氟罗沙星在胶束体系中的荧光特性研究及应用

目的:研究氟罗沙星在胶束体系中的荧光特性及应用.方法:用日立F-3010型荧光分光光度计测定,测定波长eλx=290 nm,eλm=450 nm,测定时最佳pH值范围为4.5~5.5.在表面活性剂十二烷基硫酸钠(SDS)存在下,由于胶束的生成,使氟罗沙星(FLRX)自身荧光大大增强,据此建立了在SDS胶束体系中测定氟罗沙星的新方法.结果:氟罗沙星浓度在0~0.9μg/mL范围内与荧光强度呈线性关系,相关系数0.9991,检测限为0.01μg.mL-1.加标回收率为97.1%~102.5%,相对标准偏差为1.5%~3.6%.结论:该方法简便,快速,可直接用于药物制剂中氟罗沙星含量的测定.     

显色剂2-(5-羧基-1,3,4-三氮唑偶氮)-5-二乙氨基苯胺与铁的显色反应

研究了显色剂2-(5-羧基-1,3,4-三氮唑偶氮)-5-二乙氨基苯胺(简称CTZAN)与铁的显色反应.结果表明,在pH 6.0的HAc-NaAc缓冲体系中,CTZAN与Fe(Ⅲ)形成一种稳定的络合比为2:1的紫红色络合物,最大吸收波长位于570 nm处,络合物的表观摩尔吸光系数为2.08×104 L·mol-1·cm-1,Fe(Ⅲ)质量浓度在(0.08～0.8)μg/ mL范围内符合比尔定律.所拟方法不经分离直接测定纯铝和铝合金样品中微量的铁,测定结果与认定值相符.     

氟罗沙星在胶束体系中的荧光特性

研究了氟罗沙星(FLRX)在胶束体系中的荧光性质,发现在酸性介质中十二烷基硫酸钠(SDS)对氟罗沙星有较强的增敏作用,据此建立了胶束增敏荧光光谱法测定氟罗沙星的新方法。在0.11μg·mL-1～5.5μg·mL-1范围内,FLRX的浓度与荧光强度呈良好的线性关系,检出限为0.07μg·mL-1。经加样回收实验,回收率为97.4%～107.2%,相对标准偏差为2.0%～4.6%。     

新显色剂2-（5-羧基-1，3，4-三氮唑偶氮）-5-二乙氨基苯甲酸与铁的显色反应

研究了新显色剂2-(5-羧基-1,3,4-三氮唑偶氮)-5-二乙氨基苯甲酸(简称CTZDBA)与铁的显色反应。结果表明,在pH4.0的HAc-NaAc缓冲溶液中,CTZDBA与Fe(Ⅲ)形成稳定的络合比为2∶1的紫红色络合物,最大吸收波长为560nm,络合物的表观摩尔吸光系数为2.21×104L.mol-1.cm-1,Fe(Ⅲ)质量浓度在(0～0.8)mg/L范围内符合比尔定律。所拟方法不经分离直接测定纯铝和铝合金样品中微量的铁,测定结果与认定值相符。     

非离子表面活性剂Tween-80存在下5-Br-PADAP分光光度法同时测定微量Cu(Ⅱ)和Fe(Ⅲ)

在pH 3.50酸性介质中,Cu(Ⅱ)和Fe(Ⅲ)在非离子表面活性剂Tween-80存在下与5-Br-PADAP生成有色配合物.有色配合物最大吸收波长都位于570 nm处;表观摩尔吸光系数分别为7.96×104 L·mol-1·cm-1和6.30×104 L·mol-1·cm-1;Cu(Ⅱ)在0～13 μg/25ml,Fe(Ⅲ)在0～18 μg/25ml范围内符合比耳定律.用拟定的方法测定了铝片和水样中的Cu(Ⅱ)和Fe(Ⅲ),结果令人满意.     

氟罗沙星和培氟沙星贯序治疗下呼吸道感染的成本-效果分析

目的: 对氟罗沙星和培氟沙星贯序治疗下呼吸道感染进行药物经济学分析, 为临床提供用药参考. 方法采用回顾性研究法, 收集122例下呼吸道感染患者, 分成两个治疗组:氟罗沙星组和培氟沙星组, 采用统计研究和成本-效果分析方法对两治疗组进行药物经济学比较. 结果氟罗沙星和培氟沙星贯序治疗下呼吸道感染总有效率分别为89.83%和87.30%, 效果无显著差异(P>0. 05);治疗成本分别为290.63元和236.07元, 成本-效果比分别为3.23和2.70. 结论与氟罗沙星相比, 培氟沙星贯序治疗下呼吸道感染具有成本-效果优势.     

Fe(Ⅲ)-邻氯苯基荧光酮络合吸附波研究

在pH值为10.3的NH3H2O NH4Cl介质中,Fe(Ⅲ)与邻氯苯基荧光酮(以下简称o CPF)生成络合物,于-0.72V(vs.SCE)出现一灵敏的极谱波.铁(Ⅲ)含量在1.0×10-8～1.3×10-6mol/L范围内与峰高呈线性关系,检出限为4.0×10-9mol/L.用多种电化学方法研究了极谱波的性质及电极反应机理,证明-0.72V处的极谱波为络合吸附波,峰电流由中心离子Fe(Ⅲ)还原产生,络合物组成为Fe(Ⅲ)∶o CPF=1∶2,表观稳定常数K稳=5.4×1012.试验了30多种离子对峰电流的影响,并将该体系用于头发中痕量铁的测定.     

荧光法研究氟罗沙星与牛血清白蛋白的相互作用

用荧光法研究了氟罗沙星对血清白蛋白的荧光猝灭现象，根据荧光猝灭双倒数图读物计算了氟罗沙星与牛血清白蛋白之间的结合常数，根据Foester能量传递原理计算出氟罗沙星在牛血清白蛋白上的结合位置，并根据热力学参数研究了氟罗沙星与牛血清白蛋白之间的作用力类型。     

分光光度法测定头孢曲松钠的含量

通过研究头孢曲松钠与Fe3 的反应并确定其反应的最佳条件,从而建立新的分光光度测定法。头孢曲松钠与Fe3 在常温下能够立即形成稳定的络合物,该络合物在470nm处有最大吸收,表观摩尔吸光系数为1.5×103L/(mol·cm),线性范围为0.05～0.60mg/L.用该法测定头孢曲松钠药品,结果与中国药典法基本吻合,相对标准偏差为0.63%,回收率为99.2%～99.5%.     

2-(2-喹啉偶氮)-1,5-苯二酚光度法测定烟草中铁的研究

合成了新试剂2-(2-喹啉偶氮)-1,5-苯二酚(QADHB),研究了2-(2-喹啉偶氮)-1,5-苯二酚与铁(Ⅲ)的显色反应在pH=7.0的HAC-NaAC缓冲溶液介质中,十六烷基三甲基溴化胺(CTMAB)存在下,QADHB与Fe(Ⅲ)形成红褐色稳定2∶1络合物,λmax=580nm,ε＝2.37×104L.mol-1.cm-1.Fe3+含量在0～20μg/25mL内符号比尔定理,方法用于烟草中铁含量的测定,结果令人满意.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.