香菇菌深层发酵生产鸟苷酸试验

报道了香菇菌深层发酵生产鸟苷酸试验结果。以香菇菌Ｃｒ－０２１为材料，经多组试验结果分析。得出鸟苷酸产量与菌丝全干重成正比关系。应用Ｌｇ（３＾４）正交试验，得出培养基配方，在此基础上，设计了Ａ，Ｂ，Ｃ等３种培养基，并进行了发酵分析，其中Ｂ产鸟苷酸含量为２．１ｇ／Ｌ，氨基氮０．２ｇ／Ｌ。     

虎奶菇Pleurotus tuber-regium深层发酵的研究

报道了虎奶菇Pleurotus tuber-regium深层发酵的最适培养基和摇瓶发酵条件.试验结果表明,Pleurotus tuber-regium对单糖、双糖、多糖和糖醇都有一定的利用,但葡萄糖、麦芽糖等对菌丝生长效果较好;酵母粉、黄豆饼粉等蛋白质含量高的氮源对菌丝的生长较为有利;通过k9(34)正交试验确定了Pleurotus tuber-regium发酵的最适培养基为:5%葡萄糖,0.4%酵母粉.0.1%KH2PO4,0.3%MgSO4·7H2O,0.001%VB1及微量NaCl;一定浓度内Na+对茵丝体生长有促进效果.最适的摇瓶发酵条件为:培养基起始pH7.0,培养温度32℃,接种量15%,摇床转速150r/min,250mL三角瓶最适装液量为70mL,发酵周期为6d.     

茶薪菇液体发酵条件的研究

采用液体摇瓶培养法,对茶薪菇液体发酵的培养基pH、最适温度、摇瓶装量、摇瓶转速及不同液体培养基配方进行了探讨,结果表明,茶薪菇液体发酵的培养基初始pH为6.0-6.5,最适温度为25℃,摇瓶装量为50-70ml/250ml三角瓶,摇瓶转速为120-150rpm,最适培养基配方为以麸皮为主要成分的培养基。     

灵芝液体发酵培养条件的研究

灵芝的菌丝可获布于整个液体之中,所以菌丝发育比在固体培养基上迅速。我们采用液体摇瓶培养方法,对灵芝液体发醇培养的适宜温度,摇瓶装置,摇瓶振荡频率,培养基初始ＰＨ值进行了探讨,结果表明：灵芝液体发酵的适宜温度为２６℃,摇瓶装置为１２０ｍｌ／５００ｍｌ三角瓶,摇瓶振荡频率为１００－１２０次／分,培养基初始ＰＨ值为６．０－６．５。     

桐粕降解菌DBTM6培养条件及降解酶活性的研究

采用单因素试验法对桐粕降解菌DBTM6菌发酵的主要影响因素碳源、氮源、温度、转速、初始pH值等进行了研究,通过正交试验对其发酵培养基在摇瓶阶段进行了优化,并在优化后的条件下测定了该菌的生长曲线及其产生的蛋白酶、果胶酶和纤维素酶的活性.结果表明:该菌株最适培养基各成分质量分数为:玉米粉2.0%,蛋白胨0.5%,NaCl 0.5%,最适条件:温度为40℃,pH为6.0,转速为200 r/min.在最适培养条件下测定了DBTM6菌的纤维素酶活力为3.5717 U/mL,果胶酶活力为1.1415 U/mL,蛋白酶活力为36.358 3 U/mL,比活力分别为13.8545U/mg,4.4279 U/mg,141.0331 U/mg.     

发酵参数对产表活微生物H菌发酵液表面张力的影响

采用摇瓶发酵的方法研究了发酵条件对产表活微生物H菌的发酵液表面张力的影响。结果表明：发酵条件对发酵液表面张力的影响较大。接种用100ml三角瓶,2%的菌量,在28℃、150r/min的条件下,发酵液的表面张力较其他的发酵条件小。培养时有无光照和培养基的种类对发酵液的表面张力没有显著影响。     

绣球菌深层发酵工艺条件的研究

通过单因子试验和多因子正交试验,优化筛选了适于绣球菌摇瓶培养条件。结果表明,适于产菌丝体的摇瓶条件为：培养温度28℃,培养基起始pH4．5,接种量8％,摇瓶转速100r/min;摇瓶培养适于产菌丝体的最适碳氮源及其浓度分别为：可溶性淀粉2．5％,蛋白胨0．15％。适于产胞外多糖的摇瓶条件为：培养温度29℃。培养基起始pH5．5,接种量8％,摇瓶转速100r／min;摇瓶培养最适碳氮源及其浓度分别为：可溶性淀粉2．5％,蛋白胨0．25％。     

L-乳酸米根霉发酵条件优化研究

运用正交试验设计理论,对高产L-乳酸突变株NAF-032的最佳摇瓶发酵条件进行了研究,确定了最佳的发酵培养基和发酵条件.最佳发酵培养基为(g/L):葡萄糖160,氯化铵2,KH2PO4 0.3,MgSO4·7H2O 0.25,ZnSO4·7H2O 0.08.最佳培养条件为:34℃,摇床转速200 r/min,250 mL三角瓶装培养液50 mL,一次性添加CaCO380 g/L用于调节pH值.在最适发酵条件下,米根霉NAF-032的摇瓶发酵产L-乳酸产量达123.3 g/L.     

庆大霉素产生菌的紫外线诱变育种及发酵条件的研究

以庆大霉素产生菌ＪＹ１－１２为出发菌株，经紫外线照射，选育出性能优良菌株ＪＹ３－５，经正交试验确定了其最佳发酵培养基，研究了最佳发酵温及初始ＰＨ值，使其摇瓶效价提高３４．３％；３０ｍ＾３罐放大试验，比出发菌株效价提高１８．６％。     

内生真菌Hd3菌株发酵条件优化研究

利用Plackett-Burman设计和响应面设计方法对Hd3菌株的发酵条件进行了优化研究,确定了最佳培养基组成及培养条件.试验结果表明;Hd3菌株的摇瓶培养基配方为葡萄糖24.71 g/L,MgSO4·7H2O 1.08 g/L,土豆244.17 g/L.发酵条件为pH 6～7,250 mL三角瓶装90 mL发酵液,接入9×103 cfu/mL菌量,31℃培养9 d.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

The Analects and the Moment in Peking——The influence of a Confucian education system on the personality formation,the way of thinking and the fate of the main characters

0.Preface China has long been boasting of its colorful and immense culture which comprises of Taoism,Confucianism and Buddhism.The interwoven influence among the religions on Chinese way of thinking,on the nation＇s behavior in their social life throughout the long history is beyond list.