脱脂豆粕中提取大豆异黄酮的条件研究

通过溶剂对比实验及单因素实验和正交实验，确定了从脱脂豆粕中提取大豆异黄酮的最佳提取溶剂及最佳提取条件．最佳提取条件为：乙醇浓度60％，提取温度70℃，物料比1：18，提取时间2h，提取次数2次．采用该方法从脱脂豆粕中提取大豆异黄酮的提取率为0．45％．     

大豆中异黄酮提取条件及其在提取液中的存在形式

以大豆豆粕为原料，以总异黄酮为目标产物。采用正交试验设计探讨了用乙醇—水体系作溶剂提取大豆异黄酮的最佳条件，即体积分数为70％的乙醇，m乙醇：V水=0．1g／ml。50℃下提取3次，每次回流时间1．0h。采用酸水解法和等电点沉淀法研究了异黄酮在提取液中的存在形式。即水不溶性异黄酮占8.0%，水溶性异黄酮占92．0％．其中与糖结合的占61.3％，与蛋白质结合的占30．7％。为进一步分离异黄酮提供了试验基础。采用HPLC法测定异黄酮的量。     

葛根提取物中异黄酮的测定

用薄层层析法对陕西太白产野葛根提取物中的异黄酮进行了定性检测,确定了葛根素（Ｒｆ＝０．２１）、大豆甙（Ｒｆ＝０．３４）、大豆甙元（Ｒｊ＝０．５６）、芒柄 花素（Ｒｆ＝０．７６）的存在;在测定波长为２４９ｎｍ条件下,建立了测定葛根提取物中异黄酮含量的柱层析、紫外分光光地,线性回归方程Ａ＝０．０４６＋９２．５５Ｃ（ｒ＝０．９９９８）;平均回收率为９９．１％（ＲＳＤ＝０．７１）。     

大豆异黄酮提取及纯化分离工艺探索

通过溶剂提取、浓缩、离心，然后进行层析分离，从大豆原料中提取及纯化分离大豆异黄酮，大豆异黄酮质量分数由原料中的0．0955％增加为提取液的0．582％，层析分离后达到8．23％，含量测定用HPCL法。     

均匀设计优化大豆异黄酮的超临界流体萃取

采用CO2超临界流体法提取大豆中异黄酮类成分.在单因素实验的基础上,利用均匀设计法考察萃取压力、萃取时间、萃取温度及药材粒度等4因素对大豆异黄酮提取率的影响,确定了CO2超临界流体法提取大豆异黄酮的最佳工艺为萃取压力45 MPa、萃取温度50 ℃、萃取时间4 h、药材粒度40目,在此工艺条件下进行实验,大豆异黄酮的提取率为(0.361±0.012)%.     

大豆天然甾醇的提取

用皂化法研究了7种不同原料中大豆甾醇的提取量，实验表明大豆油脱臭馏出物（简称DD油））是大豆箱醇提取的理想原料，对皂化法从DD油中提取大豆甾醇的实验条件进行正交优化，最佳实验条件是：400gDD油，500mL乙醇（95％），50gKOH（82％），回流皂化2h．在此条件下．粗甾醇的产量为26.0g，纯度为47.5％．     

超声波法从酱油渣中提取大豆异黄酮工艺条件研究

大豆异黄酮是大豆中的一类重要生理活性物质.近些年来,随着超声技术在异黄酮提取中的应用,其高效率、零污染的优点受到越来越多学者的关注.本文采用超声波结合乙醇法从酱油渣中提取大豆异黄酮,分别以乙醇浓度、料液比、提取时间、温度、提取次数、超声频率为单因素进行实验.结果表明,最佳的提取工艺条件是乙醇浓度为40%、料液比1∶50、温度30 °C、提取时间60 min、提取4次.     

大豆甙元磺酸盐对豚鼠离体回肠收缩的影响及途径分析

实验采用豚鼠回肠离体肠段，置于装有Krebs液的恒温灌流浴槽中，记录肠段收缩活动，探讨大豆甙元对消化道平滑肌的作用及其作用机理．结果表明，大豆甙元磺酸盐对豚鼠离体回肠收缩有明显抑制作用，纳洛酮能减弱大豆甙元磺酸盐对回肠的抑制作用，心得安、酚妥拉明、阿托品均不影响大豆甙元磺酸盐对回肠平滑肌的作用．实验结果提示，大豆甙元能抑制回肠平滑肌的收缩活动，其作用部分由μ-受体介导．     

超声波辅助提取大豆异黄酮生产工艺条件优化

探讨用超声波法提取大豆中异黄酮的工艺,大豆粉过40目筛,以石油醚脱脂后通过单因素实验和正交试验改变不同的提取工艺选取最佳提取条件。结果表明超声波辅助提取大豆异黄酮是行之有效的方法,影响提取的主次因素为乙醇浓度>料液比>温度>时间。最佳提取条件为:提取时间40 min,料液比1∶30(g.mL-1),乙醇体积分数60%,温度70℃。     

酱油渣中大豆异黄酮动态逆流提取及分离纯化的研究

以酱油渣干粉为原料,对大豆异黄酮提取条件和纯化方法进行研究.采用实验室模拟动态逆流提取酱油渣中异黄酮,并通过正交设计对大豆类黄酮提取条件进行了优化,确定了大豆类黄酮的最佳提取条件为80%乙醇, 提取前的浸泡时间12h,料液比1:10,得到总异黄酮提取率达0.35%,粗提物的纯度为2.01%.选取3种极性不同的大孔树脂填装制备柱,并利用中低压色谱对大豆异黄酮样品进行纯化.结果表明,NKA9大孔树脂对总大豆异黄酮的纯化效果最好,在60%乙醇洗脱时,产品纯度可达到32%.该方法能实施在短时间内对大豆异黄酮样品进行大量的纯化,满足大豆异黄酮产品生产的需要.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.