PVS患者血液中ET和NO的含量变化及临床意义

采用放射免疫法和硝酸还原酶法 ,分别对持续性植物状态 (PVS)患者血中内皮素 (ET)和一氧化氮(NO)含量进行测定 .结果表明 ,PVS患者血浆ET为 12 9.9± 6 1.92mg/mL ,显著高于对照组 72 .0± 2 1.73mg/mL(P<0 .0 0 5 ) ;PVS患者血清NO为 130 .889± 91.834 μmol/L ,显著高于对照组 72 .336± 6 0 .2 12 μmol/L(P <0 .0 5 ) .说明PVS患者血中ET和NO的含量异常与PVS的发生与发展有关 ,可为PVS的临床诊断提供参考 .     

~1Rh(Ⅲ)对鲁米诺-H_2O_2-Cu(Ⅱ)化学发光抑制作用的研究及其测定

研究 Rh( )对 Lu- H2 O2 - Cu( )化学发光体系的抑制作用 ,提出在贵金属混合物中测定微量 Rh( )的新方法 :在 p H=1 2 .40～ 1 2 .6 0的碱性溶液中 ,微量 Rh( )可抑制 ( 2 .5～ 7.5)× 1 0 -3 mol/ L Lu- ( 1～ 4)× 1 0 -2 mol/ L H2 O2 - Cu( ) (与待测 Rh在同一数量级 )体系的化学发光 ,线性范围 0 .1～ 1 0μg/ m L Rh( ) ,相对标准偏差 2 % ,检出限 0 .0 4 μg/ m L Rh( ) ,已用于 7种贵金属人工混合试液和 Pt- Rh合金中 Rh的测定 .     

Baclofen对大鼠新鲜分离DRG神经元GABA和NMDA激活电流的抑制作用

用全细胞膜片箝技术在大鼠新鲜分离的背根神经节 (DRG)细胞上观察到baclofen对GABA和NMDA激活电流有调制作用。单独给予DRG细胞baclofen (1～ 10 0 μmol/L)未记录到可测的电流改变 ,但预加baclofen对GABA和NMDA激活电流具有明显的抑制作用 ,且呈剂量依赖性。 10 0 μmol/Lbaclofen对GABA和NMDA激活电流分别抑制(5 1.2± 10 .9) % (X±SE ,n=8,P <0 .0 1)和 (6 4 .1± 2 1.1) % (X±SE ,n=6 ,P <0 .0 1) ,此抑制作用是可逆的 ,可被GABAB 受体拮抗剂saclofen(10 0 μmol/L ) 所取消 (n =4 )。     

脐血铅含量与新生儿神经行为发育的关系

随机选取第一冶金建设公司职工医院妇产科收治的1999年1～10月出生的足月新生儿103例,以脐血铅(CBPb)含量作为新生儿宫内铅暴露的指标,新生儿神经行为评分作为效应指标,采用描述性分析方法评价其相关关系.脐血铅水平在0.3885±0.1683pmol/L(x±S),脐血铅以0.483μmol/L为界,分为高铅组与低铅组,发现仅在新生儿神经行为测定总评分(NBNA)(X2=4.0456p＜0.05)、生物性视听定向反应(BVA0R)(X2=6.3133 p＜0.05)方面两组间神经评分差异有显著性意义,但脐血铅与新生儿神经行为评分之间等级相关关系无统计学意义(p＞0.05).结果表明低水平铅暴露对新生儿神经行为的发育,尤其是对新生儿视听功能的发育,可产生不利影响.     

O型孕妇血清IgG抗体效价与新生儿溶血病和高胆红素血症发生的关系

目的:探讨O型孕妇血型抗体效价与新生儿溶血病和高胆红素血症发生以及蓝光治疗时间关系。方法:根据O型孕妇妊娠20～24周测定的IgG抗A或(和)抗(B)效价,把新生儿分为4组(≤1∶32、1∶64、1∶128、≥1∶256),取脐血进行Coombs实验,游离抗体和抗体释放实验以证实新生儿ABO溶血病,监测新生儿血清胆红素;并采用Fanaroff教授等制定的黄疸干预方案对新生儿进行早期综合治疗。结果:当孕妇血型抗体效价为≤1∶32、1∶64、1∶128和≥1∶256时,各组新生儿ABO溶血病的发生率分别为11 1%、13 5%、22 0%和52 9%;高胆红素血症发生率为33 3%、37 8%、46 0%和76 5%;蓝光照射时间为(7 1±3 8)、(10 9±7 7)、(17 7±9 6)和(37 1±18 7)h。结论:随着孕妇IgG抗体效价增高,新生儿ABO溶血病和高胆发生率升高,需要蓝光干预的时间延长。     

罗汉果ISSR-PCR反应体系的建立

以罗汉果 ( Siraitia grosvenorii) DNA为材料 ,分析了模板 DNA,Mg2 + ,d NTPs,引物的浓度 ,Taq DNA聚合酶的用量以及循环次数对 ISSR-PCR扩增结果的影响 ,确立了稳定的、可重复的罗汉果 ISSR最佳反应体系和 PCR扩增参数 :在 2 5μL的 PCR反应体系中 ,含 2 0～ 5 0 ng模板 DNA,1 U Taq酶 ,1× PCR缓冲液 ,2 .0 mmol/L Mg Cl2 ,4种 d NTPs各 2 0 0 μmol/L,0 .5 μmol/L引物 ;PCR扩增程序为 94°C预变性 3 min,接着进行 40个循环 :94°C变性 1 min,5 2°C退火 5 0 s,72°C延伸 2 min,循环结束后 72°C延伸 7min.     

用于DNA提取的刺桫椤叶片组织保存方法研究

用一种由 1 .5 % SDS,1 0 0 m mol/ L Tris( p H8.3) ,5 0 m mol/ L EDTA( p H8.0 ) ,5 0 0 m mol/L Na Cl和 5 0 0 m mol/ Lβ-巯基乙醇组成的 SDS提取液保存野外采集的刺桫椤 ( Alsophila spinulosa)叶片组织 ,结果表明 ,保存 7d、 1 5 d、 30 d、 90 d、 1 80 d(室温下 )的刺桫椤叶片组织提得的 DNA分子量与鲜叶一致 ,均大于 4 8kb,得率为 6 0 0～ 1 2 0 0 μg/ g· FW.采自同一植株不同保存时间的叶片组织获得的 DNA用于 RAPD分析 ,结果完全一致 .用该方法保存的 8个刺桫椤天然居群的 91份样品 ,提得的 DNA已用于基于PCR技术的研究     

基因重组牛碱性成纤维细胞生长因子促进创面愈合的临床研究

目的 :观察基因重组牛碱性成纤维细胞生长因子 (rbFGF)对急性创面和慢性创面愈合的促进作用。方法 :治疗组 (12 82例 )和对照组 (439例 )被分为深Ⅱ度烧伤、浅Ⅱ度烧伤、供皮区 (包括刃厚和中厚供皮区 )和慢性 (包括残余小创面和慢性溃疡 )创面 4大类。分别观察应用rbFGF后不同时间创面愈合面积的百分比、创面完全愈合时间 ,以及用药前后的全身情况和不良反应。结果 :浅Ⅱ度烧伤 8d愈合率和创面完全愈合时间 ,对照组为 5 1 7%± 2 6 4 %和 (12 6± 2 9)d(P <0 0 0 1) ,治疗组为 70 6 %± 2 5 0 %和 (10 5± 2 4 )d(P <0 0 0 1)。深Ⅱ度烧伤 15d愈合率和创面完全愈合时间 ,对照组为 5 3 3%± 2 5 4 %和 (2 1 9± 5 5 )d(P <0 0 0 1) ,治疗组为 6 9 7%± 2 7 0 %和 (18 5± 4 4 )d(P <0 0 0 1)。供皮区创面 8d愈合率和创面完全愈合时间 ,对照组为 5 7 6 %± 2 9 4 %和(11 4± 2 7)d(P <0 0 0 1) ,治疗组为 72 1%± 2 8 0 %和 (9 5± 2 4 )d(P <0 0 0 1)。慢性创面完全愈合时间 ,对照组为 (2 8 0± 18 0 )d ,治疗组 (2 2 1± 16 )d(P =0 0 6 8)。用药前后血常规、肝、肾功能无异常变化。结论 :基因重组牛碱性成纤维细胞生长因子能够显著地促进创面     

粒细胞集落刺激因子对甘油诱导小鼠急性肾功能衰竭的保护作用

目的:探讨粒细胞集落刺激因子(G-CSF)对甘油诱导的小鼠急性肾功能衰竭的保护作用。方法:将φ=50%的甘油注射于停止饮水18h的C57BL/6j小鼠后肢肌肉内(11 6mL/kg),诱导小鼠产生急性肾功能衰竭;于注射甘油3h后,实验组小鼠每天腹腔注射G-CSF10μg/kg,连续注射5d,对照组用等量的生理盐水进行注射,正常组不作任何处理;在注射甘油72h和168h后,分别检测小鼠血清的肌酐和尿素氮含量,观察小鼠肾脏组织的病理变化。结果:在注射甘油后第72h,实验组血清的肌酐和尿素氮分别为(37 2±2 17)μmol/L和(15 6±1 60)mmol/L,显著低于对照组(P<0 01),略高于正常组(P>0 05);肾组织切片,HE染色,显微镜检查发现肾近曲小管上皮细胞变性坏死病灶呈散在分布(++),明显较对照组(++++)轻。在第168h,实验组血清的肌酐和尿素氮分别为(32 3±3 46)μmol/L和(10 90±1 24)mmol/L,接近于正常(32 2±2 09)μmol/L和(12 25±1 11)mmol/L;肾组织切片,HE染色,显微镜检查发现肾组织病理改变(+)也接近正常。结论:G-CSF对甘油诱导的小鼠急性肾功能衰竭有保护作用。     

新生儿高未结合胆红素病因及治疗探讨

新生儿黄疸是新生儿,尤其是出生后一周以内的新生儿最常见的症状。足月儿血中胆红素超过205μmol/L(12mg/dl),早产儿超过256μmol/L(15mg/dl),称为高胆红素血症。当未结合胆红素异常升高时,会发生核黄疸或脑组织损害,严重威胁新生儿的健康和生命。现将我院1994年5月至1995年5月收住的40例病例分析如下。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Jane Eyre——A Feminist In The 19th Century

In the 19th century the society was controlled by men, and women were just appendants of them, they had not any rights and freedom. But Jane was an exception, she showed some characteristics of early feminist. Jane showed her characteristics of feminism in three aspects: rebellion, equality, and independence. These characteristics were helpful to her success, and feminism is the only way out for women of that time.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-