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1.
鸡Zong菌菌丝体液体培养的研究   总被引:1,自引:0,他引:1  
适合鸡Zong菌菌丝液体培养的培养基成分是:淀粉10g,蔗糖2g,蛋白胨2g,KH2PO10.3g,MgSO4.7H2O0.15g;最佳C/N为30-40:1;最适培养温度26C;pH6.4;振荡频率140-150r/min。继代培养周期8天,液体培养的鸡Zong菌菌线体氧基酸总可达273mg/g干重,其中42.41%为人体必需的氨基酸(未含色氨酸)。  相似文献   

2.
以食用菌白木耳为试验菌种,通过斜面、摇瓶培养及深层发酵正交实验,研究了各培养阶段的基质组成。其结果得出,种子摇瓶培养基(g/100ml):马铃薯20%、葡萄糖2%、蛋白胨0.2%、KH_2PO_40.15%、MnSO_40.01%、CMC-Na1%。深层发酵培养基组成为:土豆汁1.5%、玉米粉2.0%、麸皮3%、KH_2PO_40.2%、吐温-80为0.1%,其发酵产物菌丝体中所含粗多糖达13.23%,蛋白质含量为32.83%、氨基酸为19.75mg/100g干菌葡萄体,必需氨基酸为7.67mg/100g干菌丝体。  相似文献   

3.
采用振荡培养方法,研究了松密环菌ns1的生理生化特性,结果表明,松密环菌以葡萄糖为碳源,(NH4)2SO4和(NH4)H2PO4为氮源,C、N质量比为10:1和16:1以及PH5-5.5,培养摇床转速80-10r/min,矿物质和生长因子的需要,在25-27℃培养下96h时最佳,其菌丝体产量最高, 为2-325g/L。  相似文献   

4.
以三因素三水平的正交试验设计培养条件,接种量5%(V/v),pH=6.5,120r/min,18℃,72h,对虫草菌进行液体培养.通过对生长的菌丝体干重的测定及分析,得到该菌种生长最适培养基配方为:葡萄糖,20g/1000ml;NH4NO3,1g/1000ml;K2HPO4,1g/1000ml.并证明碳源为其生长的最重要营养因素  相似文献   

5.
不同电位下催化电合成聚吡咯膜的电化学行为   总被引:1,自引:0,他引:1  
通过循环伏安法研究了以T_sOH(对甲苯磺酸),HCl_4,H_2SO_4,NaClO_4和Na_2SO_4为支持电解质,Tiron为催化剂,在不同电位范围催化电合成聚吡咯(PPE/Pt)和该膜电极在相应支持电解质水溶液中的电化学行为.发现PPE/Pt的CV图、稳定性和电活性随聚合电位及阴离子种类的变化明显不同于非催化电合成聚吡咯.在0~0.8V电位范围制得的PPE/Pt的稳定性最好,并随支持电解质的变化按T_sOH,HClO_4,H_2SO_4,NaClO_4和Na_2SO_4的顺序减小.同时讨论了产生变化的原因.  相似文献   

6.
将28只摘除卵巢后6~7周的山羊随机分为4组,按2×2因子实验设计进行处理,实验因子为:①肌肉注射孕酮(P_4):1~5天每天10mg;6~12天每天20mg.②肌肉注射雌二醇(E_2):第13天20ug;第14天40ug.对照处理则注射相应体积的玉米油,在第1~15天每天采1次颈静脉血样,用RIA法测定血浆P_4和E_2水平,结果表明,每天注射10和20mgP_4可使血浆P_4水平分别维持在3和5ng/ml左右,注射20和40μgE_2可分别产生30和50pg/ml的血浆E_2水平。用这种方法,简便而准确地模拟了山羊发情周期中血浆P_4和E_2水平的变化范型。  相似文献   

7.
报道了高价钨砷杂多酸盐[(C_4H_9)_4N]_21;[KAs_4W_40O_140(CrO)_2]、[(C_4H_9)_4N]_21[KAs_4W_40O_140Co_2]和[(C_4H_9)_4N]_21[KAs_4W_40O_140Mn_2]的合成,表征及其氧化性质.通过元素分析、磁化率、电子吸收光谱及e.s.r谱证明,标题化合物中取代元素Cr、Mn、Co的氧化态分别为5,3,3.合成化合物具有氧化性质,在甲苯中能将三苯基磷、苯乙烯及反-二苯乙烯氧化成氧化物.  相似文献   

8.
虫草菌最适培养基的正交试验研究   总被引:4,自引:0,他引:4  
以三因素三水平的正交试验设计培养,接种量5%(V/v),pH=6.5,120r/min,18℃,72h,对虫草菌进行液体培养,通过对生长的菌丝体干重的测定及分析,得到该菌种生长最适培养基配方,葡萄糖,20g/1000ml,NH4NO3,1g/1000ml,K2HPO4,1g/1000ml并证明碳源为其生长的最重要营养因素。  相似文献   

9.
拟定了测定微量锌(Ⅱ)的新方法,研究了锌(Ⅱ).水杨基荧光酮-阳离子表面活性剂体系的显色反应,实验证明,在pH(9.0~9.7)的H_3BO_3-H_3PO_4-HAc-NaOH缓冲体系中,锌(Ⅱ)与水杨基荧光酮生成1:2的配合物,在阳离子表面活性剂-溴化十六烷基三甲胺(CTMAB)存在下生成1:2:3的蓝紫色三元配合物,λ_(max)为570nm,表观摩尔吸光系数ε为7.7×10 ̄4L·mol-1·cm-1,可在水相中直接测定锌(Ⅱ);并且详细研究了最佳实验条件,锌(Ⅱ)含量在(1.0~8.0)μg/25mL,范围符合比耳定律,锌(Ⅱ)的检测限为0.04μg/mL,用于快速测定食品中的微量锌(Ⅱ),结果满意。  相似文献   

10.
探讨了1-硝基蒽醌(1-NO_2AQ)和1-氨基蒽醌(1-NH_2AQ)同时测定和分别测定的色谱条件,提出了在Shim-packCLSS-SIL色谱柱上,以正庚烷-1,4二氧六环-乙酸乙酯(65:35:5(V/V)为流动相,流量为1.5ml/min,柱温40℃,以分光光度检测器于254nm处检测时,4min内使1-NO_2AQ与1-NH_2AQ分离;5min使1-NH_2AQ与杂质分离;7min可使1-NO_2AQ与杂质分离的高效液相色谱分析法。其绝对检出下限1-NO_2AQ为0.012μg,1-NH_2AQ为0.026μg,变异系数分别为1.1%和0.96%,回收率分别为99.7%和100.2%。  相似文献   

11.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

12.
AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…  相似文献   

13.
本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。  相似文献   

14.
Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with  相似文献   

15.
The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to  相似文献   

16.
17.
The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.  相似文献   

18.
R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).  相似文献   

19.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

20.
The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.  相似文献   

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