Cd,Pb等7种元素在乌兰木伦河和窟野河底质中的分布规律

乌兰木伦河发源于内蒙伊克昭盟东胜附近矿区中部,在神木县境下石拉沟处与正北流入的牛川相汇,下称窟野河.窟野河出矿区(店塔)流经神木县城及其南部的纵横黄土沟壑区,最后汇入黄河,属黄河中游的一条较大支流.乌兰木伦河和窟野河在矿区内流长分别为75km 和20km.目前规划开发的神府一东胜矿区,绝大多数的井口工业广场、洗煤厂,机械修造厂和各种配套的工业设施及十余万人的生活居住区等均设置在乌兰木伦河和窟野河的两侧.这两条河流将成为矿区大量生活污水和各种工业废水的唯一纳污水体,弄清重金属在这两条     

线性谐振子的坐标-动量不确定关系

讨论了量子力学中的不确定性原理,定量计算了线性谐振子的坐标-动量之间以及动能-势能之间的不确定度,证明了它们满足一般的不确定关系,并澄清了一些有关不确定关系的错误理解和描述.     

远程城市地铁施工测量管理模式的研究

目的研究一种基于网络的信息化地铁施工测量管理模式。方法采用B/S模式构建远程城市地铁施工测量管理系统,划分出测量系统、监测系统、管理系统3个子系统,规范各单位的权限及其工作内容。结果实现了浏览器端的地图可视化查询与分析、数据更新、各标段测量成果的查询与报检等功能。结论远程城市地铁施工测量管理模式优于传统管理模式,达到了动态管理与办公自动化一体化,应用在西安地铁1号线的施工中,起到了良好的效果。     

基于创新能力培养的现代控制理论课程教学体系构建

以培养学生的创新能力为目标,构建现代控制理论课程的教学体系.改革教学内容,增加相应的MATLAB控制仿真知识点,在授课中全过程加入课程思政,激发学生的爱国情节,采用混合式教学模式,完善课程的考核方式.教学实践表明,采用此教学体系后,提高了学生的学习热情,学生能够积极主动地投入到教学环节中,可以将所学到的理论知识运用到工程实际中,提高了动手能力和创新能力.     

RNAi-mediated gene silencing in non-human primates

The opportunity to harness the RNA interference (RNAi) pathway to silence disease-causing genes holds great promise for the development of therapeutics directed against targets that are otherwise not addressable with current medicines. Although there are numerous examples of in vivo silencing of target genes after local delivery of small interfering RNAs (siRNAs), there remain only a few reports of RNAi-mediated silencing in response to systemic delivery of siRNA, and there are no reports of systemic efficacy in non-rodent species. Here we show that siRNAs, when delivered systemically in a liposomal formulation, can silence the disease target apolipoprotein B (ApoB) in non-human primates. APOB-specific siRNAs were encapsulated in stable nucleic acid lipid particles (SNALP) and administered by intravenous injection to cynomolgus monkeys at doses of 1 or 2.5 mg kg(-1). A single siRNA injection resulted in dose-dependent silencing of APOB messenger RNA expression in the liver 48 h after administration, with maximal silencing of >90%. This silencing effect occurred as a result of APOB mRNA cleavage at precisely the site predicted for the RNAi mechanism. Significant reductions in ApoB protein, serum cholesterol and low-density lipoprotein levels were observed as early as 24 h after treatment and lasted for 11 days at the highest siRNA dose, thus demonstrating an immediate, potent and lasting biological effect of siRNA treatment. Our findings show clinically relevant RNAi-mediated gene silencing in non-human primates, supporting RNAi therapeutics as a potential new class of drugs.     

A loss-of-function RNA interference screen for molecular targets in cancer

The pursuit of novel therapeutic agents in cancer relies on the identification and validation of molecular targets. Hallmarks of cancer include self-sufficiency in growth signals and evasion from apoptosis; genes that regulate these processes may be optimal for therapeutic attack. Here we describe a loss-of-function screen for genes required for the proliferation and survival of cancer cells using an RNA interference library. We used a doxycycline-inducible retroviral vector for the expression of small hairpin RNAs (shRNAs) to construct a library targeting 2,500 human genes. We used retroviral pools from this library to infect cell lines representing two distinct molecular subgroups of diffuse large B-cell lymphoma (DLBCL), termed activated B-cell-like DLBCL and germinal centre B-cell-like DLBCL. Each vector was engineered to contain a unique 60-base-pair 'bar code', allowing the abundance of an individual shRNA vector within a population of transduced cells to be measured using microarrays of the bar-code sequences. We observed that a subset of shRNA vectors was depleted from the transduced cells after three weeks in culture only if shRNA expression was induced. In activated B-cell-like DLBCL cells, but not germinal centre B-cell-like DLBCL cells, shRNAs targeting the NF-kappaB pathway were depleted, in keeping with the essential role of this pathway in the survival of activated B-cell-like DLBCL. This screen uncovered CARD11 as a key upstream signalling component responsible for the constitutive IkappaB kinase activity in activated B-cell-like DLBCL. The methodology that we describe can be used to establish a functional taxonomy of cancer and help reveal new classes of therapeutic targets distinct from known oncogenes.     

蔗糖、甜蜜素与白酒对白蚁引诱效应的分析

本文研究了蔗糖、甜蜜素和白酒对台湾乳白蚁和黄肢散白蚁的引诱效果.结果表明50%的蔗糖和酒精含量为40%的白酒对台湾乳白蚁具有较强的引诱效果,20%的甜蜜素对台湾乳白蚁具有轻微的引诱效果; 15%的蔗糖和酒精含量为50%的白酒对黄肢散白蚁具有一定的引诱效果,而甜蜜素对黄肢散白蚁基本没有引诱效果.     

Delta算子系统D稳定鲁棒容错控制

研究了Delta算子描述的线性系统基于圆形区域极点配置的鲁棒容错控制(定义作D稳定鲁棒容错控制)问题·利用线性矩阵不等式,给出了Delta算子描述的标称线性系统通过状态反馈实现D稳定容错控制的两个充分条件和D稳定容错控制器的设计方法·利用代数Riccati方程,给出了Delta算子描述的不确定线性系统D稳定鲁棒容错控制的充分条件和D稳定鲁棒容错控制器的设计方法·将连续与离散系统有关该问题的结果统一到了Delta算子框架中·     

基于非标记定量技术的肝细胞癌血浆蛋白质组学研究

采用非标记定量蛋白质组学技术获得10例肝细胞癌患者和10例健康人的血浆样本蛋白质组表达谱数据,并对其细胞定位与功能进行注释分析.利用修正t-检验算法对差异蛋白质进行筛选,通过DAVID平台分析软件对差异蛋白质进行功能注释分析,发现了一系列潜在的肝细胞癌血浆诊断生物标志物,包括16个肝细胞癌血浆上调蛋白质和15个下调蛋白...     

基于深度学习的多模态眼科图像回归预测

青光眼是世界第二大致盲性眼病，视网膜神经纤维层（RNFL）缺损是诊断青光眼的重要特征。在临床应用中主要采用光学相干断层扫描（OCT）测量RNFL厚度。然而在我国的多数中小型医院和体检中心，只有眼底照相机而不具备OCT设备。因此利用眼底照和OCT的多模态数据，设计了一种基于眼底照来预测RNFL厚度的深度残差回归神经网络。该网络通过眼底照中的局部区域信息预测此区域的RNFL厚度，并对视盘外围一周范围内的RNFL厚度给出全面的刻画。在一个来自北京同仁医院的真实数据集上的实验结果显示，本文算法预测的RNFL厚度值与OCT测量值具有高度的一致性（对于正常眼平均绝对误差E_MA=14.884，Pearson相关系数r=0.885，决定系数R²=0.781；对于青光眼E_MA=15.108，r=0.872，R²=0.754）。评估结果表明所提方法对基于眼底照预测RNFL厚度具有良好的临床实用性。