HPLC测定冷浸法提取中成药中的甘草酸含量

报道了以 PC8为色谱柱 ,甲醇 :水 :醋酸 (5 7.0∶ 42 .6∶ 0 .4,V∶ V∶ V)为流动相 ,在 UV2 5 4nm为测定波长的条件下 ,利用外标法定量 ,建立了反相高效液相色谱法测定冷浸法提取自制的 (依据经典成方 ) 8种方剂和 12种市售中成药中甘草酸含量。实验表明 ,本法操作简便、快速、结果准确 ,适用于测定不同类型制剂中甘草酸的含量。     

高效液相色谱法测定安脑牛黄胶囊中栀子苷含量

目的　建立安脑牛黄胶囊中栀子苷含量测定方法 .方法　采用C1 8柱 ,以乙腈 -水 (1 0∶90 )为流动相 ,检测波长为 2 38nm .结果　线性范围为 0 .1 6～ 0 .80 μg ,回收率为 99.2 5 % ,RSD为 1 .30 % .结论　该方法简便易行 ,可供安脑牛黄胶囊中栀子苷质量控制     

高效液相色谱法检测红发夫酵母胞内虾青素

确定了红发夫酵母的破壁提取方法并采用高效液相色谱法定量地测定了红发夫酵母胞内虾青素的含量。试验得到的液相色谱条件为:色谱柱KromasilC18, 5μm, 250mm×4. 6mm;流动相为甲醇和乙腈,V甲醇∶V乙腈=9∶1;流速1. 4mL/min;检测波长478nm;柱温为室温。加标回收率为(98. 2~102. 8)00。     

HPLC法测定双黄连片中绿原酸的质量分数

探讨高效液相色谱法测定双黄连片中绿原酸含量的方法 ,控制其质量 .Shim -packVP -ODS(4 6mm× 15 0mm ,5 μm)色谱柱 ,流动相为甲醇 -水 -冰醋酸 (2 0∶80∶1) ,流速为 1 0mL/min ,检测波长为 32 7nm .绿原酸在 0 4 0 8～ 2 0 4 0 μg范围内呈良好的线性关系 ,回归方程为A =2 84 0 95 98C +6 894(r=1.0 0 0 ) ,平均回收率为 10 0 .98% (RSD为 1.13% ) ,重复性RSD为 0 .32 % .方法简便、快速、灵敏、准确、重现性好 ,可作为双黄连片中绿原酸的质量控制标准     

槲皮素的阳极伏安行为研究

研究了槲皮素在玻碳电极上的阳极伏安行为 .在pH =5 .8的Na2 B4O7(0 .0 5mol L) -NaH2 PO4(0 .1mol L)缓冲溶液中 ,槲皮素于 +0 .2 0V(vs .SCE)左右产生一个灵敏的阳极氧化峰 ,其浓度与峰电流在 8.0× 10 - 8- 1.0× 10 - 4 mol L范围内呈良好线性关系 ,检测限为 2 .6× 10 - 8mol L .用本法不需分离直接测定了模拟血清中的槲皮素 ,相对标准偏差为 2 .1%,回收率为 98.5 %.     

反相高效液相色谱法测定三白草中槲皮素和芦丁的含量

采用反相高效液相色谱法 (RP -HPLC)在C1 8色谱柱上以甲醇 :0 .2 %磷酸溶液 (5 0∶5 0 )为流动相测定了三白草中槲皮素和芦丁的含量 .流动相的流速为 1mL min ,检测波长 370nm .结果表明槲皮素在 0 .0 0 8-0 0 84 μg、芦丁在 0 .0 5 4 - 0 .5 4 0 μg范围内呈良好的线性关系 ,相关系数分别为 0 .9988和 0 .9999.槲皮素回收率为 99% ,RSD为 2 .0 % (n =5 ) ,芦丁为 10 6 % ,RSD为 1.4 % (n =5 ) .     

高效液相色谱法测定柯柯豆碱母液中柯柯豆碱、咖啡因的含量

控制提取工艺需要测定柯柯豆碱母液中柯柯豆碱、咖啡因的含量。采用高效液相色谱法测定柯柯豆碱母液中柯柯豆碱、咖啡因的含量 ,以 C8柱为色谱柱 ,以 V(体积分数为 0 .0 0 4 5 %的高氯酸 )∶ V(乙腈 ) =80∶ 2 0为流动相 ,检测波长为 2 80 nm,柯柯豆碱与咖啡因色谱峰的分离度大于 4 ,线性范围为 10～ 80μg/ m L ,相关系数大于0 .999,回收率大于 99.0 %。     

HPLC法测定蒙药地格达—4味汤中栀子苷的含量

运用HPLC建立蒙药“地格达— 4味汤”中栀子苷含量的方法 .采用C18柱 ,乙腈一水 (15∶85 )为流动相 ,流速 1 0ml/min ,检测波长 2 38nm .结果该法平均回收率 99 1% ,RSD =1 78% (n =5 ) .该方法简便 ,快速 ,准确 ,为测定蒙药地格达— 4味汤中栀子苷含量提供了可靠的方法     

高效液相色谱法测定脑正清冲剂中芍药苷的含量

建立测定脑正清冲剂中芍药苷含量的高效液相色谱法 .方法 :KromasilC1 8色谱柱 (2 5 0mm× 4 6mm 5 μm) ,以甲醇 水 (2 5∶75 )为流动相 ,流速为 1 0mL min ,检测波长为 2 30nm .柱温 :室温 .结果 :芍药苷在 0 5 0 5～ 4 0 0 0 μg范围内峰面积与其浓度线性关系良好 (r =0 9992 ) ,平均回收率为 98 36 % ,RSD为 1 4 8% (n =5 ) .结论 :该方法准确、简便、快速 ,灵敏度高 ,重现性好     

反相液相色谱法测定维生素D_2微粒胶囊

采用反相高效液相色谱法测定维生素D2 微粒胶囊中VD2 的含量。用AlltechC18柱 ( 1 5 0mm× 4 6mmID ,3μm) ,流速为 1 0mL·min- 1,流动相为V(甲醇 )∶V(水 ) =90∶1 0 ,以苯并 [e]芘为内标 ,UV2 6 5nm检测 ,本法简便、准确、重现性好[1] [2 ] 。     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

Perspherical Approaches for Electrons in Atoms and Charged Excitons in Quantum Wells

Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to     

Advances in broadband wireless communications under high-mobility scenarios

Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications （up to 574.8 km/ h test speeds or 380 km/h commercial speeds） in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.     

Wuhan University Journal of Natural Sciences TOTAL CONTENTS

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海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Scale-based spatial data model for GIS

Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to