文殊兰属植物化学成分提取分离及其生物活性

以近年来国内外对文殊兰属植物的研究报道为基础,归纳总结了该属植物的化学成分提取分离及生物活性的研究进展.文殊兰属植物含有多种化学成分,含有生物碱类、黄酮类、酚类等化合物,这些化合物具有抗肿瘤、镇痛、抗菌和抗病毒等多方面的生物活性.文殊兰属植物具有进一步研究开发价值.     

文殊兰种子中挥发油成分GC-MS分析

应用GC-MS分析文殊兰种子中的挥发油成分.采用水蒸气蒸馏法从文殊兰种子中提取挥发油,并用GC-MS对分离出的成分进行分析与鉴定,通过面积归一化法计算各成分的相对含量.从文殊兰种子中提取分离鉴定出挥发油成分37种.文殊兰种子中的挥发油成分主要是脂肪酸类化合物,占总提成分的55.05%,其中含量较高的成分是亚油酸(33.3%),邻苯二甲酸二丁酯(9.74%),邻苯二甲酸二异丁酯(8.99%).     

文殊兰弱碱性生物碱的抗肿瘤活性研究

研究文殊兰中弱碱性生物碱对不同肿瘤细胞的抑制作用,并筛选出敏感细胞株,观察凋亡形态;MCF-7细胞株、SMMC7721细胞株、SGC7901细胞株、HepG-2细胞株,MTT溶液,DMSO,Hoechst33258等;利用MTT法检测文殊兰弱碱性生物碱对上述肿瘤细胞的抑制作用以及Hoechst 33258染色法观察细胞凋亡的形态学变化;文殊兰弱碱性生物碱对上述细胞株均有明显的抑制作用,对HepG-2细胞的抑制作用更为明显,给药72 h后测定IC50=9.374 323μg/mL;Hoechst 33258染色法观察细胞出现凋亡小体,有凋亡现象发生.文殊兰弱碱性生物碱MCF-7细胞株、SMMC7721细胞株、SGC7901细胞株、HepG-2细胞株具有不同程度的抑制作用,且呈现一定的剂量依赖性,对HepG-2细胞的抑制作用最强,表现出良好的抗肿瘤活性.     

石蒜科6属6种植物叶表皮的初步比较研究

对石蒜科(Amaryllidaceae)中石蒜属(Lycoris Herb)，朱顶红属(Hippeastrum Herb)，文殊兰属(Crinum，L)，龙舌兰属(Agare L)，网球花属(Haemanthus L)，和君子兰属(Clivia Lindl)6属共6种植物的叶表皮结构在光学显微镜下进行了观察与比较研究。结果表明：石蒜科表皮细胞形状多数为长矩形，椭圆形或近圆形，无副卫细胞，但表皮细胞形状，大小以及气孔器分布密度在种属间存在一定的差异，可以作为鉴定该植物种及属的性状之一，同时也为石蒜科植物的系统演化提供了实验依据。     

广西柳州市主城区主要绿化植物叶片的全硫氮含量特征

通过测试,找出柳州市主城区吸收SO_2、NO_2能力强的植物,为今后城市生态修复和功能型园林绿化提供素材。以广西柳州市主城区内61种绿化植物为对象,采用HNO_3-HClO_4、H_2SO_4-H_2O_2消煮法,对其叶片的全硫、全氮含量进行测定。结果表明,所选61种绿化植物的全硫含量为0.024%-0.711%,全氮含量为0.007%-0.207%。根据硫含量,可将61种绿化植物分为3个等级,其中一级(≥0.55%)有1个种,二级(0.15%-0.55%) 26个种,三级(≤0.15%) 34个种。根据全氮含量,可将61种绿化植物分为3个等级,其中一级(≥0.14%)有10个种,二级(0.06%-0.14%) 38个种,三级(≤0.06%) 13个种。61种绿化植物全硫、全氮含量均较高的为文殊兰、洋紫荆、秋枫、合果芋、鸢尾、荷花玉兰、朱槿、红花羊蹄甲、木槿、针葵、冷水花、红叶李、鸡冠刺桐、吊钟、吊竹梅、紫色梅、山杜英、银海枣、毛杜鹃,这些植物吸收SO_2、NO_2能力强,在治理城市空气污染方面具有较好的应用前景。     

黄精属植物的研究进展

黄精属植物是具有中药价值的多年生草本植物。本文对黄精属植物的研究现状,包括形态分类、染色体核型、孢粉及开发利用等进行了概括,并对黄精属植物的未来研究方向进行了展望,为黄精属植物的进一步研究提供了基础资料。     

植物抗寒机理研究进展

低温寒害是影响农作物分布、产量和品质的主要胁迫因子.植物抗寒机理研究是比较活跃和进展很快的领域.从植物抗寒的生理生化基础、植物对低温的信号感知、低温信号在植物中的传递及植物冷反应基因的表达调控等四个方面对目前植物抗寒机理的相关研究进行了综述,并对植物抗寒研究在农业生产中的应用进行了展望.     

我国木兰科植物组培快繁研究进展

简述了木兰科植物组织培养在种苗繁育上的意义,对近年来我国木兰科植物组培快繁技术研究现状进行了详细概述,包括木兰科植物无菌培养体系建立、增殖培养、生根培养及移栽炼苗,分析总结了木兰科植物在植物组织培养过程中存在的关键问题,并对木兰科植物组织培养的发展前景进行了研究展望。     

反转录转座子分子标记及其在植物研究中的应用

反转录转座子以高拷贝在植物基因组中广泛分布,具有高度的异质性,可作为分子标记对植物基因组进行研究.文章对SSAP、IRAP、REMAP及RBIP4种基于植物反转录转座子开发的分子标记技术的原理、技术流程、特点及其在植物研究中的应用进行了概述.     

广西雅长保护区兰科植物保育研究进展

为了解广西雅长兰科植物国家级自然保护区(以下简称雅长保护区)兰科植物保育研究概况,通过文献检索统计分析并结合实际,对雅长保护区在兰科植物资源特征、兰科植物繁育、传粉生物学等研究进行梳理总结,提出兰科植物研究科学发展建议,为今后保护区兰科植物保育研究提供参考方向及借鉴资料。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.