噬菌体展示禽流感病毒抗原变异性基因片段文库的构建

构建T7噬菌体展示禽流感病毒抗原变异性基因片段文库. 首先， 从Gene Bank中查找筛选禽流感病毒抗原变异性基因, 将其截短、 修饰、 简并后得到禽流感病毒抗原变异性基因微阵列. 其次， 将合成的禽流感病毒抗原变异性基因片段文库扩增、 酶切, 链接到双酶切后的T7噬菌体载体基因上, 构成重组噬菌体DNA. 最后， 重组噬菌体DNA经体外包装和扩增, 得到T7噬菌体展示文库, 并进行T7噬菌体展示文库滴度、 重组率和免疫活性测定. 实验结果表明, 从Gene Bank中查找、 筛选、 剪切和修饰共获得96 258条序列构建T7噬菌体展示文库, 原始文库滴度为3.6×107个菌落/mL, 重组率大于90%. 用禽流感病毒H5N1抗体进行捕获, 经聚合酶链式反应(PCR)鉴定, 得到理想目的条带, 证明噬菌体表面展示蛋白具有抗原活性, 可用于禽流感病毒感染患者的快速检测及抗原表位筛选.     

育种目标性状间的同一关系分析方法研究

借助集对分析理论,提出了一种新的育种目标性状间的关系分析方法,阐明了其基本原理与分析步骤,并结合小麦育种实例,介绍了这种分析方法的具体应用。在此基础上,讨论了这种分析方法的优越性及尚待改进之处。表3,参9。     

Genetic and photoperiodic control of an avian reproductive cycle

European blackcaps,Sylvia atricapilla, with one breeding season per year, have a single-peaked annual testes cycle. However, African conspecifics from the Cape Verde Islands with two breeding seasons per annum demonstrate a two-peaked cycle. Both population-specific cycles reflect differences in the respective endogenous circannual rhythms. Experimental hybridization of birds of the two populations resulted in an intermediate pattern of testes cycle, thus demonstrating that there are genetic components for some temporal aspects in an avian reproductive cycle. Another characteristic of the African birds, their extremely rapid juvenile development and early sexual maturity (at an age of 5–6 months) proved largely to be a photoperiodic (short-day) effect in birds hatched in autumn. The same effect could also be induced in European conspecifics exposed to correspondingly short day-lengths.     

Using genetically-defined rodent strains for the identification of hippocampal traits relevant for two-way avoidance behavior: a non-invasive approach

Summary Genetically-defined rodent strains permit the identification of hippocampal traits which are of functional relevance for the performance of two-way avoidance behavior. This is exemplified here by analyzing the relationship between infrapyramidal mossy fibers (a tiny projection terminating upon the basal dendrites of hippocampal pyramidal neurons) and two-way avoidance learning in about 800 animals. The necessary steps include 1) identification of structural traits sensitive to selective breeding for extremes in two-way avoidance, 2) testing the robustness of the associations found by studying individual and genetical correlations between hippocampal traits and behavior, 3) establishing causal relationships by Mendelian crossing of strains with extreme structural traits and studying the behavioral consequences of such structural randomization, 4) confirming causal relationships by manipulating the structural variable in inbred (isogenic) strains, thereby eliminating the possibility of genetic linkage, and 5) ruling out the possibility of spurious associations by studying the correlations between the hippocampal trait and other behaviors known to depend on hippocampal functioning.In comparison with the classical lesion approach for identifying relationships between brain and behavior, the present procedure appears to be superior in two aspects: it is non-invasive, and it focuses automatically on those brain traits which are used by natural selection to shape behaviorally-defined animal populations, i.e., it reveals the natural regulators of behavior.     

中国银杏种植业良种化进程的思路

阐述良种化的概念和意义;根据我国银杏生产发展的现状,提出中国银杏种植业现阶段良种化的进程是：加快产区良种选育和新发展区良种引种试验,加快良种苗木繁殖体系建设,用良种接穗改造低产劣种株,同时稳步发展良种苗种植;认为银杏在现阶段只宜采用科学的选择育种方式。     

灰胸竹鸡人工繁殖的研究

１９９５年１０月至１９９７年１０月对野生灰胸竹鸡（Ｂａｍｂｕｓｉｃｏｌａｔｈｏｒａｃｉｃａ）进行了人工繁殖的研究,结果为：产卵期４月下旬至７月下旬;卵的平均质量在（１５±２）ｇ之间;最佳孵化温度、相对湿度：１～１４ｄ内,温度为３８℃,相对湿度６５％～７０％;１５～１８ｄ内,温度降到３７℃,相对湿度７５％;雏鸟的生长模型为：ｍ＝２６２／（１＋ｅ２．６１－０．０３７ｔ）．     

白马鸡配对期两性行为的比较

2003年4月和2004年4月在四川省稻城县著杰寺采用瞬时扫描取样法对野生白马鸡(Crossoptilon crossoptilon)配偶行为进行了研究.结果表明,白马鸡配偶中雌、雄个体全天伴随活动,无特殊情况不会分离.在不受其他个体干扰的情况下,配偶移动时雌性多在前并控制移动方向.雌、雄鸟间距离平均为(1.75±0.03)m,在雄性驱逐其他个体时间距最大.在全天行为记录中,雄性警戒的时间最多,之后依次为取食、移动、驱逐、站立、休息和其他行为.雌性在一天中行为时间分配依次为取食、移动、休息、站立、警戒和其他行为.驱逐行为完全由雄性承担.两性共有行为中雄性用于警戒的时间显著高于雌性(P<0.05),用于取食、休息的时间显著低于雌性(P<0.05),用于移动和站立的时间与雌性差异不显著(P>0.05).     

生物技术在玉米育种中的应用

介绍了在玉米育种上得到应用并取得可喜进展、简便实用的主要生物技术。阐述了这些生物技术的操作方法，以及它们的现状和存在问题。     

河南郑州白鹭的繁殖习性

于 2 0 0 0～ 2 0 0 3年繁殖季节对河南郑州市区白鹭的生态习性进行了研究 ,结果表明白鹭于每年 4月上旬至 8月底在市区营巢繁殖 ,在营巢地居留期约 1 45 d.白鹭窝卵数为 5 .1 4± 0 .69( 4～ 6)枚 ,出雏数为4.43± 1 .2 7( 2～ 6)只 ,孵化期为 2 5 .1 4± 0 .69( 2 4～ 2 6) d.孵化率为 86.1 1 % ,育雏期约 42 d,雏鸟滞留巢区比荒野地长 1 0 d左右 .雏鸟 2 0日龄统计成活率为 87.1 0 % ,雏鸟死亡原因多为恶劣气候条件所致 ,如大风、暴雨等