变色酸2R共振光散射光谱法测定蛋白质

基于人血清白蛋白（HAS）对酸性偶氮染料变色酸2R的共振光散射的增强效应，拟定了一种新的测定HSA的共振光散射法。在pH=4.10的条件下，变色酸2R本身只有极弱的光散射，但它与蛋白质的结合物却有强烈的共振光散射作用，在λex=λcm=420nm处，光散射有最大的散射强度，并且光散射强度与蛋白质HSA的浓度成线性关系，线性范围为0．0－10．0μg/mL，检测限可达0．12μg/ml。该法简便、快速，用于人体血清样品蛋白质的测定并与考马斯亮蓝法比较，结果令人满意。     

邻苯二酚紫共振散射法测定蛋白质含量

基于人血清白蛋白(HSA)对邻苯二酚紫的共振散射的增强效应,拟定了一种新的测定HSA的共振散射法．在pH=4．20的条件下,邻苯二酚紫只有极弱的光散射,但它与蛋白质的结合物却有强烈的共振散射作用,在λ562nm处,光散射有最大的散射强度,并且光散射强度与蛋白质HSA的浓度成线性关系,线性范围为0．0～10．0mg／L,该法简便,快速,用于人血清样品蛋白质的测定,并与用CPA-pA-Ba法测定的结果相比较,结果令人满意．     

萘铬绿与蛋白质作用的共振光散射光谱研究

在pH3 50的酸性介质中,萘铬绿与蛋白质发生静电作用产生以342 0nm为特征峰的共振光散射(RLS)增强光谱.在此波长下,蛋白质的浓度与增强共振光散射强度(ΔIRLS)呈线性关系,据此建立了痕量蛋白质的共振光散射分析法,对BSA和HSA的检测限分别为3 62ng/mL和3 33ng/mL.方法成功地应用于尿样中总蛋白质分析.     

用比布列西猩红共振光散射技术测定蛋白质

在酸性溶液中，比布列西猩红与蛋白质作用，产生共振光散射(RLS)增强光谱，最大散射峰位于286．0nm，且增强RLS强度在一定范围内与蛋白质浓度呈线性关系．研究了pH、比布列西猩红的浓度及离子强度对RLS强度的影响．人血清白蛋白(HSA)的线性范围为0.02～4．0μg／mL，牛血清白蛋(BSA)0．02-4．5μg／mL，溶菌酶(Lys)为0．02-2．0μg／mL，γ-球蛋白(γ-IgG)为0.0l-7.5μg／mL.测定的检测限(3σ)分别为：16．6ng／mL(HSA)，15．7ng／mL(BSA)，16．7ng／mL(Lys)和7．5ng／mL(γ-IgG)．此方法用于人血清样品测定，结果满意．     

共振光散射光谱法测定微量蛋白质

利用光吸收和共振光散射(RLS)光谱研究了铝试剂(ATA)与蛋白质在水溶液中的相互作用.在pH2.50时,蛋白质可使弱的ATA光散射信号曾强.基于这种现象,我们运用RLS技术,建立了测定纳克级蛋白质的方法.该方法简单、实用、灵敏.BSA的线性范围为0.010~27.4μg/mL,HSA的线性范围为0.010~30.5μg/mL.BSA的检测限为10.7 ng/mL,HSA的检测限为10.2 ng/mL.对实际人血清样品中的蛋白质进行了测定,其结果与临床方法一致.氨基酸、金属离子或其它共存化合物的干扰很小.     

共振光散射法测定汞-硫氰酸盐-罗丹明B体系中的汞

研究了汞 (II)与硫氰酸盐和罗丹明B形成离子缔合物的共振光散射的增强效应 ,拟定了一种新的测定汞的共振光散射方法 .通过实验确定了适宜的反应条件以及共振光散射强度与汞 (II)浓度之间的关系 .在λ =6 15nm处 ,共振光散射有最大的散射强度 ,并且共振光散射强度与汞 (II)浓度成线性关系 ,线性范围为 0 .0～ 0 .0 6 0 μg mL ,检测限为 0 .38μg L .该法简便、快速 ,实测水中的汞 (II) ,结果较为满意     

三溴偶氮胂共振光散射法定量测定蛋白质

首次研究了三溴偶氮胂和蛋白质作用的共振光散射光谱．实验发现，在pH3．5～4．7的范围内，蛋白质的加入导致三溴偶氮胂共振光散射增强，并在345和410nm处产生两个增强峰，其强度与蛋白质的浓度成线性关系，据此建立了一种定量测定蛋白质的共振光散射新方法．采用五水平正交设计对反应参数进行选择．方法的线性范围为2．5～30．0μg／mL(BSA)和2．5～40．0μg／mL(HSA)，对应的检测限分别为46．5和43．8ng／mL．该方法已用于实际样品中蛋白质含量的测定，结果令人满意．     

共振光散射比浊法测定胶片废水中银离子的研究

氯化银胶体在375、496和565 nm产生3个明显的共振散射峰,其中496 nm处的散射峰强度最大且最稳定,适于进行共振光散射比浊法分析.探讨了共振光散射比浊法的机理,建立了在酸性氯化钠-乙二醇溶液中,共振光散射比浊法测定银离子的新方法.线性回归方程IRLS=7.60cAg+-4.82,R=0.999 8;检出限0.04μg/mL.此方法用于实际样品测定,取得了满意效果.     

共振光散射法测定亚铁氰化钾的研究

在酸性溶液中,铁离子与亚铁氰化钾反应生成普鲁士蓝,体系的共振光散射强度增强,从而建立了测定亚铁氰化钾的共振光散射光谱法(RLS).在λex=λem=315处,得到一个共振散射峰,其强度与亚铁氰化钾的浓度在4.0～30μg/mL成良好的线性关系,相关系数R为0.999 1,检出限为4.0μg/L,相对标准偏差RSD为1.85%～3.05%.该方法简单快速,灵敏度高,对实际样品进行了测定,得到了令人满意的结果.     

共振光散射比浊法测定环境水样中Pb(Ⅱ)

基于铅与重铬酸钾反应产生沉淀拟定了一种测定铅的共振光散射比浊法.通过实验确定了适宜的反应条件.实验表明,在λ=497 nm处,该体系有较大且较稳定的共振光散射强度,并且共振光散射强度与铅浓度成线性关系,线性范围为0.1～1.0μg/mL,检测限为0.0573μg/mL.该方法快速,灵敏,简便.将该方法用于实际含铅水样中的铅的测定,并与原子吸收光谱法比较,结果较为满意.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.