低频电脉冲技术应用于产后催乳与子宫复旧的临床研究

目的:研究低频电技术在产后催乳与子宫复旧方面的应用。方法:对30例产后3天无乳汁或泌乳不足的研究组病例应用SOKO900型多处方治疗仪,对照组不应用仪器观察泌乳始动时间、泌乳量、子宫底下降速度等。结论:采用低频电脉冲技术原理的SOKO900型多处方治疗仪适用于产后催乳治疗、促进子宫复旧,有显著疗效。     

地形图法在体表子宫肌电信号监测中的应用

将地形图方法应用于体表子宫电信号监测,为此领域提供了新的分析和显示处理方式．此方法是在借鉴脑电分析中地形图方法的基础上,通过建立模型加以实现的．临床测量结果表明,此方法与传统方法相比,具有清晰、直观的优点,特别是能够清晰地显现宫缩收缩的协调性,为临床的产程监护和医学研究提供了一种有力的手段．     

剖宫产子宫瘢痕妊娠的介入治疗评价

目的：对剖宫产子宫瘢痕妊娠的介入治疗-MTX子宫动脉灌注化疗＋子宫动脉栓塞术的效果进行评价。方法：将我院2005年1月至2008年1月共8例剖宫产子宫瘢痕妊娠采用以介入治疗为主要方法的结果进行分析。结果：MTX子宫动脉灌注化疗＋子宫动脉栓塞术,能快速止血,使胚胎死亡和血β-HCG下降,效果良好。但血β-HCG下降速度不一。血β-HCG〈15000IU／ml者,每48小时下降30～50％;而血β-HCG≥15000IU／ml者下降缓慢。加行B超引导下胚胎清除术,能使血β-HCG迅速下降,明显缩短住院时间。结论：MTX子宫动脉灌注化疗＋明胶海绵栓塞术,是一种有诸多优点,极其有效的治疗方法。如与B超引导下胚胎清除术结合使用,应是最佳治疗手段,值得有条件的医院大力推广。     

两组浓度的蛋白酶体抑制剂MG132诱导人宫颈癌细胞Hela凋亡

为了探讨不同浓度蛋白酶体抑制剂MG132在诱导人宫颈癌Hela细胞凋亡过程中作用,分别以两组浓度的蛋白酶体抑制剂MG132处理人宫颈癌细胞Hela,通过MTT法检测Hela细胞活力,应用AnnexinⅤ和PI双染的细胞流式法检测Hela细胞凋亡率,应用Western blotting和酶标仪法检测Hela细胞内Caspase-3活性变化的情况.结果表明:低浓度MG132诱导Hela细胞凋亡不明显,高浓度MG132诱导Hela细胞凋亡明显;不同浓度蛋白酶体抑制剂MG132诱导人宫颈癌Hela细胞凋亡的程度存在明显差异.     

基因工程菌株产生的含人类绒毛膜促性腺激素抗原的研究 Ⅲ.β—Galactosidase—hCG 免疫和生物活性的初步探讨

基因工程菌株产生的含人类绒毛膜促性腺激素(hCG)β亚单位 C 末端36肽的β-半乳糖苷酶杂交蛋白(简称:β-Galactosidasc-hCG),主动免疫昆明种小白鼠,能诱发特异抗 hCG 抗体的产生。其抗体滴度的高低与免疫原用量有关。通过对小鼠子宫增重抑制试验,表明其抗血清能中和 hCG 的生物活性。     

Effects of PGE2 and PGF2α on the simulation by noradrenaline and oxytocin of human cervical muscle activity at term

Summary Cervical specimens were obtained by needle biopsy in connection with caesarean section at term pregnancy. The preparations were superfused in an organ chamber and contractions were registered isometrically. Prostaglandin (PG) E₂ and F₂ inhibited spontaneous contractions. The stimulatory action of noradrenaline was not influenced by PGF₂ but was reduced by PGE₂ whereas both PGs abolished the excitatory effect of oxytocin.     

Embryo implantation: A time for recalling and forwarding

The success of embryo implantation is a critical step towards further embryo development and pregnancy outcome. The observations and investigations on embryo implantation have been over a century. A huge body of knowledge has been accumulated in anatomy, histology, ultrastructure and hormonal regulation; as well as recently in depth information about molecular signaling pathways got from studies of genomic wide gene screening and specific gene deletion. The knowledge from basic research has also substantially helped to initiate and improve the Artificial Reproductive Technology （ART） in clinical applications. Now we＇ve known that the normal embryo implantation involves the embryo＇s development into an implantation-competent blastocyst and the synchronized transformation of uteri into a receptive stage. The interdependent relationship between the blastocyst and uterus involves complicated hormonal regulation and local paracrine, juxtacrine interactions. In this paper, we review some important historical findings regarding uterine receptivity and blastocyst activation, as well as some less discussed topics such as embryo spacing, embryo orientation. Further understandings on detailed mechanisms during the process of embryo implantation will help cure women infertility as well as develop new generation of non-steroids contraceptives.     

邻苯二甲酸丁基苄酯与邻苯二甲酸二丁酯的雌激素生物活性比较

通过采用体内小鼠子宫增重法检测环境雌激素邻苯二甲酸丁基苄酯(BBP)和邻苯二甲酸二丁酯(DBP)的雌激素活性.结果表明,BBP和DBP都具有较强的雌激素效应,且BBP的雌激素效应大于DBP.     

Reversal Efficacy of SDZ-PSC833 and Verapamil on Drug-Resistant Human Cervical Carcinoma Cells Line

This article discusses the characteristics of the drugresistant human cervical carcinoma cell line HeLa/MMC and investigates reversal effect of SDZ-PSC833（PSC833） and Verapamil （Ver） upon HeLa/MMC. Relative content of cell DNA detection, H3-TdR incorporation test and drug-resistance have been experimented, meanwhile a tumor transplant model in the nude mice for Hela/MMC has been established. Then the volume of cancer, tumor weight, curve line of growing up in tumor -bearing nude mouse and its morphological variation of carcinoma organism are observed to evaluate the reversal effect of PSC833 and VER. It has been found that： （1） Compared with HeLa cells,subline HeLaJ MMC Cells＇ drug-resistant capability against Mitomycin（MMC） or cisplatin（DDP） is as 5.02 folds or 2 folds as that of the former, respectively. The Hela/MMC cell line has characteristics of multi- drug resistance and stronger multiplication; （2） PSC833 and VER can reverse resistance of HeLa/MMC to Mitomycin in vivo.PSC833 will be a better candidate for reversing multidrug resistant than verapamil in clinic application.     

产后奶牛子宫机能复旧治疗方案的研究

为了探讨产后奶牛子宫机能恢复的有效治疗方案,本试验随机选择生殖正常的荷斯坦奶牛,在产后特定时间内注射缩宫素、氯前列烯醇、苯甲酸雌二醇、促黄体释放激素A3,并结合中药制剂和土霉素,直肠检查产后子宫形态,观察产后子宫恶露排放情况以及发情时间,统计受胎率,分析产后奶牛子宫机能的恢复状况。结果显示:试验组(A组)产后奶牛在30和45 d内发情率分别为75%、85%,60 d内的受胎率为55%,极显著高于对照组(B组)。A组中恶露排尽的时间,子宫孕角以及子宫颈恢复的时间要比B组提前,差异极显著(P0.01);由此得出:以缩宫素、氯前列腺素、苯甲酸雌二醇、促黄体释放激素A3结合中药制剂和土霉素的治疗方案,可有效的加快奶牛产后恶露的排放和子宫机能的恢复,而且可以有效的预防和治疗子宫内膜炎、卵巢囊肿等产后疾病,起到了提高受胎率,缩短产犊间隔的作用。