高血压大鼠血管平滑肌细胞GRP94和CHOP的表达及替米沙坦的影响

观察替米沙坦对高血压大鼠动脉血管平滑肌细胞内质网应激相关因子葡萄糖调节蛋白94(GRP94)和C/EBP同源蛋白(CHOP)的表达,以及血管中层厚度的变化的影响。将48只成年雄性SD大鼠随机等分为假手术对照组(对照组)、手术模型组和替米沙坦组。对照组分离腹主动脉,但不行狭窄术;模型组行腹主动脉狭窄术(TAC);替米沙坦组行腹主动脉狭窄术并给予每天替米沙坦1.5 mg/kg。喂养6周用颈动脉插管法测定各组大鼠的动脉压(MAP);利用图像分析软件测量血管中层的厚度。用免疫组织化学法和western-blot法检测GRP94和CHOP的表达水平。结果:①手术模型组大鼠的MAP显著高于相应的对照组和替米沙坦组,有统计学意义(P<0.05)。②手术模型组的GRP94表达量高于对照组和替米沙坦组,有统计学意义(P<0.05)。③手术模型组的CHOP蛋白表达量高于对照组和替米沙坦组,有统计学意义(P<0.05)。④手术模型组大鼠血管平滑肌中层厚度厚于对照组和替米沙坦组,有统计学意义(P<0.05)。说明腹主动脉狭窄致高血压可引起血管平滑肌细胞内质网应激反应,导致GRP94表达及CHOP表达的增加。替米沙坦可能通过减轻内质网应激,逆转高血压所致的血管平滑肌细胞的损伤作用,对血管平滑肌细胞有保护作用。     

蜂毒素-SP94杂合基因原核表达载体的构建及蛋白纯化

用可以特异结合到肝癌细胞膜上的小肽SP94,与蜂毒素连接,构建重组表达载体pET32a-蜂毒素-SP94.转入大肠杆菌BL21(DE3),IPTG诱导表达,SDS-PAGE检测表达结果,产物经Ni-NTA Agrosc亲和层析柱纯化后进行Tricine-SDS-PAGE检测.重组蛋白在大肠杆菌中高效表达,约占细胞总蛋白...     

中国国家开发银行贷款项目资金管理信息系统的设计与实现

以软件工程的观点介绍了一个实用信息管理系统的设计过程，给出了需求分析中的数据流程图、数据字典实例，以及系统功能树、实现平台、技术难点、解决方法和运行效率，最后给出了系统的使用评价．     

MOPAC及Gaussian94输出文件管理程序的设计

利用计算化学程序MOPAC、Gaussian94的输出文件自动产生空间构型优化以后的MOP文件和MOL文件，再利用一些免费的共享软件使优化后的化学结构可视化。同时指出并纠正了著名的ChemOffice中的组件Chem3D在图形显示方面的错误，从使用的实用性和方便性方面考虑，开发了一个它们的输入输出文件管理系统，提出了工作效率和计算结果的可靠性。     

GRP1.8融合4CL1基因调控杨树木质素生物合成

将形成层定位启动子GRP1.8与毛白杨的4-香豆酸:辅酶A连接酶基因融合,叶盘法转化毛白杨741,southern杂交检测表明融合基因已经整合到转基因杨树的基因组中.通过实时定量PCR技术测定mRNA的表达量,GC-MS分析4CL底物香豆酸的含量和定量硫酸木质素含量,分析了4CL基因的过量表达对转基因杨树生理生长的影响.实验结果表明,整合了正义4CL1的转基因植株在茎中的酶活性比野生植株增加了30%,木质素含量增加了40%.     

New approaches to therapy of cancers of the stomach,colon and pancreas based on peptide analogs

Cancers of the stomach, colon and exocrine pancreas are major international health problems and result in more than a million deaths worldwide each year. The therapies for these malignancies must be improved. The effects of gastrointestinal (GI) hormonal peptides and endogenous growth factors on these cancers were reviewed. Some GI peptides, including gastrin and gastrin-releasing peptide (GRP) (mammalian bombesin), appear to be involved in the growth of neoplasms of the GI tract. Certain growth factors such as insulin-like growth factor (IGF)-I, IGF-II and epidermal growth factor and their receptors that regulate cell proliferation are also implicated in the development and progression of GI cancers. Experimental investigations on gastric, colorectal and pancreatic cancers with analogs of somatostatin, antagonists of bombesin/GRP, antagonists of growth hormone-releasing hormone as well as cytotoxic peptides that can be targeted to peptide receptors on tumors were summarized. Clinical trials on peptide analogs in patients with gastric, colorectal and pancreatic cancers were reviewed and analyzed. It may be possible to develop new approaches to hormonal therapy of GI malignancies based on various peptide analogs.Received 20 November 2003; accepted 6 January 2004     

变分渐近梁截面分析方法在叶片分析中的应用

针对非均匀的、初始弯扭的各向异性玻璃钢叶片, 由几何非线性的3维弹性理论导出了常规的有限单元横截面分析公式. 基于旋转张量分解的概念, 得到了由1维广义应变与3维翘曲位移表示的3维应变场. 根据1 维应变, 用变分渐近方法建立翘曲位移, 然后可以得到具有任意几何形状和材料特性的玻璃钢叶片的横截面刚度. 作为应用实例, 计算了1 5 MW变速恒频风力机玻璃叶片截面剪切中心位置分布和截面刚度矩阵.     

Detection of HLA-E and -G DNA alleles for population and disease studies

HLA-E and -G genes show a restricted polymorphism encoding for molecules whose variability is limited at the peptide binding site. Fourteen alleles that give rise to only three productive proteins for HLA-G (*0101, *0103 and *0104) and five alleles with three different proteins for HLA-E (*0101, *0102 and *0103) have been described. Expression of these molecules is low and found in many tissues for HLA-E; HLA-G protein is expressed in extravillous trophoblast cells and thymic epithelium. Molecular studies have shown how HLA-G and HLA-E bind to natural killer (NK) cells immunoglobulin and lectin-type inhibitory receptors. HLA-E may act as a sentinel of the cell; if classical class I and HLA-G are being expressed, HLA-E molecules may reach the cell surface and inhibit the lysis by NK cells. Most findings are consistent with the hypothesis that HLA-E and -G proteins may be tolerogenic molecules at either the T-cell receptor (TcR) (inflammation, graft rejection) or NK level, switching off cells which usually attack foreign (including foetus) or self (autoimmune) antigens. A low HLA-E and -G polymorphism is observed in humans, and their allele frequencies are mostly homogeneous in the populations tested so far. Many studies to detect these alleles are now being performed in isolated populations and also in pregnancy-associated pathologies. In the present paper, standard and detailed techniques to detect HLA-E and -G DNA polymorphism are reported and discussed. Received 14 July 1999; received after revision 25 August 1999; accepted 25 August 1999     

匿名技术的抗攻击性研究

研究了匿名技术的抗攻击性问题，定义了基于重路由的匿名通信系统模型，对匿名技术可能受到的攻击进行了分类和定义，分为消息码攻击、消息长度攻击、重放攻击、合伙攻击、泛洪攻击、消息量攻击、时间攻击、侧面攻击等8种，并对现有的主要匿名技术在抵抗攻击性方面进行了研究，为匿名技术的研究和设计新的匿名系统提供了依据．     

IM-94增强小鼠免疫功能的实验研究

研究了ＩＭ９４ 对免疫低下小鼠免疫功能的影响．结果显示：ＩＭ９４ 能逆转环磷酰胺所致的免疫低下反应,并进一步提高小鼠的免疫活性,使其巨噬细胞吞噬能力、ＮＫ 细胞自然杀伤活性、淋巴细胞增殖水平、半数溶血值和抗体生成细胞水平均有明显提高,说明ＩＭ９４ 对实验小鼠有正向免疫调节作用．