Unfolding of C2A Domain of Synaptotagmin I in the Presence of Guanidine Hydrochloride

The C2 domain originally referred to the second of four constant structural motifs in protein kinase C (PKC). Now this domain represents a large structural family sharing a homologous dimensional structure in many proteins that play important roles in many organisms. The C2A domain is one of the two C2 domains of synaptotagmin I involved in the Ca^2 regulation of exocytosis. This domain is mostly composed of β-sheet except for a small fraction of α-helix, and therefore provides an ideal model for a protein folding study. In this report, the unfolding equilibrium of the C2A domain in guanidine hydrochloride (GdnHCI) containing solutions has been studied using ultraviolet (UV) difference spectrum, fluorescence spectrum, size exclusion chromatography (SEC), and circular dichroism (CD) spectrum. The results suggest that unfolding of the C2A domain occurs as a two-state process during GdnHCI titration. By examining the changes of both tertiary structure and secondary structure, no intermediates could be detected during this unfolding study. However, it has been found that the native state of the C2A domain has a large hydrophobic surface. This result suggests that as a fragment of a protein, the C2A domain itself may exist in a state with large hydrophobic surface. This hydrophobic surface may be the molecular basis for interaction between domains in the whole protein.Furthermore, the hydrophobic behavior may play a role during the oligomerization of svnaptotagmin.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system on the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the sensitivity of chemotherapy and radiotherapy. E1A have the ability to integrate into the host genome, resulting in long-time expression that induces Rb gene inactivation and animal cells immortalization. This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy. Thus, we firstly constructed E1A eucaryotic expression vector (pPIC9/E1A), transformated the pichia pastoris yeast cells (GS115) and screened the high-expressing recombinant strains. The positive yeast strains were cultured in the shake flask, and induced for 3 d. The crude E1A protein was purified using two steps of column chromatography on HiTrap Q and HiTrap SP. The purified E1A protein was identified by SDS-PAGE and Western blot. E1A protein was mostly located at cellular nuclear when Chariot delivered E1A protein into cells. The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase, and significantly inhibited the growth of LN686 tumor cells. The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

基于CPLD的高速音频数据采集系统的设计及实现

介绍了在地下漏水探测仪中用CPLD实现高速音频数据采集控制及与单片机的接口逻辑设计，其中，总线接口，A／D采样控制，双端口RAM缓存，控制译码，时序逻辑电路均由CPLD实现，整个系统控制灵活，结构紧凑，体积小，功耗低，抗干扰能力强，适合于室外作业．     

关于二阶欧拉方程的求解

欧拉方程一般都是用“变量代换”法求解的，但其过程一般都比较繁琐，直接用初等积分法给出了求二阶欧拉方程的通解的一般公式，此方法简单且适用范围广。     

关于Au-内射模的某些研究

讨论Aμ—内射模的性质，引入有限Aμ—内射模的概念，并利用它给出环的刻划。     

低碳钢A-TIG焊活性剂的焊接性研究

利用自行研制的低碳钢A TIG焊活性剂进行了各种焊接工艺试验 .试验结果表明 ,使用本活性剂可使焊缝熔深比常规的TIG焊增加 3倍 ,对 12mm以下的低碳钢板对接无须开坡口 ,可一次焊接完成并能单面焊双面成型 .焊缝表面成型良好 ,接头微观组织、焊缝的化学成分和接头力学性能均不受影响 .焊接电流、弧长、焊接速度、涂层厚度和气体种类等均对焊接熔深的增加产生影响     

红霉素肟主杂质的分离和鉴定

深入研究了红霉素的肟化反应.分离和鉴定了在合成红霉素肟的过程中产生的一种主要杂质,通过IR,UV,MS,1H NMR和13C NMR确证它和红霉素在酸性水解时生成的红霉素A 8,9-脱水-6,9-半缩酮为同一化合物.这一结果说明,在酸的存在下,红霉素的肟化反应总是伴随有它的酸性降解反应.因此,在合成红霉素肟时,严格控制作为催化剂的酸的用量是极为重要的.     

初孵扬子鳄中脑视叶组织学结构观察

用光镜对扬子鳄中脑视叶的组织学结构进行了研究.从背侧到腹侧,扬子鳄中脑视叶可分顶盖和被盖两部分,二者之间无明显界线,细胞排布有所区别.从外侧到内侧,顶盖可分为带状层、外灰质层、浅白质层、中灰质层、中白质层、深灰质层、深白质层和中央灰质. 被盖前部分层与顶盖相同,中后部分层不明显.神经细胞主要在灰质内,白质中零星分布一些小细胞.视叶室的形状由前向后不同.同时对扬子鳄中脑视叶同其他脊椎动物的进行了比较讨论.     

电子干扰下GPS接收机C/A码捕获性能的研究

研究电子干扰对卫星定位系统(GPS)接收机C/A码捕获性能的影响.分析了C/A码平均捕获时间和捕获时间方差,并且进行了基于连续波、宽带及部分频带3种干扰模式的模拟分析.分析结果表明,若针对GPS接收机的干扰功率超过一定强度后,平均捕获时间和捕获时间方差指标将急剧恶化.在相同的GPS接收机中频输出信干比下,连续波干扰较宽带干扰效果更加明显.     

近液相线半连续铸造非枝晶A-356合金半固态触变成形

采用电子显微镜、扫描电镜及电子拉伸机,研究了液相线半连续铸造法制备的A356合金半固态浆料的组织,重熔加热的合金组织,经触变成形及固溶时效处理后的组织与性能·结果表明,触变成形并经热处理后的A356合金,析出相为Al3Si,AlSi等化合物,其性能得到显著提高·经触变成形得到的汽车用轴瓦盖毛坯,其σb值达到2812MPa,δ5达到138%,此结果为用近液相线半连续铸造法制备非枝晶A356合金触变成形的深入研究奠定了基础·