金钗石斛花芽蛋白质提取及其双向电泳体系优化

通过对蛋白质提取方法、水化方式、等点聚焦程序、IPG胶条选择等方面的优化，建立金钗石斛花芽蛋白质双向电泳体系.结果表明，采用酚抽提法提取蛋白，样品与水化液1∶〖KG-*2/3〗40复溶；被动水化，等点聚焦程序B2；选用24 cm pH 3～10NL 胶条和12% 的凝胶；采用考马斯亮蓝G-250染色，获得了可识别1 422个蛋白质斑点的双向电泳图谱，且重复性好、分辨率高，为进一步开展金钗石斛花芽在蛋白质组学水平上的分析提供了技术支持.     

人表皮组织蛋白质组双向电泳技术的建立

目的:建立和优化人表皮组织蛋白质组双向电泳技术,为后续的皮肤蛋白质组学研究奠定基础.方法:组织匀浆法制备表皮组织总蛋白,采用固相pH 梯度胶条进行双向电泳,凝胶银染后用PDQuest 7.4软件对电泳图谱进行分析.结果:人表皮组织蛋白质组在7cm pH 5～8 IPG胶条的最佳上样量为200 靏,平均蛋白斑点数为414±19,平均匹配斑点数为346±31,匹配率达83.6%.结论:通过优化双向电泳条件,获得了分辨率高、重复性好的人表皮组织蛋白质组双向电泳图谱.     

盐芥根蛋白双向电泳体系的建立

为建立根蛋白质组双向电泳体系,对植物种植条件、蛋白提取方法I、PG干胶条的长度和pH范围、等电聚焦及SDS-PAGE参数进行探索和优化,建立了适用于盐芥(Thellungiella halophila)根蛋白质组学研究的双向凝胶电泳技术体系.     

肝癌细胞株HepG-2的G1期细胞蛋白质组双向电泳图谱的建立

采用双向电泳分离肝癌细胞株 HepG2 的 G1 期细胞的蛋白质,经考马斯亮蓝染色及图像扫描与分析显示:在 pH3-10,13 cm 的 IPG 胶条上,可分离到 227 个重复性及分辨率均较好的蛋白斑点,蛋白斑点的匹配率为 84%.建立了 HepG2 的G1 期细胞蛋白质组双向电泳图谱,为其蛋白质组学的进一步研究奠定了基础.     

华溪蟹主要组织蛋白质双向电泳技术体系的建立

为深入研究重金属胁迫下华溪蟹主要组织蛋白质的差异表达,对华溪蟹心脏及鳃总蛋白质的双向电泳技术体系进行了初步探讨.通过对样品处理方法、上样量大小以及双向电泳实验条件的比较选择,初步建立了适用于华溪蟹组织蛋白质组分析的双向电泳技术体系.结果表明:选取pH 3～10的24cmIPG胶条,上样量1.0mg,得到了较为理想的华溪蟹心脏及鳃双向电泳图谱;分离到的心脏蛋白质多集中于pH 5.5～9,而鳃蛋白质多集中于pH 4～7.     

适用于小麦叶片总蛋白分析的双向电泳技术

双向电泳技术越来越成为植物蛋白质组研究中的关键技术,样品制备、固相预制胶条水化、等电聚焦及SDS—PAGE都是影响双向电泳结果的重要步骤,本研究以晋麦47号小麦叶片为研究材料,在样品除盐、预制胶条水化方式、第二向SDS—PAGE电压选择方面进行了探索与优化,通过比较双向电泳图谱,建立适用于小麦叶片总蛋白质分析的双向电泳技术体系．结果表明：样品经多步除盐并加长除盐时间后进行双向电泳,蛋白能较好地被分离,2-DE图谱蛋白点较多,分辨率较高;IPG胶条先后经被动水化与主动水化比仅主动水化的2-DE图谱蛋白点损失少;第二向SDS—PAGE中电压选择120V较200V的2．DE图谱蛋白点拖尾少,纹理现象少．     

橡胶草叶片蛋白质提取方法的选择及双向电泳体系的优化

目的为了建立起橡胶草叶片组织的蛋白双向电泳体系。方法采用5种不同方法提取橡胶草叶片蛋白,并通过蛋白提取率和单向SDS-PAGE电泳的比较,筛选出改良TCA-丙酮沉淀法、酚-乙酸铵沉淀法、Tris浸提法3种方法进行2-DE电泳。结果表明选择改良TCA-丙酮法为最合适的蛋白质提取方法。为进一步优化体系,比较了不同染色方法、上样量和固相p H梯度预制胶条(IPG)的p H范围对双向电泳结果的影响,电泳结果表明使用17 cm p H 3-10的IPG胶条、上样量为1200μg、考马斯亮蓝染色法能分离得到更多的蛋白点,是橡胶草叶片双向电泳体系的最优条件。结论本研究初步建立了橡胶草叶片蛋白质双向电泳体系,为深入研究橡胶草蛋白质组学提供依据。     

蚯蚓蛋白质组学研究中3种总蛋白提取方法的比较

双向电泳(2-DE)技术在现阶段的蛋白质组学研究当中,由于其价格相对便宜,仪器设备相对简单而被广泛应用.合适的总蛋白质样品制备方法,可以提高双向电泳蛋白点的数目和分辨率以保证蛋白质组信息的完整性,是整个实验的关键.蚯蚓作为土壤污染和土壤生态毒理学研究的重要生物,其总蛋白质提取的方法学研究还不完善.通过对已发表的文献中的蛋白提取方法进行筛选和优化,利用SDS-PAGE电泳和双向电泳的结果,对三氯乙酸-丙酮沉淀法、裂解液法和酚抽提法对蚯蚓总蛋白提取结果进行了评价,建立了适合蚯蚓蛋白质组学研究的总蛋白样品制备方法.     

油菜双向电泳技术体系的优化

为建立一套可以较好地分离油菜不同器官(花蕾、化药、叶片)的双向电泳技术体系,对IPG胶条的pH范围、凝胶染色的方法以及蛋白质的上样量进行优化.结果表明,以pH=4~7,17cm的IPG胶条进行双向电泳,胶体考马斯亮蓝染色,蛋白质上样量为800μg时,油菜的花蕾、化药以及叶片蛋白质均可得到很好的分离,获得的2-DE凝胶图谱清晰.为检验优化后的双向电泳技术体系与质谱的兼容性,选取10个蛋白质点进行肽质量指纹图谱分析,发现这些蛋白质点经质谱分析后均可得到成功鉴定.表明该分离体系与质谱兼容性较好,蛋白质点鉴定成功率较高.     

大型海藻龙须菜蛋白提取及双向电泳体系的优化

本文以龙须菜为材料,比较了尿素/硫脲法,改良丙酮沉淀法,Trizol法3种不同蛋白质提取方法;并从胶条长度及pH范围,上样量,分离胶浓度等因素探讨了双向电泳技术体系的优化.结果表明:采用Trizol法辅以超声破碎提取龙须菜总蛋白效果优于尿素/硫脲法和改良丙酮沉淀法;同时,采用pH 4-7,11 cm胶条,上样量为800μg,分离胶浓度为12%的双向电泳条件可获得背景清晰,分辨率好的二维电泳图谱.该体系也可以为龙须菜属和江蓠属其他海藻的蛋白质双向电泳分析提供参考.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.