接骨草蔗糖酶同工酶的分离纯化及其部分性质研究

从接骨草幼叶中提取的蔗糖酶粗酶液经硫酸铵分级沉淀、DEAE-Sepharose离子交换层析、Sephacryl S-200凝胶过滤层析纯化，得到3种同工酶．对3种同工酶进行SDS-PAGE和IEF电泳均显示一条蛋白带，其亚基分子量分别为45．8KD，44．3KD，43．2KD；等电点分别为pH4．0，pH3．8，pH3．75．凝胶过滤测得3种同工酶全酶分子量分别为91．2KD，89．1KD，87．4KD，表明3种同工酶均由2个相同亚基组成，其表观Km分别为30mmol／L，57.1mmol／L，65mmol／L;Vmax分别为98μg／min，48μg／min，35μg／min．同工酶Ⅰ和同工酶Ⅲ在pH4．4～5．0时有最大活性，同工酶Ⅱ则在pH3．9～4．2时有最大活性．3种同工酶均在pH3-6范围内比较稳定．同工酶Ⅰ和同工酶Ⅱ在48～52℃有最大活性，同工酶Ⅲ在50～55℃有最大活性．3种同工酶均在50℃以下有较好的稳定性．     

Construction of a genetic map with SRAP markers and localization of the gene responsible for the first-flower-node trait in cucumber (Cucumis sativus L.)

With an F2 population from the cross of two cucumber inbred lines, S06 and S52, sequence-related amplified polymorphism (SRAP) was used to construct a genetic linkage map in cucumber (Cucumis sativus L.). Sixty-four SRAP primer combinations generated 108 polymorphic bands in the F2 population analysis. The average of polymorphic bands produced by one primer pair was 1.5, and the maximum was 5. Using Mapmaker 3.0, a linkage map was constructed, which consisted of 77 SRAP markers distributed in nine linkage groups (LOD≥3.0) and spanned 1114.2 cM with an average interval of 14.5 cM between markers. The gene for the first-flower-node trait, termed ffn, was mapped to linkage group Ⅸ, flanked by DC1EM5 and ME7EM2A at 10.3 cM and 12.1 cM distance, respectively.     

苦豆子、铁棒锤生物碱对菜青虫的毒力测定

苦豆子、铁棒锤生物碱对菜青虫毒力测定的结果表明，苦豆子、铁棒锤生物碱对菜青虫均有较好的胃毒作用，用致死中量表示苦豆子生物碱LD50＝30．07μg/g，铁棒锤生物碱LD50＝38．83μg/g．室内触杀作用试验和田间防效试验表明，苦豆子生物碱有效剂量为2000μg/mL、600μg/mL和铁棒锤生物碱有效剂量为2000μg／mL时，对菜青虫有很好的防治效果，在施药后72h，校正防治效果可速80％-90％，且仍呈逐渐增大趋势(活虫数逐渐减少趋势)．     

中药菟丝子花粉形态的扫描电镜研究

利用扫描电子显微镜对山东菟丝子属(Cuscuta L.)3种植物的花粉形态进行了观察研究。结果显示该属花粉粒为长球形，具3～4沟。表明该属花粉粒大小、孔沟数量和外壁雕纹可以作为种间分类的依据。     

普通小麦面筋物理品质性状的双列分析

本研究对沉淀值、比沉淀值两个面筋物理品质性状利用Hayman方法进行的分析表明，两性状低值表现部分显性，高值与隐性效应相联系．加性方差和显性方差分量均达显著，基因的加性效应比显性效应更重要．     

野生大豆DNA导入小麦及RAPD分子验证

利用花粉管通道法将野生大豆总DNA导入小麦，以期获得变异系小麦．对小麦球蛋白的SDS—PAGE，印迹表明，冬小麦T2l6#产生了新的蛋白亚基，其分子量为78kD，而有的变异品系一些蛋白亚基消失了；凯氏定氮法和氨基酸分析表明小麦后代T216#的总蛋白含量增加，其氨基酸组成，尤其是赖氨酸含量明显提高．RAPD分析结果表明，新品系基因组出现多态性，并具有供体特异性DNA带，这些变异现象表明该小麦品系为转基因后代．本实验为选育高蛋白、优质的新小麦品种提供了途径．     

水稻精细胞基因RSSG58启动子的克隆分析及表达载体构建

根据分布的水稻基因组测序的比较和水稻精细胞优势表达的RSSG58基因cDNA序列，以水稻品种“桂朝2号”黄化苗组DNA为模板，用PCR方法克隆出RSSG58在起始密码子以前的上游调控序列Pr58，经过Pr58启动子进行的鉴定和分析表明，具备大多数高等植物启动子的保守元件，预计它的RSSG58基因在特异表达方面具有一定的作用。为了鉴定RSSG58基因的基本启动子元件，将RSSG58基因5′侧翼序列做缺失片段分析，由PCR从Pr58中得以3个不同大小两端带有HindⅢ，BamHⅠ酶切位点的片段Pr58Ⅰ，Pr58Ⅱ和Pr58Ⅲ，定向插入载体pMGFP4(pBI221改建，报告基因为GFP）中，取代原有的CaMV35S启动子，构建了由驱动报造基因GFP的植物表达载体pRGFPⅠ，pRGFPⅡ和pRGFPⅢ，用于农杆菌介导法水稻遗传转化。其目的是为进一步在模式植物中研究其表达功能奠定基础。     

非线性拟双曲方程的特征变网格有限元方法和分析

对神经传播过程中的一类非线性拟双曲方程的初边值问题的三维情形应用常规变换，提出了特征变网格有限元格式，最后通过细致的分析和估计得到了最佳阶的L^2模误差估计结果。     

红豆蔻挥发油化学成分的GC/MS法分析

用水蒸气蒸馏法从红豆蔻中提取挥发油．采用不同类型的毛细管柱进行分析，找出最佳分析条件，用归一化法测定其百分含量，并用气相色谱-质谱法对化学成分进行鉴定．鉴定得到48个成分，占挥发油总成分的84％以上．     

N-terminal of L protein of vesicular stomatitis virus contains a new signal sequence

The L protein (241kD) of vesicular stomatitis virus (VSV) is the most important snbnnit of the replication complex. The existence of specific localization signal in the L protein was investigated by making recombinant constructs expressing truncated mutants of the L protein fused to green fluorescent protein (GFP) in transient transfection assays. The chimeric genes encoding varied N-terminal of L and GFP gene were put under the control of T7 promoter or CMV promoter. The fusion proteins were transiently expressed in BHK-21, COS-7, CHO or Hep G2 cells. When more than 120 residues were deleted or only 96 residues were kept on the N-terminal, the fusion proteins were shown to be distributed throughout the cells, cytoplasm and nucleus under the confocal microscope. However, other chimeric proteins with 120 or more amino acids were dotted and distributed in the perinuclear regions. And the fusion protein with 96—120 aa has the similar distribution. A thirteen-residue peptide QGYSFLHEVDKEA (108—120) was identified as localization signal, whose function would be absolutely distributed with the deficiency of D or V. Our results show that there is an independent localizing signal in N-terminal domain of L protein of VSV and this functional signal is conserved in different cell lines.