| 文殊兰弱碱性生物碱的抗肿瘤活性研究 |
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| 引用本文： | 吴 迪,代岐昌,季宇彬.文殊兰弱碱性生物碱的抗肿瘤活性研究[J].哈尔滨商业大学学报(自然科学版),2014(2):150-153. |
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| 作者姓名： | 吴 迪 代岐昌 季宇彬 |
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| 作者单位： | [1] 哈尔滨商业大学生命科学和环境科学研究中心,哈尔滨150076 [2]国家教育部天然抗癌药物工程技术研究中心,哈尔滨150076 [3]哈尔滨商业大学药物研究所博士后科研流动站,哈尔滨150076 |
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| 基金项目： | 黑龙江省研究生创新科研项目（YJSCX2012-146HLJ）． |
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| 摘 要： | 研究文殊兰中弱碱性生物碱对不同肿瘤细胞的抑制作用,并筛选出敏感细胞株,观察凋亡形态;MCF-7细胞株、SMMC7721细胞株、SGC7901细胞株、HepG-2细胞株,MTT溶液,DMSO,Hoechst33258等;利用MTT法检测文殊兰弱碱性生物碱对上述肿瘤细胞的抑制作用以及Hoechst 33258染色法观察细胞凋亡的形态学变化;文殊兰弱碱性生物碱对上述细胞株均有明显的抑制作用,对HepG-2细胞的抑制作用更为明显,给药72 h后测定IC50=9.374 323μg/mL;Hoechst 33258染色法观察细胞出现凋亡小体,有凋亡现象发生.文殊兰弱碱性生物碱MCF-7细胞株、SMMC7721细胞株、SGC7901细胞株、HepG-2细胞株具有不同程度的抑制作用,且呈现一定的剂量依赖性,对HepG-2细胞的抑制作用最强,表现出良好的抗肿瘤活性.
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| 关 键 词： | 文殊兰 抗肿瘤 生物碱 MTT法 Hoechst 33258染色法 |
Research on anti-tumor activity of Alkalescent alkaloids from crinum |
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| WU Di,DAI Qi-chang,JI Yu-bin.Research on anti-tumor activity of Alkalescent alkaloids from crinum[J].Journal of Harbin University of Commerce :Natural Sciences Edition,2014(2):150-153. |
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| Authors: | WU Di DAI Qi-chang JI Yu-bin |
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| Institution: | 1. Center of Research on Life Science and Environmental Science, Harbin University of Commerce, Harbin 150076, China; 2. Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin University of Commerce, Harbin 150076, China ;3. Postdoctoral Programme,Institute of Material Medica of Harbin University of Commerce, Harbin 150076, China) |
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| Abstract: | Research the anti-tumor activity of Alkalescent alkaloids from Crinum .And screen the sensitive cells then observe morphological changes of cell apoptosis; MCF -7 cell, SMMC7721 cell, SGC7901 cell, HepG-2 cell, MTT solution, DMSO,Hoechst 33258;ex-periments could illustrate the use of the drug inhibition of these tumor cells by MTT assay .It could observe morphological changes of cell apoptosis by Hoechst 33258 staining; Alkales-cent alkaloids from crinum could inhibit these cells obviously .HepG-2 cell was inhibited more sensitive to alkaloids .After dosing 72 h it showed that IC 50 =9.374323μg/mL;it can be seen apoptotic bodies and apoptotic phenomenon by Hoechst 33258 staining;the results indicated that the Alkalescent alkaloids from crinum could inhibit the MCF -7, SMMC7721, SGC7901 , HepG-2 cells significantly .The suppression was in a dose dependent manne . The inhibition of HepG-2 was the strongest .It could show the excellent anti-tumor activity. |
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| Keywords: | crinum anti-tumor alkaloids MTT |
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