成熟猕猴桃果实不同组织中ACC氧化酶基因的表达差异研究

了猕猴桃“海沃特”成熟果实各组织中ACC氧化酶基因的转录水平和翻译水平对乙烯生成的影响，结果表明：（1）乙烯生成具有组织差异性；（2）ACC氧化酶基因mRNA的表达在各组中水平相似；（3）ACC氧化酶的表达水平具有显著的组织差异性及时间顺序性，因此，ACC氧化酶的瑶达水平的高低可能是导致乙烯生成的组织差异性的主要原因之一。     

番茄ACC合酶(LeACS2)在大肠杆菌中的可溶性表达

植物激素乙烯参与了植物生长发育等一系列生理过程,通过控制乙烯的合成进而控制经济作物的衰老和果实的成熟,给农业带来深远影响.ACC合酶是高等植物乙烯生物合成的限速酶.通过RT-PCR将番茄ACC合酶(LeACS2)cDNA克隆至大肠杆菌表达载体pET30a中并使之与GST 编码序列进行融合而构建了重组表达质粒pET-LeACS2-GST.转化获得含有该重组表达质粒的大肠杆菌BL21 starTM(DE3) pLysS细胞,在IPTG的诱导下成功表达出含有LeACS2的大小约83 000的融合蛋白.该融合蛋白具有较好的可溶性.通过GST亲和层析和MonoQ阴离子交换两步纯化后的LeACS2能够催化其底物SAM生成ACC.     

中华猕猴桃成熟软化过程中果胶酶（PE、PG）活性的测定

研究中华猕猴桃两个品种果实成熟过程中，果胶酶PE、PG的变化发现：在果实成熟软化过程中，PE、PG活性越高、协同作用越强，果实软化越快，PG活性在果实软化中的作用更大。     

猕猴桃AsA生物合成途径及调控机制研究进展

抗坏血酸(AsA)在植物生长发育及果实品质形成方面扮演重要角色,对人体健康也具有重要作用.猕猴桃果实中富含AsA,是研究植物AsA代谢与调控的宝贵材料.目前对于AsA生物合成已经有一定的了解,植物中公认的AsA生物合成途径有4种,其中对主要合成途径L-半乳糖途径研究比较透彻,其他3条途径仍有未证实的酶反应和相关基因,AsA代谢及调控机理也有待深入研究.综合国内外AsA研究现状,主要从猕猴桃中AsA积累的差异、AsA的生物合成及其相关基因与调控酶进行阐述,以期为今后通过植物生物合成途径提高AsA含量提供理论依据,为猕猴桃种质创新与品种改良奠定基础.     

内源多胺对梨果实生长以及乙烯生物合成的调节作用

该项目研究了不同品种的梨果实生长过程中内源多胺的含量变化和梨果实不同组织在采收前后的内源多胺、EFE、ACC以及内源乙烯的含量变化,明确了梨果实中存在着3中内源多胺,分析了不同种类的多胺对梨果实生长和内源乙烯的生物合成的不同调节作用,并发现不同品种梨果实对乙烯的敏感性存在着显著的差异,同时改进了对乙烯的分离浓缩技术和ACC的快速测定技术.     

溶菌酶结合纳米包装对猕猴桃贮藏品质的影响

研究溶菌酶结合纳米包装对猕猴桃果实贮藏期品质的影响。以未经过处理的猕猴桃果实为对照样品,探讨了溶菌酶结合纳米包装处理对猕猴桃果实采后贮藏期间呼吸强度、乙烯释放量、腐烂率、失重率、抗坏血酸过氧化物酶和超氧化物歧化酶的影响。实验结果表明,与对照相比,溶菌酶和纳米包装处理能有效抑制猕猴桃的呼吸强度、乙烯释放量、腐烂率和失重率的上升,提高抗坏血酸过氧化物酶和超氧化物歧化酶的活性;其中,溶菌酶结合纳米包装联合处理对提高猕猴桃的贮藏期品质更显著。由此可见,溶菌酶和纳米包装处理能不同程度地延缓猕猴桃果实的后熟和衰老,并提高了猕猴桃果实的品质和价值,为溶菌酶和纳米包装技术在果蔬采后贮藏保鲜应用中提供理论基础。     

中华猕猴桃果实青霉病抗性差异研究

青霉菌引起的猕猴桃果实腐烂是猕猴桃果实成熟和采后贮藏中的最主要病害之一,为了研究中华猕猴桃对青霉病抗性的差异,文章以中华猕猴桃中的红阳、翠玉和16A 3个品种为材料,研究了各品种在受到青霉菌侵染后菌斑大小以及抗性相关生理指标的变化,包括丙二醛(MDA)、过氧化物酶(POD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)4种酶在青霉菌侵染后不同时期酶活力的变化。结果表明,POD、CAT及PAL的量变化趋势从大到小依次为翠玉、16A、红阳,而MDA的量从小到大依次为翠玉、16A、红阳。综合菌斑大小的变化,该研究表明翠玉对青霉病抗性最大,其次为16A,红阳的抗病性最低。POD、CAT及PAL量的升高可以提高果实对青霉菌的抗病性。该研究结果为深入研究猕猴桃青霉病和提高青霉病抗性的研究提供了理论基础。     

ACC氧化酶基因研究进展

乙烯作为植物的五大类激素之一,在植物的生长发育过程中发挥着重要作用.而乙烯在植物体内的生物合成主要是由ACC合成酶和ACC氧化酶这2个限速酶来控制的,通过调节它们的表达能有效地调节乙烯的合成量.本文就ACC氧化酶基因克隆及表达调控进行了综述,并对其应用前景进行了展望.     

番茄果实成熟过程中色素合成相关基因的表达分析

对番茄果实成熟过程中色素含量进行检测,并利用实时定量PCR技术对色素合成相关基因的表达进行分析.结果表明,番茄果实成熟过程中,叶绿素含量逐渐降低,花青素含量变化不显著,番茄红素及类黄酮含量显著升高,大部分调控色素合成的转录因子基因、类胡萝卜素代谢途径以及类黄酮代谢途径相关基因在果实破色后呈现先上升后下降的趋势.     

ACC含量、EFE活性在甜瓜乙烯生成中的变化与乙烯生成限速的初探

对河套蜜瓜乙烯生成中ＩＥＣ、ＡＣＣ含量、ＥＦＥ活性研究表明，三者变化趋势一致．其中ＡＣＣ含量峰值与ＩＥＣ峰值同时出现；ＥＦＥ活性升高与峰值出现先于ＡＣＣ含量．乙烯利处理呼吸跃变前后果实试验表明，外源乙烯可启动和促进乙烯的合成，ＥＦＥ和ＡＣＣ合酶都为该种甜瓜乙烯合成中的诱导酶和限速酶．利用果胶降解物处理果实的初步研究表明，果胶降解物可明显促进ＥＦＥ活性，但没有测到ＩＥＣ．     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.     

Tracking data management using topology of moving object database system for moving object constrained trajectory in LBS application

Advances in wireless technologies and positioning technologies and spread of wireless devices, an interest in LBS (Location Based Service) is arising. To provide location based service, tracking data should have been stored in moving object database management system (called MODBMS) with proper policies and managed efficiently. So the methods which acquire the location information at regular time intervals then, store and manage have been studied. In this paper, we suggest tracking data management techniques using topology that is corresponding to the moving path of moving object. In our techniques, we update the MODBMS when moving object arrived at a street intersection or a curved road which is represented as the node in topology and predict the location at past and future with attribute of topology and linear function. In this technique, location data that are corresponding to the node in topology are stored, thus reduce the number of update and amount of data. Also in case predicting the location,because topology are used as well as existing location information, accuracy for prediction is increased than applying linear function or spline function.