依诺沙星-铽络合物与脱氧核糖核酸的相互作用研究及其应用

依诺沙星-Tb3+络合物有较强的吸收光潜和荧光光谱,为作为荧光探针研究其与DNA作用机理和荧光增强机理提供了灵敏有效的手段,本文以依诺沙星-铽(Tb3+)作为荧光探针,利用荧光光谱研究了依诺沙星-铽络合物与脱氧核糖核酸(DNA)的相互作用,研究表明,在实验条件下,脱氧核糖核酸能显著增强依诺沙星-Tb3+体系的荧光,据此建立了脱氧核糖核酸的测定方法,线性范围为1.48×10-6～3.84×10-5mol/L,检测限5.2×10-7mol/L.该方法用于核酸样品的测定结果令人满意.同时还探讨了核酸对依诺沙星-Tb3+体系荧光增强效应的作用机理.     

流动注射荧光分光法测定盐酸小蘖碱的研究

研究了十二烷基苯磺酸钠胶束介质中药有效成分盐酸小蘖碱的荧光性质,并应用流动注射技术测定盐酸小蘖碱的浓度,该法线性范围0～9×10-5mol/L,检出限为2.4×10-8mol/L,相对标准偏差为5%,应用于中药样品的测定取得满意的结果.     

萘普生的化学除氧-流体室温燐光法研究

利用化学除氧 -微乳状液增稳室温光法 ( ME- RTP)对萘普生进行了研究 ,比较了萘普生在微乳状液体系和胶束体系中的室温光特性及其影响因素 .建立了测定萘普生的室温光新方法 .结果表明 ,在外重原子( TINO3)诱导下 ,萘普生在微乳状液体系和胶束体系中分别于 5 10 nm和 5 12 nm处有较强的室温光发射 ;相同实验条件下 ,萘普生在微乳状液体系中的 RTP强度比在胶束体系中大 ,且实验条件较宽容 ;在微乳状液体系和胶束体系中 ,萘普生的线性范围分别为 2× 10 - 5mol/L～ 5× 10 - 4 mol/L 和 5× 10 - 6 m ol/L～ 7× 10 - 5mol/L,检出限分别为 2 .1× 10 - 6 mol/L 和 1.9× 10 - 6 mol/L.     

苯并咪唑镧配合物的三维荧光激发和发射光谱

测定了苯并咪唑镧配合物水溶液的紫外光谱、三维荧光激发和发射光谱,讨论了该配合物的升频转换荧光,并发现在4×10-5～5×10-4mol/L范围内290nm处,荧光强度与浓度呈负相关;而在365nm处,浓度1×10-4mol/L时,荧光强度是一极大值。     

化学除氧-胶束增稳室温嶙光法测定维生素K4

在Na2SO3化学除氧的十二烷基硫酸钠(SDS)胶束体系中,以TlAc为重原子微扰剂,进行了维生素K4的室温光法(RTP)测定.研究发现样品温度对RTP发射强度有影响,重原子Tl/SDS有一个临界比率值为40%,到此临界比率值后能获得高的RTP值.介质pH、Na2SO3、SDS及TlAc浓度不仅影响RTP强度,而且影响体系的除氧速度,最佳pH值范围在7.0～7.8之间,分析校正曲线在1×10-6mol/L～8×10-6mol/L和1×10-5mol/L～4×10-5mol/L呈良好的线性关系,方法的检出限(DL)为2.1×10-6mol/L,相对标准偏差(RSD)为4.4%     

锌离子增强荧光光谱法测定谷胱甘肽

探讨了金属离子对还原型谷胱甘肽(GSH)-邻苯二甲醛(OPA)体系荧光信号的影响,实验结果表明Zn2+对体系的荧光信号有增强作用,而且Zn2+能够提高GSH-OPA体系的稳定性,据此建立了一种以Zn2+作为荧光增强剂,快速简便测定还原型谷胱甘肽的新方法.GSH在1.3×10-8～1.4×10-6mol/L的范围内其浓度与体系相对荧光强度有良好的线性关系,检出限为1.1×10-8mol/L.本实验方法比较适合生物样品中还原型谷胱甘肽浓度的测定.     

碘酸钾存在下吡啶的极谱平行催化氢波研究

在0.10mol/L LiCl-8.0×10-4moL/L HCl-4.0×10-4mol/LKIO3支持电解中,吡啶产生一个新的极谱平行催化氢波,峰电位为-1600～-1700mV.该平行催化氢波二阶导数峰电流与吡啶的浓度在8.0×10-5～1.6×10-3mol/L范围呈线性关系,检测下限为2.5×10-5mol/L,可用于吡啶的分析研究.     

溴酸钾存在下苯丙氨酸的极谱催化波研究

在0.10mol/L LiCl-0.02mol/L HCl-8.0×10-3mol/L KBrO3支持电解质中,苯丙氨酸产生一个极谱催化波,峰电位为-1.65V(vs.SCE).该极谱催化波二阶导数峰电流与苯丙氨酸的浓度在2.0×10-5～2.5×10-3mol/L范围呈线性关系,其检测下限为6.5×10-6mol/L.本文建立了苯丙氨酸的分析测定方法,讨论了该极谱催化波的产生机理.     

铽-司帕沙星荧光体系的研究及应用

研究了乙酸 乙酸钠缓冲体系中铽与司帕沙星络合物的荧光特性.司帕沙星与Tb3 存在能量转移关系,使Tb3 545nm处的特征荧光峰大为增强,据此建立了稀土离子Tb3 增敏荧光光谱测定司帕沙星的新方法.测定的最佳条件为:pH5.6,Tb3 浓度4.0×10-4mol/L,λex/λem=275nm/545nm.线性范围1.0×10-7～1.5×10-5mol/L,检测下限1.6×10-9mol/L.该方法用于测定尿液中司帕沙星的含量,结果令人满意.     

酯基Gemini型季铵盐表面活性剂的合成与性能

合成了系列酯基Gemini型季铵盐表面活性剂:烷基α,ω双(二甲基酰氧乙基溴化铵).采用红外光谱和核磁共振进行结构分析;用两相化学滴定法测定活性物含量,结果表明:6种已合成的酯基Gemini型季铵盐表面活性剂的活性物质量分数均在98.5%以上.同时测定了产物的表面活性、泡沫性能和乳化性能,结果表明:酯基Gemini型季铵盐表面活性剂具有较高的表面活性,其临界胶束浓度介于4.46×10-5～4.17×10-4mol/L之间,而相应的单季铵盐表面活性剂C14TABr和C12TABr的临界胶束浓度分别为4.00×10-3mol/L及1.50×10-2mol/L;酯基Gemini型季铵盐表面活性剂的泡沫稳定性及乳化性能也明显优于相应的单季铵盐表面活性剂.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.