富硒酵母的筛选

对 5属 7种 30株酵母菌进行了富硒酵母的筛选 ,经过抗性筛选和摇瓶发酵培养获得 NK2和NK4两株富硒菌株 ,在 YEPD培养基 ,培养 2 0 h加硒 ,4 8h收获 ,富硒菌株的含硒量达到 80 0 μg/g干菌体。     

用玉米秸秆生产单细胞蛋白的菌种选育及发酵工艺

通过硫酸常温酸解玉米秸秆粉,以正交试验优选发酵菌株、培养基组成及培养条件.实验表明,采用绿色木霉(Trichodermaviride)3.2774,(NH4)2SO425 g/L,酸解秸秆200 g/L,温度30℃,250 mL摇瓶装液量30 mL,搅拌转速200 r/min时,还原糖得率最高.以绿色木霉3.2774为出发菌株,经紫外诱变、糖化实验、稳定性实验等选育出绿色木霉NUA-051菌株,传代8次,发酵糖化率为40.7%~45.3%,具有遗传稳定性.以蛋白质得率为目标,通过5 L发酵罐正交试验确立了以酸解玉米秸秆为原料生产单细胞蛋白的发酵工艺,即酸解玉米秸秆150 g/L,木霉发酵48 h,接种酵母量2%,(NH4)2SO415 g/L,KH2PO46 g/L,MgSO4.7H2O0.4 g/L,通风量4 L/(L.min),搅拌转速500 r/min,pH值5,温度35℃.木霉发酵时间40 h,酵母发酵时间24 h,发酵总周期64 h.     

杨树木屑废弃物酶解液发酵制备富硒酵母的研究

以杨树木屑为原料,考察了固液比、酶添加量、pH和酶解时间对其酶解效果的影响。通过优化酶解条件制得葡萄糖质量浓度为55 g/L的酶解液,将酶解液作为碳源替代传统培养基中的葡萄糖,其他营养成分不变,发酵制备富硒酵母,重点研究了酵母菌在酶解液中的生长情况和富硒能力。结果表明,酵母在酶解液培养基中长势良好,5 L发酵罐发酵36 h后,每L发酵液酵母干重23.9 g,单位酵母硒含量超过3 500 μg/g。     

米根霉、法夫酵母双菌共发酵淀粉生产虾青素的初步研究

对米根霉、法夫酵母共发酵淀粉生产虾青素进行了研究．摇瓶试验结果表明，米根霉、法夫酵母共发酵淀粉生产虾青素适宜的培养基为淀粉50g／L、1gKH2PO4/L、1g CaCl2／L、1g酵母膏/L、0.5gMgSO4／L、5g（NH4）2SO4/L，控制培养基初始pH为8．0、发酵温度为22℃及同时接种米根霉和法夫酵母有利于虾青素的合成，5L罐试验结果表明虾青素合成滞后于糖的利用，且分成两个增长阶段，虾青素的最大产量达2．48mg／L．这些试验结果不仅证实了共培养米根霉和法夫酵母发酵淀粉生产虾青素的理论可行性，而且为进一步利用淀粉为原料生产虾青素提供了试验参考．     

戊糖己糖混合糖发酵生产乙醇的主要影响因素

以树干毕赤酵母为发酵菌株,高混合糖(木糖、葡萄糖)为发酵底物,确定树干毕赤酵母高糖浓度发酵时所需的条件.研究结果表明,在高糖浓度乙醇发酵中,树干毕赤酵母较为适宜的发酵温度为30℃,发酵24 h,残糖浓度和乙醇浓度分别为0.1 g/L和32.5 g/L.添加硫酸铵1.1 g/L+微量元素发酵效果较好,乙醇浓度为33.2g/L.由典型发酵过程中各物质浓度变化曲线可知,酵母优先利用葡萄糖,发酵12 h葡萄糖和木糖的还原糖利用率分别为89.3%、10.7%.待12 h葡萄糖几乎被消耗完后,对木糖的利用开始占主导地位,乙醇浓度随着糖的不断消耗而逐渐提高.发酵28h时乙醇浓度最高,达到33.2 g/L.     

固定化酵母流化床生物反应器发酵玉米淀粉的研究

对固定化酵母流化床生物反应器发酵玉米淀粉进行了研究.结果表明,在南阳混合酵母、拉斯12号酵母以及古巴Ⅱ号酵母3种酵母中,南阳混合酵母最适合作为固定化的菌种.发酵醪初糖为9 86～15 88°Bx,发酵时间为6～11h,成熟发酵醪中酒精的体积百分比含量达4 8%～9 2%,反应器的乙醇生产能力为3 8～4 5g/(L·h),凝胶的乙醇生产能力为11 3～13 6g/(L·h),残糖均低于1 0°Bx.     

毕赤酵母发酵甘露醇产乙醇的条件研究

在250ml三角瓶中进行毕赤酵母（Pichia angophorae）发酵不同浓度的甘露醇生产乙醇的试验。试验先以浓度为20g/L、40g/L、60g/L、80g/L的甘露醇进行发酵试验确定出最适培养基组分,然后以正交试验确定菌株的最优发酵条件。结果确定出最适的发酵培养基组分为：甘露醇20g/L,酵母浸粉0.3g/L,麦芽浸粉0.3g/L,（NH4）2SO45g/L,KH2PO42g/L,MgSO4·7H2O 0.4g/L。最佳发酵条件为：温度32℃,摇床转速150rpm,初始pH值4.5,发酵液体积150ml,乙醇最大产量为0.45g ethanol/g mannitol。     

Galactomyces reessii转化β-甲基丁酸生产β-羟基-β-甲基丁酸

Galactomycesreessii可将 β-甲基丁酸 ( MBA)转化为 β-羟基 - β-甲基丁酸 ( HMB)。摇瓶实验表明发酵过程中提供生长因子的酵母萃取物浓度以 3 g/L较适宜。高浓度钠、铵离子对菌体生长有抑制作用 ,为保证转化 MBA所需 p H可采用混合碱 ( Na OH、KOH、NH4 OH)来调节 ,以防止单一阳离子浓度过高对菌体生长和产物形成的抑制。MBA对菌体生长有明显的抑制作用 ,1 0 g/L的MBA可使菌体浓度减少 3 0 %。分批补料发酵中 ,连续流加葡萄糖和 MBA,控制发酵液中葡萄糖浓度不超过 3 g/L,MBA浓度不超过 4g/L ,1 0 2 h时 HMB浓度达 2 9.0 g/L ,转化率达 5 7.3 % ,HMB平均生产率达 0 .0 98g/( L· h)。     

发酵抑制物对树干毕赤酵母戊糖发酵的影响

研究了常见发酵抑制物(甲酸、醋酸和乙醇)对树干毕赤酵母(Pichia stipitis)P2生长和发酵木糖的影响.结果表明:树干毕赤酵母P2具有良好的抗发酵抑制物能力.当发酵液中含有4.0 g/L的醋酸时,树干毕赤酵母P2生长和发酵木糖情况良好;当发酵液中含有5.0g/L的甲酸时,树干毕赤酵母P2对木糖的利用率降低,但对酵母生长的影响并不明显;另外,树干毕赤酵母P2耐受乙醇的浓度约为40.0 g/L.     

富硒鸡腿菇菌丝体深层发酵培养基的优化

研究营养因子对鸡腿菇菌丝体生长和富硒的影响,筛选到适合鸡腿菇富硒深层发酵的优化培养基配方为:葡萄糖2%,玉米粉4%,花生粕0.4%,KH2PO4 0.2%,MgSO4·7H2O0.1%,VB1 10×10-3 g/L,Na2SeO3 4.4×10-3 g/L.在此优化培养基的基础上进行5 d的摇瓶发酵培养,得到菌丝体生物量为18.14 g/L,富硒率为56.35%.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.