保肝口服液对CCl_4所致肝损伤小鼠护肝作用评价

为评价自研保肝口服液对急性肝脏损伤的保护作用,采用对受试小鼠进行分组及灌服给药,建立四氯化碳(CCl_4)急性肝脏损伤模型的方法,对肝脏进行病理学检查,观察受试动物肝脏病变情况;测定体内丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、丙二醛(MAD)及超氧化物歧化酶(SOD)的含量.结果显示空白对照组的肝脏细胞病理学结构正常且较清晰;模型组肝细胞变大、肿胀,大小不均;保肝口服液组和阳性组肝细胞颜色无明显变化,形态大致正常;生化指标显示口服液组的ALT,AST及MAD水平显著低于模型组,所测保肝口服液组的SOD含量水平显著高于模型对照组.结论:保肝口服液对CCl_4导致的急性肝损伤有一定的保护作用.     

中药解酒剂防止酒精性肝损伤的实验研究

通过小鼠解酒防醉药效、肝匀浆LPO值测定及其内脏器官观察和肝切片电镜观察的综合实验 ,考察了中药解酒剂F1、F2 和F3的解酒药效与延缓酒精中毒对肝功能损伤的功效之间的关系 .结果表明 ,解酒药效与保肝功能呈正相关 ;采用添加保肝中药的配方和中西药结合的方式 ,可增强解酒和保肝药效     

肝炎灵注射液抑制乙肝病毒有效时间观察

肝炎灵注射液是中药山豆根提取物。山豆根有抑制病毒、抗肝损伤、减轻肝细胞坏死、保肝降酶、促进肝细胞再生作用。此外，还有升高白细胞，提高血清白蛋白，降低球蛋白的作用。笔者采用肝炎灵注射液治疗慢性乙型肝炎患者32例，结果报道如下。     

清开灵指纹图谱验证方法学研究

清开灵注射液是从古方安宫牛黄丸拆方改制而成的一种中药注射剂,由七味药组成,是国家中药保护品种,具有保护肝脏、促进损伤肝细胞的修复、清热解毒、镇静安神等作用.本文对清开灵注射液的液相指纹图谱方法进行了方法学验证研究.     

中药混合液对麻黄素致孕鼠肝损伤及抗氧化功能的影响

为探讨中药混合液对麻黄素致损伤孕鼠肝组织的保护作用,将72例受孕小鼠随机分为对照组、麻黄素组和中药组,麻黄素组和中药组连续腹腔注射0.043g·kg-1剂量的麻黄素溶液,中药组在注射麻黄素溶液1h后灌胃0.2mL中药混合液,记录孕鼠行为的变化,比色法测定肝组织T-AOC、GST活性及MDA含量,生物显微技术观察孕鼠肝组织结构的变化.结果显示麻黄素组孕鼠饮食、饮水量减少,跳动次数增多,肝组织T-AOC、GST活性显著降低,MDA含量明显升高,肝组织出现不同程度的损伤,肝血窦扩张,内皮细胞脱落,肝小叶界限紊乱,肝细胞空泡化;中药组孕鼠饮食、饮水量增多,跳动次数减少,肝组织T-AOC、GST活性显著升高,MDA含量显著降低,肝组织结构损伤明显减轻,肝小叶界限清晰,内皮细胞脱落减少,肝细胞结构基本正常.表明麻黄素抑制细胞的抗氧化酶活性,对肝组织结构有明显的损伤作用,而中药混合液可显著提高组织细胞的抗氧化能力,改善细胞活力,对麻黄素致损伤孕鼠肝组织有一定的保护作用.     

中药地黄中抗化学性肝损伤的活性成分研究

中药药效物质基础的研究是近年来中药研究的热点领域之一。以HL-7702肝细胞构建肝细胞膜色谱,并运用到地黄抗化学性肝损伤的活性成分研究。首次发现地黄中的麦角甾苷和吉奥诺苷B1能与肝细胞结合,进一步证实这两个化合物对H2O2诱导肝细胞损伤具有一定的保护作用,是地黄中抗化学性肝损伤的活性成分。     

中药致肝毒性相关机制研究

随着我国中医药事业的快速发展,中药在诸多疾病的应用治疗上成果斐然,但不可否认的是,治疗过程中不断出现的毒副作用也是不容忽视的.众所周知,肝脏是机体内负责能量合成和物质代谢的中心器官,特别容易受到药物的损害,成为药源性组织损伤的主要靶器官之一.近年来,其作用机制研究取得了较大的进展,主要包括中药对肝脏细胞的直接损伤、氧化损伤、代谢损伤等.通过查阅相关文献,总结了中药致肝毒性的作用机制及其研究的思路和方法,以期为临床上中药的应用以及其作为保健品的开发,提供可靠的借鉴及依据.     

促肝细胞生长素联合还原型谷胱甘肽治疗重度慢性乙型肝炎

目的 观察促肝细胞生长素联合还原型谷胱甘肽治疗重度慢性乙型肝炎的临床疗效.方法 选择60例重度慢性乙型肝炎随机分为对照组和治疗组各30例,比较治疗前后两组病人的临床症状和生化指标等方面的变化.对照组给予甘草酸二铵、香丹等抗炎保肝支持及防治并发症治疗;治疗组在此基础上加用促肝细胞生长素和还原型谷胱甘肽联合治疗.结果 治疗组疗效明显优于对照组,治疗组在临床症状和生化指标改善等方面与对照组比疗效差异有显著性意义(P<0.05).结论 两药联用有助于改善临床症状和生化指标,缩短重度慢性乙型肝炎的病程.防治疾病进展.     

保肝I号对D-氨基半乳糖和脂多糖致小鼠急性肝损伤的保护作用研究

采用D-氨基半乳糖(D-GalN)联合脂多糖(LPS)诱导的小鼠急性肝损伤模型对保肝I号(BGYH)的保肝降酶及其可能机制进行研究.将小鼠随机分为正常对照组,模型组,BGYH低、中、高剂量组,水飞蓟素组和联苯双酯组.每天给药1次,连续9 d,末次给药后除正常组均腹腔注射D-GalN/LPS建立急性肝损伤模型.结果显示高剂量BGYH可以显著降低小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)水平,同时,肝组织切片观察结果表明高剂量BGYH使小鼠肝细胞坏死程度有所减轻.在细胞因子方面,中或高剂量BGYH均可明显降低肿瘤坏死因子(TNF-α)及白细胞介素6(IL-6)等炎性细胞因子的表达,并同时升高抗炎因子白细胞介素(IL-10)的含量.此外,小鼠肝脏中一氧化氮(NO)、诱导性一氧化氮合酶(iNOS)水平在给予BGYH后也有下降的趋势.研究结果表明,BGYH对D-GalN和LPS联合诱导的小鼠急性肝损伤具有保护作用,可能是通过抑制免疫因子的释放,调节免疫反应而实现.     

酒精对肝细胞损伤的自由基机理研究

为探讨酒精对肝细胞损伤的自由基机理,首先研究酒精对体外培养的L-02肝细胞的增殖和生化指标的影响.实验表明,酒精浓度在100mL·L-1以上时,共培养结束后30h内细胞存活率始终低于50%;当酒精浓度控制在80mL·L-1以下时,细胞恢复过程中存在存活率突降又上升的时间段;当酒精与细胞共培养时间为2、3、4h时,存活率突降又上升的时间段分别为18、12、6h.结果表明,酒精对肝细胞的损伤因浓度和与细胞共培养时间不同而造成化学损伤(即由酒精及其代谢产物本身的毒性直接杀死细胞)和自由基损伤.酒精引起的自由基损伤具有滞后性;随着与细胞共培养时间的延长,自由基攻击的时间会提前,并且所需的酒精浓度会降低.实验结果证实了酒精对肝细胞损伤确实与自由基有重要的关系,也为从清除自由基途径预防和治疗酒精性肝病提供一定的理论依据.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.     

Tracking data management using topology of moving object database system for moving object constrained trajectory in LBS application

Advances in wireless technologies and positioning technologies and spread of wireless devices, an interest in LBS (Location Based Service) is arising. To provide location based service, tracking data should have been stored in moving object database management system (called MODBMS) with proper policies and managed efficiently. So the methods which acquire the location information at regular time intervals then, store and manage have been studied. In this paper, we suggest tracking data management techniques using topology that is corresponding to the moving path of moving object. In our techniques, we update the MODBMS when moving object arrived at a street intersection or a curved road which is represented as the node in topology and predict the location at past and future with attribute of topology and linear function. In this technique, location data that are corresponding to the node in topology are stored, thus reduce the number of update and amount of data. Also in case predicting the location,because topology are used as well as existing location information, accuracy for prediction is increased than applying linear function or spline function.