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1.
以野生蜗牛嗉囊为原料提取蜗牛酶产品,检测所含纤维素酶、淀粉酶的活性,对其蜗牛酶产品进一步测试,结果表明新疆蜗牛酶中的纤维素酶活力为450U/g淀粉酶为405U/g。验证了新疆蜗牛酶中纤维素酶的活力很高。  相似文献   

2.
应用响应面法优化酶法提取白背毛木耳子实体多糖的工艺条件。使用纤维素酶辅助进行白背毛木耳子实体多糖的提取,在单因素试验的基础上通过响应面试验设计构建了模型并确定酶用量、pH值、提取温度、提取时间等4个主要影响因素的最佳工艺参数。结果表明,最佳的工艺条件为:纤维素酶用量3 774 U/g、pH为4.9、提取温度50.2℃、提取时间135 min,在此条件下白背毛木耳子实体多糖的提取率为15.47%,与模型预测值15.52%的相对误差仅为0.323%。  相似文献   

3.
酶法提取金针菇多糖   总被引:11,自引:0,他引:11  
余冬生  王文高 《应用科技》2001,28(10):56-57,52
研究了金针菇多糖提取的最佳工艺条件。单因素实验确定了纤维素酶,菠萝蛋白酶提取的最佳工艺条件,在此基础上进行了复合酶正交实验的研究,结果得出最佳工艺条件是时间80min,温度50℃,pH值4.0,纤维素酶1.0%,菠萝蛋白酶1.0%,果胶酶1.0%。  相似文献   

4.
确定了复合酶(纤维素酶、果胶酶、中性蛋白酶)提取蕨麻多糖的最佳工艺,并对其体外抗氧化活性进行初步研究.在单因素试验的基础上,设计L9 (33)正交实验和L9(34)正交实验,分别得出复合酶的最佳配比和复合酶法提取蕨麻多糖的最佳提取工艺条件;采用清除·OH(羟基)自由基模型和O2-·(超氧阴离子)自由基模型评价了蕨麻多糖的抗氧化能力.结果表明:复合酶的最佳配比为:纤维素酶2.0%,木瓜蛋白酶1.0%,果胶酶2.0%;最佳工艺条件为∶料液比为1∶30、pH值为4.5,温度为45℃,酶解时间为60min,此时蕨麻多糖的提取率最大为8.12%,同时验证性实验也表明优化得到的提取工艺稳定可靠;蕨麻多糖对羟基自由基和超氧阴离子都具有较强的清除作用,并与浓度呈一定依赖关系,且对羟自由基的清除能力要比超氧阴离子自由基的清除能力强.  相似文献   

5.
酶法提取针叶胶   总被引:2,自引:0,他引:2  
以松针为材料,用正交实验分析了影响纤维素酶活力的三个主要因素,并根据正交实验结果,对单个因素的多个水平进行了比较分析,结果表明:温度是影响纤维素酶提取针叶胶的最主要因素;酶用量高于20U/g以后,对针叶胶提取率影响不大;在温度50℃、pH5.2、酶用量20 U/g条件下,用纤维素酶处理后,其提取针叶胶的提取率最高.  相似文献   

6.
新疆棉秆资源丰富,将棉秆酶解生成可发酵糖,有利于其高值化利用。本研究以棉秆为原料,将其经过Na OH处理后,以纤维素酶用量、酶解温度、水解时间和固含量为输入参数,以酶解得到的还原糖产率为目标输出,在Box-Behnken设计实验的基础上,采用BP神经网络对经Na OH处理后的棉秆在纤维素酶中酶解的过程进行模拟与优化,建立了棉秆在纤维素酶中酶解糖化的神经网络模型,优化了棉秆在纤维素酶中酶解的工艺。模型分析结果表明:优化的神经网络模型均方误差小,回归值为0.9571,网络性能稳定,预测结果准确。通过模型优化获得最优的酶解条件为:酶用量为70 FPU/g,温度为46.31℃,酶解时间为72 h和固含量为7.5%,获得还原糖产率最高为62.14%;XRD、SEM和FT-IR的分析结果表明:Na OH预处理能有效除去木质素,使纤维素的含量相对升高,与纤维素酶的接触位点增多,从而提高棉秆的水解产率。  相似文献   

7.
利用表面活性剂与酶协同超声波法提取红藤中的总黄酮,以得率和抗氧化性为指标,考察表面活性剂的种类和用量、酶的种类和用量、提取温度和时间、溶剂pH、液固比等因素的影响 ;在单因素实验基础上运用正交试验确定最佳工艺.结果表明:以得率为指标的最佳工艺条件为提取时间70 min,液固比30 mL/g,纤维素酶用量0.5mg/mL,得率达到7.98% ;以抗氧化性为指标的最佳工艺条件为提取时间70 min,液固比50 mL/g,纤维素酶用量0.5 mg/mL,此时自由基清除率为67.56%.  相似文献   

8.
以干柚皮为原料,研究了纤维素酶辅助提取柚皮中果胶的工艺.分别讨论了纤维素酶用量、时间、温度和料液比等因素对果胶提取率的影响,并通过单因素及L9(34)正交试验,确定了当pH=2.0时从柚皮中提取果胶的最佳工艺条件为:纤维素酶用量0.15%,料液比1∶40,提取时间3 h,提取温度45℃,在此条件下果胶提取率达到6.02%.  相似文献   

9.
采用蛋白酶、果胶酶、纤维素酶复合处理冬瓜多糖溶液,通过对复合酶的作用温度、作用pH值、作用时间进行单因素实验,对复合酶配比、复合酶的提取工艺进行正交试验,确定了复合酶法提取冬瓜多糖的最佳工艺条件:复合酶按纤维素酶1.5%,果胶酶1.5%,蛋白酶1.5%配比,酶作用温度40℃,酶作用pH值为5.0,酶作用时间为40 min,多糖提取率最高.  相似文献   

10.
以漳州血柚皮为原料,利用纤维素酶对血柚皮总黄酮进行提取,并以硝酸铝显色法测定总黄酮提取率.分别对酶用量、pH值、酶解温度、酶解时间进行单因素实验和正交试验,并通过极差、方差分析对提取过程显著影响提取率的因素进行统计分析.结果表明,纤维素酶提取血柚皮中的总黄酮的优选工艺条件为:酶用量5.5%,pH 5.2,酶解温度52℃,酶解时间为50 min,该工艺条件下血柚皮总黄酮的提取率可达1.52%.  相似文献   

11.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

12.
AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…  相似文献   

13.
本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。  相似文献   

14.
Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with  相似文献   

15.
The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to  相似文献   

16.
17.
The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.  相似文献   

18.
R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).  相似文献   

19.
The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.  相似文献   

20.
Advances in wireless technologies and positioning technologies and spread of wireless devices, an interest in LBS (Location Based Service) is arising. To provide location based service, tracking data should have been stored in moving object database management system (called MODBMS) with proper policies and managed efficiently. So the methods which acquire the location information at regular time intervals then, store and manage have been studied. In this paper, we suggest tracking data management techniques using topology that is corresponding to the moving path of moving object. In our techniques, we update the MODBMS when moving object arrived at a street intersection or a curved road which is represented as the node in topology and predict the location at past and future with attribute of topology and linear function. In this technique, location data that are corresponding to the node in topology are stored, thus reduce the number of update and amount of data. Also in case predicting the location,because topology are used as well as existing location information, accuracy for prediction is increased than applying linear function or spline function.  相似文献   

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