不同方法处理的角膜脱细胞基质组织学生物特性的比较

目的:比较不同的角膜脱细胞基质方法,旨在选择最适合的脱细胞基质方法,制备成为组织工程角膜支架材料和角膜替代物的脱细胞基质.方法:分别用胰酶-曲拉通法、SDS-戊二醛法、胰酶-冷冻法等方法对角膜进行脱细胞处理,并对脱细胞彻底的基质进行力学特性、透光率的分析.结果:用胰酶-冷冻法获得的兔角膜全层和分层基质脱细胞最彻底,基质的结构未见明显破坏,脱细胞基质在生物力学特性上与正常角膜接近,透光率低于新鲜角膜.结论:用胰酶-冷冻法获得的脱细胞基质与自然角膜接近,可作为组织工程角膜支架材料和角膜替代物.     

Ecdysteroids in the larvae of Opogona sacchari （Bojer）, a invasive alien pest

In order to understand the composition and quantitative variation of ecdysteriods in the larvae of Opogona sacchari (Bojer), a invasive alien pest, we analyzed the larval ecdysteroid composition and titers in this pest. The main component of ecdysteriods in the larvae of O. sacchari is 20-hydroxyecdysone, and also there is a little 26-hydroxyecdysone. The titer of ecdysteriods in the larvae from the 1st instar to the 7th instar was gotten higher gradually compared on ng ecdyster-oid/larva, but no regularity could be found about the titer if compared on ng ecdysteroid/g avoirdupois. There was only one peak of ecdysteroids (0.5475 ng/larva) showed at day 2 during the developmental time of the 6th instar larvae. However, there were two peaks appearing during the developmental time of the 7th instar larvae , one peak (0.29415 ng/larva) at day 3, another (0.214 ng/larva) at day 5.     

Biomineralization of phototrophic microbes in silica-enriched hot springs in South China

Microbial mats in two hot springs in South China were sampled for the research of mineralization of microbes and its mechanism by the methods of geology and modern biology. The results show that hot spring microbes have the key capability for enrichment of Si, Al, Fe, Ca and other elements, and the microbes are also crucial for the formation of SiO2, CaCO3, clay and so on. The extracellular polymeric substances (EPS) play important roles in the process of mineralization of hot spring microbes, which mainly takes place in the layer of EPS outside cell wall or sheath of cyanobacteria. The sheath outside cell wall, which keeps the normal metabolism of cyanobacteria during the process of mineralization on its surface, is also considerable for the biomineralization of cyanobacteria. According to structure and mineralization characteristics of two microbial mats, the process of mineralization can be divided into three stages, namely, early surface mineralization, middle degradation mineralization, and late des- quamation of mineral. The above conclusions are significant for comprehension of the process of mineralization, the process of deposition and the preservation of microfossil in modern and ancient extreme environments.     

Study on peptide-peptide interaction using high-performance affinity chromatography and quartz crystal microbalance biosensor

The specific interaction between sense and antisense peptides was studied by high-performance affinity chromatography （HPAC） and quartz crystal microbalance （QCM） biosensor. Fragment 1-14 of human interferon-β （hlFN-βwas chosen as sense peptide and its three antisense peptides （AS-IFN 1, AS-IFN 2, and AS-IFN 3） were designed according to the degeneracy of genetic codes. The affinity column was prepared with sense peptide as ligand and the affinity chromatographic behavior was evaluated. Glu-substituted antisense peptide （AS-IFN 3） showed the strongest binding to immobilized sense peptide at pH 7.5. A quartz crystal microbalance-flow injection analysis （QCM-FIA） system was introduced to investigate the recognition process in real-time. The equilibrium dissociation constants between sense peptide and AS-IFN 1, AS-IFN 2 and AS-IFN 3 measured 2.08×10^-4, 1.31×10^-4 and 2.22×10^-5 mol/L, respectively. The mechanism study indicated that the specific recognition between sense peptide and AS-IFN 3 was due to sequence-dependent and multi-modal affinity interaction.     

鄂东大别山生物多样性研究

对鄂东大别山区的生物多样性进行了研究.目前已知该区有维管植物195科663属1 461种,占湖北省总种数的24.27%和全国总种数的5.25%,属国家特有、珍稀濒危或重点保护的植物有34种,有苔藓植物30科64属143种.已知该区有陆生脊椎动物26目65科208种,占湖北省总种数的31.04%和全国总种数的8.09%,其中有27种国家重点保护野生动物和61种湖北省级重点保护动物.该区自然植被类型有10个植被型,52个群系.该区不仅动植物区系成分多样性复杂,而且经济类型多样.     

低质量浓度废水厌氧生物处理的动力学模拟及工程实践

介绍了厌氧生物反应原理,进行了厌氧废水处理的动力学分析,建立了低质量浓度废水厌氧处理模型并进行了工程实践。     

钛酸钾在人工生理环境中的行为

为了研究不同钛酸钾的生物医学特性,利用模拟体液模拟生理环境,进行了初步分析。光学显微镜和扫描电镜观察表明,层片状低钛酸钾在去离子水中向高钛酸钾转变,而在模拟体液中则不能。选区电子衍射分析表明,在模拟体液中低钛酸钾吸收了Ca2 ,而六钛酸钾则诱导了富含Ca/P颗粒的沉积。说明六钛酸钾具有更好的生物相容性和生物活性。     

转笼生物反应器流场的滑移网格与动网格计算比较

转笼生物反应器是利用动态流化床的理念研制的一种新型反应器,其内部流场直接影响反应器的污水处理效果,采用计算流体力学方法(CFD)模拟其内部流场,对于优化转笼结构,提高污水处理效率有着重要作用.通过采用滑移网格与动网格两种方法对转笼内部流场进行计算比较,分析表明:滑移网格在模型处理、复杂运动边界模拟和计算结果连续性方面不及动网格模型;滑移网格在计算耗费方面优于动网格模型.     

黄河三角洲生物多样性特点及保护对策

根据黄河三角洲地区所具有的植物区系成分简单,植物种类少,以及稳定性差,植物区系年轻等特点,提出了具体的保护对策。     

工业废水生物毒性监测与综合评价

为了研究水生生物毒性试验方法在工业废水监测和管理上的应用，通过对辽宁省重点工业源废水进行鱼类、发光菌、蚕豆根尖的生物毒性试验研究。试验结果表明，工业废水和污染物的排放量、生物毒性监测指标及综合生物毒性指数间存在着密切的关系。应该对这些重点企业废水采取进一步的治理措施，否则会影响地表水环境质量及水生生物的生存。