一品红离体培养快繁研究

以观赏花卉一品红的茎段为外植体，接种于添加不同浓度激素配比的MDS培养基上，进行离体培养。实验结果表明：诱导愈伤组织形成且频率最高的培养基是MS＋6－BA2．0＋NAA0．5（单位均为mg/L)；由愈伤 组织分化不定芽的最适培养基也是：MS＋6－BA2．0＋NAA0．5；当苗长到3－4cm时，移到1／2MS＋NAA0．2培养基上，生根效果最佳，用复合型营养土培植，移栽成活率高。     

叶用莴苣组织培养研究

以叶用莴苣(LactucasativaL)栽培品种的幼嫩叶片为实验材料,对叶用莴苣的组织培养进行了研究,探讨愈伤组织的形成、愈伤组织分化成芽和生根的条件.结果如下6BA和NAA的浓度影响叶用莴苣愈伤组织的形成、分化和生根培养,诱导愈伤组织的最适培养基为MS+6BA(0.5mg/L)+NAA(0.1mg/L),最适分化培养基为MS+6BA(0.5mg/L)+NAA(1.0mg/L),最适生根培养基为1/2MS+NAA(0.1mg/L).     

薄荷愈伤组织诱导与分化研究

本文研究了薄荷愈伤组织诱导与分化的影响因素。以薄荷茎段为外植体,研究培养基、激素水平对愈伤组织诱导、增殖培养的影响;以叶片为外植体,探讨叶片的愈伤组织诱导;以茎段、叶片和叶柄为外植体材料,比较不同外植体的愈伤组织诱导率。实验结果表明:MS比1/2 MS、B5培养基更适于茎段的诱导培养,诱导率达80%;叶片愈伤组织的诱导率为100%,以MS+1.0 mg/L BA+0.1 mg/L KT+0.5 mg/L NAA为最佳诱导培养基;幼叶是不同外植体中诱导效果最好的,以MS+0.5 mg/L BA+0.1 mg/L NAA为最佳诱导培养基。     

多因子正交试验对长春花离体培养条件的筛选

应用正交设计法考察了植物生长物质、蔗糖对长春花愈伤组织诱导、丛生芽诱导和生根培养的影响。最佳愈伤组织诱导培养基为MS＋2，4－D0.8mg/L NAA 1mg/L ZT 0.2mg/L。丛生芽诱导最佳培养基为MS＋6－BA0.2mg L NAA 0.3mg/L＋蔗糖30g/L，最佳生根培养基为1／2MS＋NAA 0.2mg/L IBA 0.2mg/L 蔗糖30mg/L。     

羽衣甘蓝无性系的建立

以羽衣甘蓝的嫩茎为实验材料，在MS＋BA2mg/L＋2，4－D0.5mg/L的培养基上可诱导出愈伤组织，在MS＋BA0.5mg/L IAA0.2mg/L的培养基上愈伤组织的分化率达13.2倍。分化苗在1／2MS培养基上生根率为96.7％；无菌茎段在1／2MS培养基上可直接获得生根苗，生根苗在河砂或炉灰渣上移栽和扦插的成活率为83.3％或86.7％。本实验羽衣甘蓝的繁殖速度可达每月13倍左右，这种繁殖速度可满足生产上的需要。     

外源激素对龙葵愈伤组织形成和器官发生的影响

龙葵不同部位的外植体在愈伤组织形成过程中所需的外源激素浓度不同。茎尖外植体在附加6—BA0.1—2.0(mg/L)、NAA0.1—2.0(mg/L)的Ls培养基上可高频率地诱导出愈伤组织。培养基中只加6—BA(2.0mg/L)或NAA(2.5mg/L)一种外源激素也可诱导出愈伤组织,但诱导所需的时间较长。较高的6—BA浓度(2.0mg/L)有利于子叶外植体形成愈伤组织,较高的NAA(2.0mg/L)有利于胚轴形成愈伤组织。器官分化过程决定于诱导愈伤组织时培养基中的激素成分。     

两种补血草属植物愈伤组织诱导的比较

以黄花补血草和大叶补血草无菌苗叶片为外植体,采用不同激素配比诱导愈伤组织,并分化获得不定芽,以建立两种补血草无性系,为植株快繁和细胞悬浮培养提供理论依据.结果表明:以补血草无菌苗叶片为外植体进行愈伤组织诱导和分化,可实现补血草快速繁殖,黄花补血草愈伤组织的诱导和分化较大叶补血草的容易.黄花补血草最适愈伤组织诱导培养基为MS 6-BA(0.3～0.5 mg/L) NAA(1.0～2.0 mg/L),最适分化培养基为MS 6-BA(0.3～1.0 mg/L) NAA(0.5～1.0mg/L);大叶补血草最适愈伤组织诱导培养基为MS 6-BA(0.3～0.5 mg/L) NAA 2.0 mg/L,最适分化培养基为MS 6-BA 1.0 mg/L NAA 0.5 mg/L.     

非洲菊组培快繁技术

以非洲菊幼嫩花托为外植体,采用MS作为基本培养基,添加不同生长激素对其进行组织培养研究。结果表明：非洲菊幼小花托MS＋BA3.0mg/L＋NAA0.2mg/L培养基上能很好地诱导形成愈伤组织并分化出不定芽,诱导率50%;继代培养基为：MS＋BA0.5mg/L＋NAA0.2mg/L,增殖系数2.8;生根培基为1/2MS＋NAA0.5mg/L,生根率可达90%。     

激素质量浓度及不同培养方式对马铃薯愈伤组织的诱导

以马铃薯幼茎为供试材料,通过在液体和固体的MS培养基中分别添加植物激素NAA和6-BA,进行愈伤组织诱导.实验结果表明,无论激素是添加在液体培养基还是固体培养基中,愈伤组织诱导率都随着NAA质量浓度的增加先升高后降低,液体培养基中愈伤组织最佳诱导率是77.78%（NAA2.5 mg/L＋6-BA0.5mg/L）;固体培养基中愈伤组织最佳诱导率是100.00%（NAA3.5mg/L＋6-BA0.5mg/L）.并且,固体培养基中添加激素的诱导率普遍高于液体培养基中添加激素的诱导率,表明液体培养基中添加激素的方式不能提高马铃薯的愈伤组织诱导.6-BA对马铃薯愈伤组织的诱导影响没有NAA显著.本文还对马铃薯未分化愈伤组织及分化出不定根的愈伤组织进行扫描电镜观察.     

香水草组织培养植株再生系统建立

香水草(Heliotropium arborescens)无菌实生苗下胚轴和子叶接种于含1.0mg/L 2,4-二氯苯氧乙酸(2,4-D)或2.0mg/L 6-苄氨酸嘌呤(BA)的MS培养基上能脱分化形成白色松散状或绿色致密状愈伤组织,但愈伤组织诱导率较低,分别为8.3%和26.5%.愈伤组织培养于含2.0mg/L NAA和0.5mg/L BA的MS培养上建立了具再生能力的愈伤组织继代培养方法.愈伤组织转移至含2.0mg/L BA和含0.1mg/L NAA的MS培养基上建立了不定芽分化再生方法.不定芽分化率最高91.3%.不定芽长大后,转移至含1.0mg/L IBA的减半MS培养基中不定芽生根率可达87.6%.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

The Analects and the Moment in Peking——The influence of a Confucian education system on the personality formation,the way of thinking and the fate of the main characters

0.Preface China has long been boasting of its colorful and immense culture which comprises of Taoism,Confucianism and Buddhism.The interwoven influence among the religions on Chinese way of thinking,on the nation＇s behavior in their social life throughout the long history is beyond list.