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1.
百合基因转化受体系统的建立   总被引:3,自引:0,他引:3  
以百合叶片为外植体不经愈伤组织直接诱导并且发育成带球径的大苗进行了研究,结果表明:叶片分芽培养基为MS+BA0.5mg/L+ZT0.。1mg/L+NAA1.0mg/L生根培养基为1/2MS+NAA0.。2-1.0mg/L,成球并且生长培养基为MS+IAA1.0mg/L,移栽成活率为100%《  相似文献   

2.
播娘蒿子叶及下胚轴诱导再生植珠的研究   总被引:1,自引:0,他引:1  
用播娘蒿种子,在附加6-BA0.5~1.0mg/L+NAA0.1mg/L的MS培养基上获得无菌苗,幼苗的下胚轴在含6-BA0.1mg/L+NAA0.5mg/L的MS培养基上出愈情况最佳,将来自下胚轴的愈伤组织培养于含有6-BA2.0mg/L+NAA0.2mg/L的MS培养基上分化出的丛生芽状态最好,最佳生根培养基为1/2MS+IBA0.5mg/L+NAA0.05mg/L.  相似文献   

3.
百合基因转化受体系统的建立   总被引:2,自引:0,他引:2  
以百合叶片为外植体不经愈伤组织直接诱导并且发育成带球径的大苗进行了研究,结果表明:叶片分芽培养基为MS+BA0.5mg/L+ZT0.1mg/L+NAA1.0mg/L,生根培养基为1/2MS+NAA0.2~1.0mg/L,成球并且生长培养基为MS+IAA1.0mg/L,移栽成活率为100%.从而建立了百合基因转化的受体系统  相似文献   

4.
播娘蒿子叶及下胚轴诱导再生植株的研究   总被引:1,自引:0,他引:1  
用揪娘嵩种子,在附加6-BA0.5~1.0mg/L+NAA0.1mg/L的MS培养基上获得无菌苗,幼苗的下胚轴在含6-BA0.1mg/L+NAA0.5mg/L的MS培养基上的出愈情况最佳,将来自下胚轴的愈伤组织培养了含有6-BA2.0mg/L+NAA0.2mg/L的MS培养基上分化出的丛生芽状态最好,最佳生根培养基为1/2MS+IBA0.5mg/L+NAA0.05mg/L。  相似文献   

5.
风信子外植体植株再生系统的研究   总被引:8,自引:1,他引:7  
风信子叶片及鳞片直接诱导下定芽的培养基为MS附加6-BA2.5mg/L、NAA2.0mg/L、IAA0.5mg/L和MS附加6-BA2.5mg/L、NAA2.5mg/L。不定芽发育成小鳞茎的培养基为MS附加GA0.2mg/L、IAA0.5mg/L。鳞茎移栽成活率为90%,从而建立了风信子植株再生系统。  相似文献   

6.
郁金香叶片直接诱导试管人工种球的快速繁殖的研究   总被引:2,自引:0,他引:2  
对郁金香的叶片直接诱导试管人工种球及发育成大球的快速繁殖途径进行了研究,结果表明:叶段在MS+BA2.0mg/L+NAA2.0 ̄5.0mg/L中不经愈伤组织直接分化多个小鳞茎,Φ(Φ:鳞茎的直径)2mm的小鳞茎在MS+GA1.0 ̄2.0mg/L中发育成带1 ̄2片叶的Φ5 ̄10mm的大鳞茎,大鳞茎的移栽成活率为98% ̄100%。从而建立了郁金香试管人工种球快速繁殖的新途径。  相似文献   

7.
雪兔子叶的组织培养及植株再生   总被引:1,自引:0,他引:1  
以雪兔子无菌苗的幼叶为外植体,接种到MS补加NAA(1.0-2.0)mg/L、6-BA(0.1-1.0)mg/L的培养基上诱导愈伤组织,其中以MS+NAA2.0mg/L+6-BA0.5mg/L的诱导效果最好,经6周培养即可形成大量的黄绿色愈伤组织,诱导率可达100%,经2-3次继代培养后,将生长发良好的愈伤组织转接到MS附加6-BA(0.5-2.0)mg/L、IAA(0.05-0.1)mg/L、N  相似文献   

8.
百合子房的组织培养   总被引:9,自引:0,他引:9  
以百合子房为材料进行组培快繁,得到子房诱导成愈伤组织的最适培养基为MS+BA1mg/L+NAA0.5mg/L:适合诱导分化的培养基为MS+BA0.5mg/L+IAA0.5mg/L;适合生根的培养基为MS+BA0.2mg/L+IAA1mg/L.  相似文献   

9.
温桂珍 《广西科学》1995,2(2):62-63
以巴西铁(Dracaena sanderiena)茎段为接种材料,MS为基本培养基,吲哚乙酸(IAA)、茶乙酸(NAA),2,4-二氯苯氧乙酸(2.4-D)、6-苄氨基嘌呤(6-BA)、6一糠基氨基嘌呤(6-FA)、吲哚丁酸(IBA)、生根粉(ABT1)、玉米素(ZT)为附加成分,进行诱导、分化、生根试验。诱导结果表明:IAA不起作用,2,4-D有较好的促进作用,0.5mg/L(2,4-D)+2mg/L(6-BA)组合,诱导率高达90%。分化结果表明:NAA、6-BA效果较好,6-FA作用不明显,lmg/LNAA+4ms/L(6-BA)+2ms/LZT组合,分化率最高.达81.1%。生根结果表明:MS+0.5mg/LNAA+0.5mg/L(6-FA),MS+0.4mg/LNAA+2mg/LIBA,MS+0.2mg/LNAA+0.1mg/L(6-FA)+ABT1三种培养基均发根,生根率70%以上,根粗,但有点脆,若培养基中加入活性碳产生的根则细而韧。  相似文献   

10.
以MS为基本培养基,附加不同种类、不同浓度的细胞分裂素(BA,6-苄基腺嘌呤,ZT,玉米素)和生长素(NAA,荼乙酸),对月季组培快速繁殖进行了研究。结果表明:在萌芽率上均以较低浓度的细胞分裂素与生长素按一定比例混合使用效果较好,适宜的培养基为MS+BA1mg/L+NAA0.2mg/L.而在成芽率上以较高浓度的细胞分裂素单独使用效果最好,适宜的培养基为MS+ZT2mg/L。  相似文献   

11.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

12.
AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…  相似文献   

13.
本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。  相似文献   

14.
Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with  相似文献   

15.
The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to  相似文献   

16.
17.
The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.  相似文献   

18.
R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).  相似文献   

19.
The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.  相似文献   

20.
Advances in wireless technologies and positioning technologies and spread of wireless devices, an interest in LBS (Location Based Service) is arising. To provide location based service, tracking data should have been stored in moving object database management system (called MODBMS) with proper policies and managed efficiently. So the methods which acquire the location information at regular time intervals then, store and manage have been studied. In this paper, we suggest tracking data management techniques using topology that is corresponding to the moving path of moving object. In our techniques, we update the MODBMS when moving object arrived at a street intersection or a curved road which is represented as the node in topology and predict the location at past and future with attribute of topology and linear function. In this technique, location data that are corresponding to the node in topology are stored, thus reduce the number of update and amount of data. Also in case predicting the location,because topology are used as well as existing location information, accuracy for prediction is increased than applying linear function or spline function.  相似文献   

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