16种食用菌超氧物歧化酶(SOD)活性、热稳定性及同工酶的研究

对16种新鲜食用菌SOD活性、热稳定性及同工酶类型进行研究,结果表明,大多数食用菌有较高SOD活性,其中以金顶侧耳的酶活性最高,达1622U/g,比活性也有366U/mg,酶活性超过1000U/g的有糙皮侧耳、蘑菇、草菇和柳松菇,有14种食用菌SOD活性超过500U/g.食用菌中主要含对氯仿乙醇液敏感的SOD,占其总活性的70%~96%,以金顶侧耳和糙皮侧耳的活性最高,分别为1538U/g和1052U/g.SOD的热稳定性研究表明,大多数食用菌SOD在90℃加热10min后,能保留40%以上的活性,金针菇、滑菇、毛头鬼伞和大球盖菇的菌盖在90℃加热10min后,能保留75%以上的活性.食用菌SOD类型为Mn-SOD,未发现有Cu·Zn-SOD.菌盖和菌柄的分析结果表明,二者在SOD活性、热稳定性及同工酶谱带数方面存在一定的差异.     

活性干酵母富硒培养条件的研究

以安琪活性干酵母为出发菌种,分析了培养基初始硒浓度、接种量、装液量、温度、初始pH以及转速等发酵条件对酵母生物量及富硒量的影响,并通过正交试验设计初步确定了培养的最优方案.在最佳的摇瓶培养条件下(初始硒浓度25μg/mL,接种量10%,装液量60/250 mL,温度30℃,初始pH 5.0,摇床转速160 r/min,培养40 h后),该活性干酵母的生物量及富硒量分别达到9.89 g/L、954 μg/g.     

大球盖菇黄酮类化合物提取及抑菌性研究

为了探讨大球盖菇黄酮类化合物提取工艺及其抑菌效果,通过正交试验确定了大球盖菇黄酮类化合物的优化提取工艺条件;采用大球盖菇黄酮类化合物对大肠杆菌、青霉菌、啤酒酵母进行抑菌试验.结果表明,优化后的提取工艺为:乙醇体积分数95%,物料比为1∶90,温度60℃,时间120 min,提取率为7.94‰.40 g/L的大球盖菇黄酮类化合物溶液对大肠杆菌和青霉菌有抑制作用;20 g/L和10 g/L的溶液只对大肠杆菌有抑制作用,对青霉菌没有抑制作用;大球盖菇黄酮类化合物对啤酒酵母没有抑制作用;对大肠杆菌的抑制作用要强于对青霉菌的抑制作用.     

天然富硒绿茶醇溶性活性物质提取条件优化

以天然富硒绿茶为原料,采用超声波辅助提取醇溶性活性物质,通过单因素实验和正交试验优化了提取条件.结果表明最佳提取条件为:乙醇体积分数50%,料液比1∶25,提取温度60℃,时间2h,超声波功率400W,提取得率为44.44%.制得的富硒绿茶活性物质化学组成为:硒1.03μg/g,茶蛋白31.09%,茶多酚53.22%,茶多糖13.56%.     

富硒鲍鱼菇的深层培养及其特性

报导在含有不同浓度无机硒的液体培养基中培养鲍鱼菇菌丝体，探讨其耐硒和富硒特性。结果表明：鲍鱼菇有较强的耐硒特性和很强的富硒能力，鲍鱼菇菌丝体硒含量随培养基质硒浓度的增加而增高，在培养基中添加硒量为５μｇ／ｍｌ时，富硒鲍鱼菇菌丝体含硒量可达２００μｇ／ｍｌ时，为对照的４０倍，硒回收率达３８．８％，其氨基酸及多糖含量均高于对照。     

苦参碱和氧化苦参碱的抑菌活性及增效作用

研究了苦参碱、氧化苦参碱对几种林木病原真菌孢子萌发和菌丝生长的抑制活性.结果表明苦参碱和氧化苦参碱对供试林木病原真菌孢子萌发均有抑制作用,其中苦参碱对杨褐斑病菌和龙竹材霉变菌抑制作用较强,致死中量EC50分别为123、272 μg/mL.氧化苦参碱对杉木猝倒病菌抑制作用最强,EC50为26μg/mL,活性比供试的75%百菌清可湿性粉剂(EC50=91μg/mL)和70%甲基托布津可湿性粉剂(EC50=283 μg/mL)的活性高.苦参碱和氧化苦参碱对供试林木病原真菌菌丝生长有抑制作用,其中苦参碱对松枯梢病菌抑制作用较强,EC50为428μg/mL;氧化苦参碱对华山松腐烂病菌的抑制作用较强,EC50为323μg/mL.以苦参碱(氧化苦参碱)与合成农药按1:5质量比混合进行林木病原真菌菌丝生长抑制增效试验,结果表明混剂的EC50均低于34 μg/mL,共毒系数明显大于100,对华山松腐烂病菌具显著增效作用,对杉木猝倒病菌具不同程度的相加或增效作用.     

高效液相色谱-氢化物发生原子荧光光谱法测定食品中多形态硒含量

研究基于高效液相色谱-氢化物发生原子荧光光谱建立了一种适用于富硒蛋白粉、富硒大米、富硒香菇和富硒酵母等富硒食品中硒酸根、硒代胱氨酸、甲基硒代半胱氨酸、硒代蛋氨酸的检测方法。样品经Tris缓冲液提取,蛋白酶和胰蛋白酶酶解,以V(20mmol/L磷酸氢二胺水溶液,pH=6.0)∶V(甲醇)=98∶2为流动相,经Hamilton PRP-X100阴离子交换色谱柱分离测定。结果表明,4种硒形态化合物在5~100μg/kg范围内线性良好(R²＞0.999),方法检出限可达25~50μg/kg,平均加标回收率为81.37%~93.80%,且RSD为2.80%~7.22%。应用此方法对4种市售富硒食品(富硒蛋白粉、富硒大米、富硒香菇和富硒酵母)中4种硒形态化合物进行检测,前处理步骤简单,测定快速、准确度和灵敏度高,为食品中硒形态评价和质量控制提供了方法和依据。     

黑木耳富硒发酵条件的优化

为获得富硒黑木耳菌丝体,以亚硒酸钠为硒源对黑木耳(Auricularia auricular)高产菌株G6进行富硒发酵条件优化.以菌丝体性状和菌丝体硒含量为指标考查不同硒质量浓度对其生长的影响,并确定最适硒质量浓度;通过单因素和正交实验对G6菌株液体富硒发酵条件进行优化.结果,培养基中硒质量浓度＜50 mg/L时,菌丝体的硒含量随硒质量浓度的提高而提高;硒质量浓度为50 mg/L时,菌丝体硒含量最高,为8.5 μg/g;硒质量浓度＞50mg/L时,硒含量不升反降.表明适量亚硒酸钠有利于菌丝体对硒的吸收和利用,过量添加时则抑制菌丝体生长,不利于菌丝体对硒的吸收和利用.优化后的富硒黑木耳G6菌株液体发酵培养条件为硒质量浓度50mg/L,玉米粉15 mg/L,豆饼粉20 mg/L,装液量200 mL/500 mL三角瓶,转速160 r/min,25℃发酵培养6d.发酵液中菌丝体硒质量分数达到9.2 μg/g,发酵液多糖质量浓度可达3.7 g/L.     

常压室温等离子体诱变选育富硒纳豆芽孢杆菌

纳豆芽孢杆菌可以转化亚硒酸钠为有机硒。对一株纳豆芽孢杆菌的生长曲线进行测定,确定了亚硒酸钠适宜的添加时间和添加量。以纳豆芽孢杆菌BSN424为出发菌株,采用常压室温等离子体诱变系统进行诱变,根据耐硒和富硒能力筛选,经连续传代培养后筛选出了富硒纳豆芽孢杆菌。结果表明,适宜加硒时间为培养后3h,培养时间为24h,培养基适宜硒质量浓度为6μg/mL,常压室温等离子体诱变系统功率为100W,诱变时间为25s。诱变后筛选得到一株具有较高富硒能力的诱变菌株BN-44,经摇瓶发酵后的富硒量为1136.43μg/g,相比出发菌株的742.12μg/g提高了53.13%。研究表明常压室温等离子体诱变育种能有效地对纳豆芽孢杆菌BSN424进行诱变,旨在为有机硒生物转化法中寻找益生菌富硒载体及其诱变育种提供一定依据。     

微波消解-紫外分光法测定富硒沼泽红假单胞菌中的硒含量

对富硒沼泽红假单胞菌菌体中微量元素硒含量的测定方法进行了研究,采用微波消解-紫外分光光度法测定了样品中的硒含量,对微波消解样品和紫外分光光度法的各种操作条件进行了优化,得到的优化条件是:消解液为HNO₃/H₂O₂ 5:1（V/V）,微波功率为650 W,消解时间为3 min,测定波长为334 nm,配位剂用量为3 mL,配合反应酸度及萃取酸度为2.0,反应温度为45℃,反应时间为50 min.在优化条件下,该方法的测定范围为1～200μg（y=0.0104x+0.0024,r=0.9997）,检出限达0.91μg,相对标准偏差为1.76%,回收率为98.4～103.1%.该方法具有灵敏度高、稳定性好、操作简单等显著优点,适于基层实验室和工业生产中应用.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.     

Tracking data management using topology of moving object database system for moving object constrained trajectory in LBS application

Advances in wireless technologies and positioning technologies and spread of wireless devices, an interest in LBS (Location Based Service) is arising. To provide location based service, tracking data should have been stored in moving object database management system (called MODBMS) with proper policies and managed efficiently. So the methods which acquire the location information at regular time intervals then, store and manage have been studied. In this paper, we suggest tracking data management techniques using topology that is corresponding to the moving path of moving object. In our techniques, we update the MODBMS when moving object arrived at a street intersection or a curved road which is represented as the node in topology and predict the location at past and future with attribute of topology and linear function. In this technique, location data that are corresponding to the node in topology are stored, thus reduce the number of update and amount of data. Also in case predicting the location,because topology are used as well as existing location information, accuracy for prediction is increased than applying linear function or spline function.