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1.
对在实验室培养的含有人工感染龙血居吸虫尾蚴发育各期的折叠萝卜螺进行详细的组织学和组织化学观察、龙江血居吸收尾蚴发育过程中大至可分为以5期:胚细胞期、胚球期、尾蚴胚体期、成熟尾蚴前期,成熟尾蚴期,成熟尾蚴体内的腺体有3种类型;紧接在头器缢缩之后的一对腺体分泌富含酸性粘多糖类物质;体中背部3对较大腺体分泌富含粘蛋白类物质;体中部另外有5对腺体分泌富含糖原类物质,此外,对实验室培养的含龙江血居吸虫成虫和虫卵的金鱼组织器官,含侵入0.5-2h龙头血居吸虫童虫的鲫鱼苗也进行了组织和组织化学观察。  相似文献   

2.
运用组织学、组织化学等方法对食蛏泄肠吸虫(Vesicocoeliumsolenophagum)尾蚴在缢蛏(Sinonovaculaconstricta)体内不同发育阶段进行了观察.食蛏泄肠吸虫尾蚴发育主要分为:胚细胞期、胚团期、尾蚴雏体期、尾蚴发育早期、尾蚴发育中期和成熟尾蚴.在尾蚴的发育过程中,组化物质变化明显:发育越成熟尾蚴体被糖原类物质的含量越丰富,体被下含酸性多糖类物质细胞的数量越多  相似文献   

3.
观察了诺丹冰草胚和胚乳的发育过程.一般合子第一次分裂为横分裂,但偶见有斜分裂,形成顶细胞和基细胞.顶细胞纵分裂,基细胞横分裂,胚胎发生属紫宛型.一个助细胞于受精前解体,宿存助细胞生存到椭圆形胚期.丝状器具强的PAS正反应.反足细胞于受精后增殖并保留到多细胞原胚时消失且无多糖反应.核型胚乳,胚乳细胞化开始于多细胞原胚期.成熟胚乳细胞中具许多大的淀粉粒.种子有胚乳.发现有原胚的移位及胚囊中缺少胚的现象.  相似文献   

4.
扬子鳄胚胎胃PAS反应观察   总被引:2,自引:0,他引:2  
选取23例扬子鳄胚胎,对其胃进行PAS反应观察.胃表面上皮:第30天时,上皮细胞表面出现薄层的PAS反应阳性物质;第34~60天,阳性反应逐渐增强,并出现粘液保护层.胃腺:胃底腺,第40天腺芽上皮出现PAS反应阴性细胞,第46~60天胃底腺腺管基部上皮细胞呈阴性反应;幽门腺及贲门腺,第40天时出现腺芽,腺芽细胞呈阳性反应;第46~60天幽门腺及贲门腺腺管基部出现阴性反应细胞.  相似文献   

5.
番木瓜体细胞胚发生及发育的影响因素   总被引:8,自引:0,他引:8  
研究了影响番木瓜(Caricapapaya)体细胞胚发生、发育的一些因素.结果表明:①番木瓜无菌小苗幼根在附加NAA1.0mg/L+KT0.5mg/L+GA30.5mg/L的改良MS培养基上可诱导出大量具良好胚性潜能的愈伤组织;②愈伤组织在附加NAA0.25mg/L+KT0.25mg/L的改良MS培养基上可连续继代并得到胚性化;③在所试验的激素种类中,KT是适于体细胞胚的发育,最适浓度为0.5mg/L;④0.1%~0.3%的活性炭有利于体胚的发生、发育;⑤体细胞胚在固体培养基上完成球形胚期前的发育,在液体培养液中完成球形胚期后的发育可提高成熟体胚的产量和质量  相似文献   

6.
将花生实生苗的幼叶接种于MS固体诱导培养基上诱导体细胞胚发生。用PAS染色法观察了体细胞胚发生发育过程中的淀粉代谢的动态变化。结果表明,在体细胞胚的发育过程中,淀粉表现出两次积累高峰,第一次在胚性细胞团中,淀粉粒大而密集;随着胚性细胞的分裂,淀粉逐渐被消耗,到多细胞原胚期胚体内淀粉趋于消失。球形胚期,淀粉粒又开始积累,出现第二次积累高峰;到成熟胚期,淀粉积累只限于生长点区域,淀粉的两次积累高峰为体  相似文献   

7.
对厦门地区龙海麻鸭自然感染菲莱氏盲肠温扬球虫亚种的季节动态进行了调查,结果表明一年四季均可感染,其中流行高峰期是3~7月.对盲肠组织进行石蜡切片的细胞化学染色,显明:成熟的裂殖体含有多糖,成熟大配子体、小配子体和卵囊PAS反应呈阳性,而滋养体、早期的大小配子体PAS反应呈阴性  相似文献   

8.
H.soongolicum叶片外植体在MS加IAA10mg/L,6-BA1mg/L培养基上获得愈伤组织,然后在MS加IAA5mg/L,6-BA1mg/L培养基上获得胚状体及试管苗.愈伤组织的诱导及其再分化对IAA有特殊的要求,2.4-D不能取代IAA的作用;胚状体来源于单个胚性细胞,由它经均等或不均等分裂形成的细胞后代继续发育为各级胚;试管苗根系发育不良。作者认为该种的濒危与其胚胎发生关系不大,而胚根的不正常发育可能是导致其濒危的原因之一。  相似文献   

9.
采用SDS-PAGE、电泳转印技术和固相免疫标记技术结合的酶联免疫印渍技术(ETIB),首次分析东毕血吸虫成虫抗原。抗原蛋白组分显示:SD-PAGE共将绵羊东毕血吸虫成虫抗原分离出42条带;经ETIB分析,与阳性血清反应有12条带,其中主带有4条,分子量分别为35、80、92和94kD,试验表明,这四个分子量的蛋白质具有较强的反应原性,可做为东毕血吸虫的诊断抗原。  相似文献   

10.
Carnoy固定,常规石蜡包埋、切片、H.E染色、PAS反应、RNA反应,对无蹼壁虎消化道的组织学进行光镜观察.结果表明:其消化道由同心圆的粘膜层、粘膜下层、肌层和浆膜层组成.绒毛发达,绒毛的上皮细胞为单层柱状上皮.消化腺为分枝管状腺.PAS反应显示柱状细胞纹状缘,杯状细胞和腺细胞呈强阳性,与分泌的粘多糖有关.RNA反应显示腺细胞呈强阳性,与腺细胞分泌活动有关.  相似文献   

11.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

12.
AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…  相似文献   

13.
本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。  相似文献   

14.
Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with  相似文献   

15.
The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to  相似文献   

16.
17.
The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.  相似文献   

18.
R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).  相似文献   

19.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

20.
The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.  相似文献   

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