网络联盟企业中制造单元生产信息管理

敏捷制造将成为制造业在21世纪采用的主要模式,网络联盟是实现敏捷制造的基本组织方式。本文对网络联盟企业的概念、总体构思及主要支撑技术进行了简要介绍,指出信息管理是网络联盟企业中一项非常重要的工作;而制造单元是网络联盟企业的基本组织管理单位和制造过程的执行单位,是网络联盟企业物流与信息流的交汇点,所以,制造单元的生产信息管理是整个网络联盟企业信息管理工作的重要内容。本文还就制造单元生产信息管理的内容及技术进行了论述,指出单元生产调度是制造单元生产信息管理的重要内容,对网络联盟企业中制造单元的生产调度和制造单元的运行进行了重点介绍。     

3Rs原则的应用实例——用鸡胚肝细胞替代鸡肾细胞培养禽腺病毒

在动物实验中利用减少 (reduction)、替代 (replacement)和优化 (refinement)的 3Rs原则 ,是执行实验动物福利的重要体现。利用SPF鸡胚肝细胞培养禽腺病毒 ,用于制备诊断抗原和病毒研究 ,取代用SPF鸡肾细胞培养禽腺病毒 ,通过观察细胞病变和测定抗原滴度分析比较 ,前者完全可以替代后者制备禽腺病毒诊断抗原 ,是利用 3Rs原则的具体实践。尽可能利用可替代实验动物的替代物 ,通过细胞组织培养的方法取代活体动物进行实验 ,将动物的痛苦减至最低     

成纤维细胞和ES细胞在小鼠和兔去核卵母细胞内发育

以昆明白小鼠成纤维细胞和胚胎干（ES）细胞作为供核细胞,以昆明白小鼠和日本大耳白兔的MⅡ期去核卵母细胞作为受体,采用核移植方法,构楚了克隆胚胎．在同种克隆中,以ES细胞为供核细胞的克隆胚胎卵裂率明显低于以成纤维细胞为供核细胞的克隆胚胎卵裂率（24．4％相对于56．9％,P〈0．05）,1．8％的ES细胞克隆胚胎发育到囊胚阶段,而成纤维细胞克隆胚胎没能发育到囊胚阶段;在异种克隆中,以ES细胞为供核细胞的克隆胚胎卵裂率（89．6％）和囊胚发育率（18．8％）明显高于以成纤维细胞为供核细胞的克隆胚胎卵裂率（54．2％）和囊胚发育率（4．2％）．     

基于信道多普勒特征的外辐射源雷达杂波抑制方法

利用数字广播信号的正交频分复用(orthogonal frequency division multiplex, OFDM)调制特性,提出一种基于信道多普勒特征的外辐射源雷达杂波抑制方法。首先,在子载波域获取每个OFDM符号的频域信道响应值,然后将该信道响应值变换到时域,并在时域利用信道多普勒特征构造杂波子空间项,用于抑制零频和非零频杂波。所提方法杂波子空间构造与发射信号无关,可离线生成,简化了信号处理流程,降低了计算复杂度。仿真和实测数据均验证了所提方法的有效性。     

无环境信息下多尺度网格细胞群空间表征模型

针对无环境信息条件下网格细胞空间表征问题, 提出一种多尺度网格细胞群的空间表征模型。通过减少吸引子网络模型网格细胞的偏好朝向, 使网格细胞具有特定的感应方向, 仅对特定方向上的速度产生响应。基于改进的吸引子网络模型, 构建多尺度网格细胞群, 并对空间进行表征。仿真结果表明, 改进后的网格细胞模型只响应相应方向的速度, 准确整合速度信息, 表征部分未探索区域, 且适用于无环境信息条件下一维、二维空间。在100 m×100 m的区域, 表征结果的误差不超过0.2 m, 证明了该模型能够扩大网格细胞的表征范围, 提高表征精度与表征能力。     

A new DFM flow for sub-100nm standard cells

DFM （Design-For-Manufacturability） method, which aims to improve manufacturability of ICs through specific design considerations, is becoming important nowadays. In particular, standard cells now should be designed by DFM method. This paper reports a new DFM flow for sub-100 nm standard cell design with a group of technologies for process modeling, manufacturability simulation and trial RETs. Based on this flow, a set of DFM-friendly 90nm standard cells were designed.     

Increased production of recombinant prourokinase with porous microcarrier cell culture by periodic pressure oscillation in a stirred tank reactor

0 IntroductionIn microbiological fermentation and plant cell cul-ture ,there are manyexamples of applying“periodic oper-ation”to enhance productivity[1-3],although the mecha-nismof this effect remains obscure .In animal cell cul-ture ,applying intermittent hydrostatic pressure could in-crease cell proliferation[4-6]orincrease enzyme activity[7].Process parameters such as temperature , pressure , pH,light intensity and nutrients concentration may be chosentointroduce physical stimuli .Cytopo…     

Temperature modeling and control of Direct Methanol Fuel Cell based on adaptive neural fuzzy technology

Aiming at on-line controlling of Direct Methanol Fuel Cell （DMFC） stack, an adaptive neural fuzzy inference technology is adopted in the modeling and control of DMFC temperature system. In the modeling process, an Adaptive Neural Fuzzy Inference System （ANFIS） identification model of DMFC stack temperature is developed based on the input-output sampled data, which can avoid the internal complexity of DMFC stack. In the controlling process, with the network model trained well as the reference model of the DMFC control system, a novel fuzzy genetic algorithm is used to regulate the parameters and fuzzy rules of a neural fuzzy controller. In the simulation, compared with the nonlinear Proportional Integral Derivative （PID） and traditional fuzzy algorithm, the improved neural fuzzy controller designed in this paper gets better performance, as demonstrated by the simulation results.     

Effects of medium nutrition on cell growth and isocamptothecin A and B production by suspension cell culture of Camptotheca acuminata

The effects of initial sucrose concentration, nitrate to ammonium ratio, total N concentration and phosphate concentration in medium on cell growth and isocamptothecin A and B synthesis by suspension call culture of Camptotheca acuminata were investigated in 250 mL shake flasks. 30 g L^-1 sucrose concentration was beneficial to secondary metabolites synthesis. The cell growth and metabolites synthesis were also affected by the ratio of NO3^-/NH4^＋ , and nitrate was tavourable for cell growth. The maximum dry weight was achieved when nitrate was used as the sole N souree. The effect of total initial N on the cell cultures was also investigated with NO3^-/NH4^＋ ratio of 1 ： 2. The final dry cell weight was similar throughout culture period and 50 mM initial N was favourable for secondary metabolite synthesis. 50 mM initial phosphate concentration facilitated both cell growth and secondary metabolites synthesis.     

基于权矩阵低秩逼近的MIMO OTHR多模SDC抑制算法

扩展多普勒杂波是天波超视距雷达(over the horizon radar,OTHR)慢速舰船目标检测面临的关键问题。在新一代多输入多输出天波超视距雷达系统下,基于最小方差无失真响应(minimum variance distortionless response,MVDR)权矢量,提出一种权矩阵低秩逼近的多模扩展多普勒杂波抑制算法。利用阻塞矩阵进行数据预处理,并利用“发射〖CD*2〗接收”二维权矩阵的特征分解对双迭代MVDR算法进行了多级扩展,在减小了计算量和样本需求的基础上,进一步改善俯仰空域滤波的输出信杂噪比,提升OTHR对低可探测慢速舰船目标的检测性能。理论分析和仿真验证了算法的有效性。