反相高效液相色谱法测定烟叶中茄尼醇的含量

采用反相高效液相色谱法研究了测定烟叶提取物中茄尼醇含量的方法.条件为:Hypersil ODS柱,流动相为甲醇∶乙醇=75∶25(v/v),流速1.0 mL/m in,紫外检测波长211 nm,柱温30℃.茄尼醇的质量浓度为2.16~34.56 mg/L时,峰面积与质量浓度具有良好的线性关系,相关系数为0.99998.方法的检出限(S/N≥3)为0.09 mg/L,相对标准偏差(RSD)为0.42%(n=6),平均回收率为99.2%.该方法快速、简便、准确.     

RP-HPLC法分析罗红霉素含量

目的 建立用RP-HPLC法分析罗红霉素药物含量的方法.方法 以安捷伦ZORBAX Eclipse XDB-C18柱为色谱柱、乙腈(0.010mol/L)醋酸铵缓冲液(体积比70:30,Ph=6.5)为流动相,选择220nm为检测波长;柱温40℃,用反相高效液相色谱法测定罗红霉素(Roxithromycin,RM)的含量.结果 罗红霉素的浓度在1.150-100.8 mg/L范围内与峰面积呈良好线性关系,r=0.99977,平均回收率为99.57%,相对标准偏差为1.23%(n=5).结论 该方法操作简单、快速,可用于罗红霉素含量的测定.     

HPLC法测定恩杂鲁胺原料药的含量

为建立HPLC法测定恩杂鲁胺原料药的含量,采用Ultimate LP-C18(250×4.6 mm,5μm)色谱柱,以甲醇-0.1%磷酸二氢钾溶液(70∶30)为流动相,流速为1.0 m L/min,进样量为10μL.结果表明,恩杂鲁胺的浓度在12 52μg/m L范围内与峰面积呈现良好的线性关系(r=0.999 2),平均回收率为99.18%100.10%(n=3),RSD为0.4%0.5%.本法具有分析速度快、简单、准确以及专属性强好等特点,适合恩杂鲁胺含量的测定.     

HPLC-ELSD法测定清开灵滴丸中胆酸和猪去氧胆酸的含量

建立了高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定清开灵滴丸中胆酸和猪去氧胆酸含量的方法.采用汉邦C18色谱柱(200 mm×4.6 mm,5μm),流动相为0.1%甲酸-甲醇-乙腈溶液(25∶60∶15),柱温30℃,漂移管温度为105℃,空气流速2.2 L/min.胆酸和猪去氧胆酸的进样浓度分别在0.598¹.596 mg/mL(r=0.9992)和0.6001.596 mg/mL(r=0.9992)和0.600⁰.1.600 mg/mL(r=0.9995)范围内其线性关系良好,胆酸和猪去氧胆酸平均回收率分别为99.74%和99.21%.该方法准确,简便,分离度高,重现性好,可用于清开灵滴丸中胆酸和猪去氧胆酸含量的测定.     

隆尧鸡腿葱中3种水溶性维生素含量分析

采用高效液相色谱检测鸡腿葱中3种水溶性维生素(VC,VB1,VB2)的含量.以甲醇-0.05 mol/L磷酸二氢钾(pH=6.0)为流动相,进样体积20μL,柱温35℃,流速1.0 mL/min,检测波长270 nm.该方法对3种水溶性维生素的检出限均为0.05 mg/L.在其线性范围内,r达到0.999 0以上.变异系数为0.96％～1.65％,回收率测定结果为85.48％～102.40％.本方法具有准确、成本低等优点,可以用于隆尧鸡腿葱和莱芜鸡腿葱中3种水溶性维生素含量测定.测定结果显示,隆尧鸡腿葱中VC和VB2含量明显高于莱芜鸡腿葱,且有统计学差异,这将为今后隆尧鸡腿葱的开发利用提供技术支持.     

液相色谱法同时测定芩连片中芍药苷、黄芩苷、盐酸巴马汀和盐酸小檗碱的含量

研究并建立了液相色谱法同时测定芩连片中芍药苷、黄芩苷、盐酸巴马汀和盐酸小檗碱含量的新方法.实验条件为Agilent ZorbaxSB—C_(18)柱(250 mm×4.6 mm,5μm),乙腈-0.05%磷酸溶液.梯度洗脱.检测波长为0～8.5 min 230 nm,8.5～23 min 280 nm和23～30 min 346nm.流速1.0 mL/min.芍药苷、黄芩苷、盐酸巴马汀和盐酸小檗碱线性范围分别为3.87～38.72 mg/L、4.18～41.80 mg/L、1.62～16.20 mg/L、2.10～21.00mg/L.应用该法测定芩连片中芍药苷、黄芩苷、盐酸巴马汀和盐酸小檗碱含量.加样回收率分别为100.9%(RSD=2.7%),101.5%(RSD=0.7%),97.9%(RSD=2.0%)和102.2% (RSD=1.6%).     

HPLC-ELSD法测定顺铂脂质体中DSPE-PEG2000含量

为建立以HPLC-ELSD法测定顺铂脂质体中DSPE-PEG2000含量的方法,以Waters Sun Fire prep Silica(250 mm×4.6 mm,5μm)为分离柱(柱温25℃),甲醇-冰醋酸-三乙胺(500∶8∶2,V/V/V)为流动相(流速1 m L/min),蒸发光散射检测器检测(雾化温度50℃,蒸发温度100℃,气体流速1.6 m L/min).结果表明,DSPE-PEG2000在21.08421.6μg/m L浓度范围内线性关系良好(r=0.999 3),加样回收率为99.03%101.23%(RSD=1.08%,n=9).该法能简便、快速、准确地测定顺铂脂质体中DSPE-PEG2000含量,重复性良好.     

苯丙醇的高效液相色谱测定方法研究

运用高效液相色谱法测定苯丙醇的含量,采用C18反相色谱分析柱(4.6×250mm,5μm),流动相为乙腈-H2O(46∶54),流速1.0mL/min,柱温30℃,检测波长257nm.苯丙醇在10~1000mg/mL范围内,线性关系良好(r=0.9991),平均回收率为99.5%.     

新疆戈壁野生木贼麻黄中麻黄碱和盐酸伪麻黄碱含量的测定

采用半仿生提取法提取生物碱,以薄层色谱法(TLC)对戈壁野生木贼麻黄的根、茎与果中提取的生物碱进行了签别,用HPLC同时测定新疆和田皮山戈壁野生木贼麻黄中的盐酸麻黄碱与盐酸伪麻黄碱等生物碱的含量,色谱柱为Agilent HC-C18(4.6 mm×250 mm,5μm),流动相为甲醇-0.2%磷酸水溶液(体积比10∶90),测定波长为210 nm,流速为1.0 m L/min.结果显示,测定盐酸麻黄碱和盐酸伪麻黄碱分别在0.7-4.2μg/m L(r=0.999),0.05-0.3μg/m L(r=0.999)范围内,线性关系良好,平均加标回收率分别为101.6%(RSD=0.3%),102.1%(RSD=0.7%).该法结果准确,重现性好,可用于新疆和田皮山戈壁野生木贼麻黄2种生物碱的含量测定.     

HPLC法同时测定四季三黄丸中盐酸小檗碱、黄芩苷和栀子苷的含量

建立了一种同时测定四季三黄丸中盐酸小檗碱、黄芩苷和栀子苷含量的反相高效液相色谱(RP-HPLC)法.色谱条件为Agilent Zorbax SB-C18分离柱(250 mm×4.6 mm,粒径5μm),乙腈-6 mmol/L KH_2PO_4流动相梯度洗脱,流速1.0m L/min,多波长切换检测.结果表明,盐酸小檗碱、黄芩苷和栀子苷线性范围分别为3.8 28.4μg/m L(r=0.999 8),16.5 123.8μg/m L(r=0.999 6)和3.8 28.4μg/m L(r=0.999 7),加样回收率分别为97.2%(RSD=1.5%),97.7%(RSD=2.6%)和102.9%(RSD=2.8%).本方法可以同时分析四季三黄丸中盐酸小檗碱、黄芩苷和栀子苷含量,对该制剂质量进行有效监控.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…