Faithful chaperones

This review describes the properties of some rare eukaryotic chaperones that each assist in the folding of only one target protein. In particular, we describe (1) the tubulin cofactors, (2) p47, which assists in the folding of collagen, (3) α-hemoglobin stabilizing protein (AHSP), (4) the adenovirus L4-100 K protein, which is a chaperone of the major structural viral protein, hexon, and (5) HYPK, the huntingtin-interacting protein. These various-sized proteins (102–1,190 amino acids long) are all involved in the folding of oligomeric polypeptides but are otherwise functionally unique, as they each assist only one particular client. This raises a question regarding the biosynthetic cost of the high-level production of such chaperones. As the clients of faithful chaperones are all abundant proteins that are essential cellular or viral components, it is conceivable that this necessary metabolic expenditure withstood evolutionary pressure to minimize biosynthetic costs. Nevertheless, the complexity of the folding pathways in which these chaperones are involved results in error-prone processes. Several human disorders associated with these chaperones are discussed.     

关于可测函数列的收敛性问题的教学研究

对实变函数课程中可测函数列的收敛性问题 ,从教材处理方面 ,提出了一些改进的意见 .     

软土地基上储油罐的抗震设计

软土地基上储油罐的抗震分析由于翘离等因素的非线性影响，计算非常繁复，虽然经过一系列简化，仍须经过迭代运算，该文根据储油罐的工程特点及震害经验提出了一种简化计算方法以方便工程应用。     

圆形管道振动分析及实验研究

应用摄动地和波动分析法研究圆形管道的振动；利用MASP编辑管道动态振型，通过实验对理论指导的正确性加以验证。     

局部压缩映射的不动点

通过构造反例指出文 [1 ]中关于紧度量空间中局部压缩映射有唯一不动点这一结论的错误 ,并证明了在紧度量空间上局部压缩映射的不动点或周期点的一定存在     

基于自组织特征映射的矢量量化方法

对自组织特征映射神经网络的特性进行分析，并将其与矢量量化问题的实质进行比较，提出了一个实现矢量量化的自组织特征映射算法。分析与实验表明，该算法是稳定收敛的。算法的学习结果与网络的初始状态无关，并且十分接近于全局最优解的下限。将该算法应用于图像数据的压缩，取得了很好的结果     

动态多媒体网页的制作及研究

利用现代技术，通过网页与数据库系统的连接，同时采用多媒体技术与网页技术相结合，创建新颖别致的动态网站，同时，开发了DHTML方式的课件制作技术及课件制作工具，特别是通过采取图像的可替代方式和重复使用等措施，解决了网页的多媒体视觉效果和下载速度这一矛盾。     

Germline-specific dgcr8 knockout in zebrafish using a BACK approach

Zebrafish is an important model to study developmental biology and human diseases. However, an effective approach to achieve spatial and temporal gene knockout in zebrafish has not been well established. In this study, we have developed a new approach, namely bacterial artificial chromosome-rescue-based knockout (BACK), to achieve conditional gene knockout in zebrafish using the Cre/loxP system. We have successfully deleted the DiGeorge syndrome critical region gene 8 (dgcr8) in zebrafish germ line and demonstrated that the maternal-zygotic dgcr8 (MZdgcr8) embryos exhibit MZdicer-like phenotypes with morphological defects which could be rescued by miR-430, indicating that canonical microRNAs play critical role in early development. Our findings establish that Cre/loxP-mediated tissue-specific gene knockout could be achieved using this BACK strategy and that canonical microRNAs play important roles in early embryonic development in zebrafish.     

The role of endosomal-recycling in long-term potentiation

Long-term potentiation (LTP) defines persistent increases in neurotransmission strength at synapses that are triggered by specific patterns of neuronal activity. LTP, the most widely accepted molecular model for learning, is best characterised at glutamatergic synapses on dendritic spines. In this context, LTP involves increases in dendritic spine size and the insertion of glutamate receptors into the post-synaptic spine membrane, which together boost post-synaptic responsiveness to neurotransmitters. In dendrites, the material required for LTP is sourced from an organelle termed the endosomal-recycling compartment (ERC), which is localised to the base of dendritic spines. When LTP is induced, material derived from the recycling compartment, which contains α-amino-3-hydroxy-5-methyl-4-isoxazole propionate-type glutamate receptors (AMPARs), is mobilised into dendritic spines feeding the increased need for receptors and membrane at the spine neck and head. In this review, we discuss the importance of endosomal-recycling and the role of key proteins which control these processes in the context of LTP.     

Co-substituted ATS-type molecular sieves and films for cyclohexane oxidation

Pure CoAPO-36 crystal grains were synthesized by hydrothermal reaction. Porous α-Al₂O₃, stainless steel and K₂Ti₆O₁₃ supported CoAPO-36 films were fabricated using in situ crystallization for the first time. SEM images and XRD measurements demonstrate that a continuous CoAPO-36 film was obtained on the K₂Ti₆O₁₃ substrate. Diffuse reflectance UV-Vis spectra indicate that Co²⁺ has been incorporated into the ATS framework. Both CoAPO-36 crystal grains and the K₂Ti₆O₁₃ supported CoAPO-36 film exhibit satisfactory catalytic activities for the oxidation of cyclohexane.