共查询到20条相似文献,搜索用时 31 毫秒
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The developmental transcriptome of Drosophila melanogaster 总被引:2,自引:0,他引:2
Graveley BR Brooks AN Carlson JW Duff MO Landolin JM Yang L Artieri CG van Baren MJ Boley N Booth BW Brown JB Cherbas L Davis CA Dobin A Li R Lin W Malone JH Mattiuzzo NR Miller D Sturgill D Tuch BB Zaleski C Zhang D Blanchette M Dudoit S Eads B Green RE Hammonds A Jiang L Kapranov P Langton L Perrimon N Sandler JE Wan KH Willingham A Zhang Y Zou Y Andrews J Bickel PJ Brenner SE Brent MR Cherbas P Gingeras TR Hoskins RA Kaufman TC Oliver B Celniker SE 《Nature》2011,471(7339):473-479
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Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs 总被引:6,自引:0,他引:6
Okazaki Y Furuno M Kasukawa T Adachi J Bono H Kondo S Nikaido I Osato N Saito R Suzuki H Yamanaka I Kiyosawa H Yagi K Tomaru Y Hasegawa Y Nogami A Schönbach C Gojobori T Baldarelli R Hill DP Bult C Hume DA Quackenbush J Schriml LM Kanapin A Matsuda H Batalov S Beisel KW Blake JA Bradt D Brusic V Chothia C Corbani LE Cousins S Dalla E Dragani TA Fletcher CF Forrest A Frazer KS Gaasterland T Gariboldi M Gissi C Godzik A Gough J Grimmond S Gustincich S Hirokawa N Jackson IJ Jarvis ED Kanai A 《Nature》2002,420(6915):563-573
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Alternative isoform regulation in human tissue transcriptomes 总被引:13,自引:0,他引:13
Wang ET Sandberg R Luo S Khrebtukova I Zhang L Mayr C Kingsmore SF Schroth GP Burge CB 《Nature》2008,456(7221):470-476
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Modular regulatory principles of large non-coding RNAs 总被引:3,自引:0,他引:3
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Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project 总被引:2,自引:0,他引:2
ENCODE Project Consortium Birney E Stamatoyannopoulos JA Dutta A Guigó R Gingeras TR Margulies EH Weng Z Snyder M Dermitzakis ET Thurman RE Kuehn MS Taylor CM Neph S Koch CM Asthana S Malhotra A Adzhubei I Greenbaum JA Andrews RM Flicek P Boyle PJ Cao H Carter NP Clelland GK Davis S Day N Dhami P Dillon SC Dorschner MO Fiegler H Giresi PG Goldy J Hawrylycz M Haydock A Humbert R James KD Johnson BE Johnson EM Frum TT Rosenzweig ER Karnani N Lee K Lefebvre GC Navas PA Neri F Parker SC Sabo PJ 《Nature》2007,447(7146):799-816
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Jaillon O Bouhouche K Gout JF Aury JM Noel B Saudemont B Nowacki M Serrano V Porcel BM Ségurens B Le Mouël A Lepère G Schächter V Bétermier M Cohen J Wincker P Sperling L Duret L Meyer E 《Nature》2008,451(7176):359-362
Most eukaryotic genes are interrupted by non-coding introns that must be accurately removed from pre-messenger RNAs to produce translatable mRNAs. Splicing is guided locally by short conserved sequences, but genes typically contain many potential splice sites, and the mechanisms specifying the correct sites remain poorly understood. In most organisms, short introns recognized by the intron definition mechanism cannot be efficiently predicted solely on the basis of sequence motifs. In multicellular eukaryotes, long introns are recognized through exon definition and most genes produce multiple mRNA variants through alternative splicing. The nonsense-mediated mRNA decay (NMD) pathway may further shape the observed sets of variants by selectively degrading those containing premature termination codons, which are frequently produced in mammals. Here we show that the tiny introns of the ciliate Paramecium tetraurelia are under strong selective pressure to cause premature termination of mRNA translation in the event of intron retention, and that the same bias is observed among the short introns of plants, fungi and animals. By knocking down the two P. tetraurelia genes encoding UPF1, a protein that is crucial in NMD, we show that the intrinsic efficiency of splicing varies widely among introns and that NMD activity can significantly reduce the fraction of unspliced mRNAs. The results suggest that, independently of alternative splicing, species with large intron numbers universally rely on NMD to compensate for suboptimal splicing efficiency and accuracy. 相似文献
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Alternative pre-mRNA splicing and proteome expansion in metazoans 总被引:77,自引:0,他引:77
The protein coding sequences of most eukaryotic messenger RNA precursors (pre-mRNAs) are interrupted by non-coding sequences called introns. Pre-mRNA splicing is the process by which introns are removed and the protein coding elements assembled into mature mRNAs. Alternative pre-mRNA splicing selectively joins different protein coding elements to form mRNAs that encode proteins with distinct functions, and is therefore an important source of protein diversity. The elaboration of this mechanism may have had a significant role in the expansion of metazoan proteomes during evolution. 相似文献
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The organization of genes into exons separated by introns may permit rapid evolution of protein-coding sequences by exon shuffling. Introns could provide non-coding targets for recombination, which would then give rise to novel combinations of exons. Evidence to support this theory is indirect and consists of examples of homologous domains of protein structure encoded in different genes, with introns in conserved positions at the boundaries of these domains. Here, we report the first direct evidence for exon shuffling. Two spontaneous deletion mutations of phage T4 have been characterized by sequencing, and they are clearly the result of recombination between homologous regions of two self-splicing group I introns. As a result of the recombination, exons of different genes are transcribed together, with a hybrid intron between them. One of these introns is proficient in self-splicing. 相似文献
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Structure and transcription of human papillomavirus sequences in cervical carcinoma cells 总被引:36,自引:0,他引:36
E Schwarz U K Freese L Gissmann W Mayer B Roggenbuck A Stremlau H zur Hausen 《Nature》1985,314(6006):111-114
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Identifier sequences are transcribed specifically in brain 总被引:4,自引:0,他引:4